Reductive diazotation of carbon nanotubes: an experimental and theoretical selectivity study

Abstract

The reaction of negatively charged SWCNTs with diazonium salts was analyzed in a combined experimental and computational DFT study.

Chemically Cross-Linked Carbon Nanotube Films Engineered to Control Neuronal Signaling

“…As shown in Figure S2d, where the RBM spectra are normalized to the mode of the nanotube with the largest diameter that was not affected by the functionalization (smallest Raman shift), the RBM intensities indicate that metallic CNTs are affected by the cross-linking process to a larger extent when compared to semiconducting CNTs. These results are in good agreement with previous studies that have revealed that the covalent functionalization of SWCNTs with diazonium salts takes place with high selectivity toward metallic CNTs. , …”

“…Primary dissociated cultures were prepared from postnatal (P2–P3) rats as previously reported. ,, All procedures were approved by the local veterinary authorities and performed in accordance with the Italian law (decree 116/92) and the EU guidelines (86/609/CE, 2007/526/CE, and 2010/63/UE). Animal use was approved by the Italian Ministry of Health.…”

“…Cultures were loaded with the cell-permeable Ca 2+ dye Oregon Green 488 BAPTA-1 AM (Invitrogen). A 4 mM stock solution of the Ca 2+ dye was prepared in DMSO, and cultures were incubated with a final concentration of 4 μM for 40 min (37 °C; 5% CO 2 ). , The samples were then placed in a recording chamber mounted on an inverted microscope (Nikon Eclipse Ti-U), where they were continuously superfused at room temperature (RT) by recording a solution of the following composition (mM): 150 NaCl, 4 KCl, 1 MgCl 2 , 2 CaCl 2 , 10 HEPES, 10 glucose (pH adjusted to 7.4 with NaOH), to continuously supply nutrients to the tissue. Cultures were observed with a 20× objective (0.45 NA), and recordings were performed from visual fields (680 × 680 μm 2 , binning 4).…”

“…Primary dissociated cultures were prepared from postnatal (P2-P3) rats as previously reported. 17,30,42 36,63 and at the end sterilized with an UV lamp.…”

“…4 mM stock solution of the Ca 2+ dye was prepared in DMSO and cultures were incubated with a final concentration of 4 µM for 40 min (37 °C; 5% CO2). 30,42 The samples were then placed in a recording chamber mounted on an inverted microscope (Nikon Eclipse Ca 2+ transients were expressed as fractional amplitude increase (ΔF/F0, where F0 is the baseline fluorescence level and ΔF is the rise over baseline); we determine the onset time of neuronal activation by detecting those events in the fluorescence signal that exceed at least five times the standard deviation of the noise.…”