Reductive transformation of methyl parathion by the cyanobacterium Anabaena sp. strain PCC7120

Barton, John M.; Kuritz, Tanya; O’Connor, L. E.; Y., C.; Maskarinec, M.P.; Davison, Brian H.

doi:10.1007/s00253-004-1557-y

Cited by 46 publications

(27 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reductive transformation of organophosphates that have nitro group, such as parathion (39), (41), and EPN (70), takes place in snails, crabs, Daphnia, and algae (Francis et al 1980;Megharaj et al 1994;Mostafa et al 1991;Takimoto et al 1987a). Barton et al (2004) confirmed that the nitroso derivative of (39) served as a reductive intermediate when metabolized by the blue-green alga Anabaena sp. Reductive debromination of leptophos (71) was reported by Francis et al (1980) in a model ecosystem study.…”

Section: Organophosphorus Pesticidessupporting

confidence: 75%

“…A similar approach was undertaken by using Anabaena sp. to examine the reduction mechanism of methyl parathion (39) (Barton et al 2004). A gene mutation relating to nitrate reduction did not affect the degradation of (39), and the transient formation of the nitroso derivative in the presence of light probability proceeded by the action of ferredoxin-NADP + reductase.…”

Section: Reductasesmentioning

confidence: 99%

See 1 more Smart Citation

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Katagi

2009

Reviews of Environmental Contamination and Toxicology

136

102

View full text Add to dashboard Cite

The ecotoxicological assessment of pesticide effects in the aquatic environment should normally be based on a deep knowledge of not only the concentration of pesticides and metabolites found but also on the influence of key abiotic and biotic processes that effect rates of dissipation. Although the bioconcentration and bioaccumulation potentials of pesticides in aquatic organisms are conveniently estimated from their hydrophobicity (represented by log K(ow), it is still indispensable to factor in the effects of key abiotic and biotic processes on such pesticides to gain a more precise understanding of how they may have in the natural environment. Relying only on pesticide hydrophobicity may produce an erroneous environmental impact assessment. Several factors affect rates of pesticide dissipation and accumulation in the aquatic environment. Such factors include the amount and type of sediment present in the water and type of diet available to water-dwelling organisms. The particular physiological behavior profiles of aquatic organisms in water, such as capacity for uptake, metabolism, and elimination, are also compelling factors, as is the chemistry of the water. When evaluating pesticide uptake and bioconcentration processes, it is important to know the amount and nature of bottom sediments present and the propensity that the stuffed aquatic organisms have to absorb and process xenobiotics. Extremely hydrophobic pesticides such as the organochlorines and pyrethroids are susceptible to adsorb strongly to dissolved organic matter associated with bottom sediment. Such absorption reduces the bioavailable fraction of pesticide dissolved in the water column and reduces the probable ecotoxicological impact on aquatic organisms living the water. In contrast, sediment dweller may suffer from higher levels of direct exposure to a pesticide, unless it is rapidly degraded in sediment. Metabolism is important to bioconcentration and bioaccumulation processes, as is detoxification and bioactivation. Hydrophobic pesticides that are expected to be highly stored in tissues would not be bioconcentrated if susceptible to biotic transformation by aquatic organisms to more rapidly metabolized to hydrophilic entities are generally less toxic. By analogy, pesticides that are metabolized to similar entities by aquatic species surely are les ecotoxicologically significant. One feature of fish and other aquatic species that makes them more relevant as targets of environmental studies and of regulation is that they may not only become contaminated by pesticides or other chemicals, but that they constitute and important part of the human diet. In this chapter, we provide an overview of the enzymes that are capable of metabolizing or otherwise assisting in the removal of xenobiotics from aquatic species. Many studies have been performed on the enzymes that are responsible for metabolizing xenobiotics. In addition to the use of conventional biochemical methods, such studies on enzymes are increasingly being conducted using immunochemical met...

show abstract

Section: Organophosphorus Pesticidessupporting

confidence: 75%

Section: Reductasesmentioning

confidence: 99%

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Katagi

2009

Reviews of Environmental Contamination and Toxicology

136

102

View full text Add to dashboard Cite

show abstract

“…Although in most of the studies on microbial degradation of parathion and methyl parathion, the first reaction was hydrolysis of the phosphotriester bond, there have been reports of different degradation pathways (Barton et al 2004;Munnecke and Hsieh 1976). In this work, we report that Bacillus sp.…”

Section: Introductionmentioning

confidence: 98%

Reductive transformation of parathion and methyl parathion by Bacillus sp.

et al. 2007

View full text Add to dashboard Cite

Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences and BIOLOG test, a soil bacterium was identified as Bacillus sp. DM-1. Using either growing cells or a cell-free extract, it transformed parathion and methyl parathion to amino derivatives by reducing the nitro group. Pesticide transformation by a cell-free extract was specifically inhibited by three nitroreductase inhibitors, indicating the presence of nitroreductase activity. The nitroreductase activity was NAD(P)H-dependent, O(2)-insensitive, and exhibited the substrate specificity for parathion and methyl parathion. Reductive transformation significantly decreased the toxicity of pesticides.

show abstract

“…Algae are known to be metabolically competent and active in the degradation of CPPs and other organic xenobiotics [4][5][6]. They are also ubiquitous in their distribution and are a vital part of nutrient cycles, involved in the fixation of both carbon and nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Assessing the potential for algae and macrophytes to degrade crop protection products in aquatic ecosystems

Thomas

Hand

2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Rates of pesticide degradation in aquatic ecosystems often differ between those observed within laboratory studies and field trials. Under field conditions, a number of additional processes may well have a significant role, yet are excluded from standard laboratory studies, for example, metabolism by aquatic plants, phytoplankton, and periphyton. These constituents of natural aquatic ecosystems have been shown to be capable of metabolizing a range of crop protection products. Here we report the rate of degradation of six crop protection products assessed in parallel in three systems, under reproducible, defined laboratory conditions, designed to compare aquatic sediment systems which exclude macrophytes and algae against those in which macrophytes and/or algae are included. All three systems remained as close as possible to the Organisation for Economic Co-operation and Development (OECD) 308 guidelines, assessing degradation of parent compound in the total system in mass balanced studies using ((14) C) labeled compounds. We observed, in all cases where estimated, significant increases in the rate of degradation in both the algae and macrophyte systems when compared to the standard systems. By assessing total system degradation within closed, mass balanced studies, we have shown that rates of degradation are enhanced in water/sediment systems that include macrophytes and algae. The contribution of these communities should therefore be considered if the aquatic fate of pesticides is to be fully understood.

show abstract

Reductive transformation of methyl parathion by the cyanobacterium Anabaena sp. strain PCC7120

Cited by 46 publications

References 25 publications

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Reductive transformation of parathion and methyl parathion by Bacillus sp.

Assessing the potential for algae and macrophytes to degrade crop protection products in aquatic ecosystems

Contact Info

Product

Resources

About