Reductive Dehalogenation of Chlorobenzene Congeners in Cell Extracts of
            <i>Dehalococcoides</i>
            sp. Strain CBDB1

Hölscher, Tina; Görisch, Helmut; Adrian, Lorenz

doi:10.1128/aem.69.5.2999-3001.2003

Cited by 75 publications

(76 citation statements)

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“…Reductive dechlorination of chlorinated benzenes and PCDDs which had previously been shown for strain CBDB1 (2,9,18) was also recently demonstrated for strain 195 (11). However, different patterns of chlorobenzene dechlorination (3) and differences in the utilization of chlorobenzene and PCDD congeners have been observed for strain CBDB1 (2,9,17,18) and strain 195 (11). For example, hexachlorobenzene (HCB) dechlorination by strain 195 mainly follows the pattern HCB3pentachlorobenzene (PeCB)31,2,3,5-tetrachlorobenzene (TeCB)31,3,5-trichlorobenzene (TCB) (11) that was also described for the Dehalococcoides-like bacterium DF-1 identified in a mixed culture (50).…”

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confidence: 83%

“…For example, hexachlorobenzene (HCB) dechlorination by strain 195 mainly follows the pattern HCB3pentachlorobenzene (PeCB)31,2,3,5-tetrachlorobenzene (TeCB)31,3,5-trichlorobenzene (TCB) (11) that was also described for the Dehalococcoides-like bacterium DF-1 identified in a mixed culture (50). In contrast, HCB dechlorination in strain CBDB1 proceeds via the two isomers 1,2,4,5-TeCB and 1,2,3,5-TeCB and leads to the formation of dichlorobenzenes and 1,3,5-TCB (17,18). In contrast to strain 195 and bacterium DF-1, strain CBDB1 is able to grow with 1,2,3-TCB and 1,2,4-TCB as the sole electron acceptors (2).…”

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confidence: 99%

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Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides

Hölscher

Krajmalnik‐Brown²,

Ritalahti³

et al. 2004

Appl Environ Microbiol

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128

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Degenerate primers were used to amplify large fragments of reductive-dehalogenase-homologous (RDH) genes from genomic DNA of two Dehalococcoides populations, the chlorobenzene-and dioxin-dechlorinating strain CBDB1 and the trichloroethene-dechlorinating strain FL2. The amplicons (1,350 to 1,495 bp) corresponded to nearly complete open reading frames of known reductive dehalogenase genes and short fragments (approximately 90 bp) of genes encoding putative membrane-anchoring proteins. Cloning and restriction analysis revealed the presence of at least 14 different RDH genes in each strain. All amplified RDH genes showed sequence similarity with known reductive dehalogenase genes over the whole length of the sequence and shared all characteristics described for reductive dehalogenases. Deduced amino acid sequences of seven RDH genes from strain CBDB1 were 98.5 to 100% identical to seven different RDH genes from strain FL2, suggesting that both strains have an overlapping substrate range. All RDH genes identified in strains CBDB1 and FL2 were related to the RDH genes present in the genomes of Dehalococcoides ethenogenes strain 195 and Dehalococcoides sp. strain BAV1; however, sequence identity did not exceed 94.4 and 93.1%, respectively. The presence of RDH genes in strains CBDB1, FL2, and BAV1 that have no orthologs in strain 195 suggests that these strains possess dechlorination activities not present in strain 195. Comparative sequence analysis identified consensus sequences for cobalamin binding in deduced amino acid sequences of seven RDH genes. In conclusion, this study demonstrates that the presence of multiple nonidentical RDH genes is characteristic of Dehalococcoides strains.

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confidence: 83%

mentioning

confidence: 99%

Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides

Hölscher

Krajmalnik‐Brown²,

Ritalahti³

et al. 2004

Appl Environ Microbiol

Self Cite

128

122

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“…Most RDases contain a corrinoid cofactor and 2 ironsulfur clusters. In fact, the RDase activity of most dehalorespiring species is light-reversibly inhibited by alkyl iodides, indicating the involvement of corrinoids as a cofactor 69,86) . The rdh genes are mostly adjacent to the genes for transcriptional regulators such as two-component regulatory systems or transcriptional regulators of the MarR-type, indicating that the expression of the rdhAB genes is tightly regulated.…”

Section: Genomic Diversity Of Dehalorespirationmentioning

confidence: 99%

“…The N termini of RDases contain a twin-arginine signal sequence with the consensus motif RRXFXX, which is involved in transporting cofactor-containing proteins across the cytoplasmic membrane. Actually, TCE-RDase of "Dehalococcoides ethenogenes" 85) , the chlorobenzeneRDase of "Dehalococcoides ethenogenes" strain CBDB1 6,69) , and PceA-RDase of Desulfitobacterium hafniense strain Y51 121) are located on the exterior of the cytoplasmic membrane. Most RDases contain a corrinoid cofactor and 2 ironsulfur clusters.…”

Section: Genomic Diversity Of Dehalorespirationmentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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“…obtained in pure culture, strain CBDB1, (98.3% identity over the 16S rRNA gene sequence of strain 195 and CBDB1), was the first reported isolate able to obtain energy from chlorobenzene reductive dechlorination (Adrian et al, 2000;Hölscher et al, 2003;Jayachandran et al, 2003). Strain CBDB1 can also reductively dechlorinate selected chlorinated dibenzo-p-dioxins (Bunge et al, 2003) and chlorophenol congeners .…”

Section: Introductionmentioning

confidence: 99%

Methods and Applications

Arveson

2002

Graduate Texts in Mathematics

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Dehalococcoides species are responsible for the reductive dehalogenation of an impressive range of common, persistent environmental contaminants. These microorganisms are difficult to both obtain and grow in pure culture, and so are often studied while they exist in consortia using molecular techniques. In particular, a significant number of quantitative real-time PCR (qPCR) assays targeting Dehalococcoides spp. have been reported. Initial qPCR methods targeted the 16S rRNA gene, however, because strains with the same 16S rRNA gene sequence can have different dehalogenating abilities, reductive dehalogenase genes are now emerging as the most appropriate qPCR target. Quantitative PCR has been critical to our current understanding of Dehalococcoides populations; it has provided information on their growth characteristics, dehalogenating abilities and effective use in bioremediation efforts. Future qPCR research directions will likely involve method standardization, as well as continued research on the functional genes associated with Dehalococcoides populations.

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Reductive Dehalogenation of Chlorobenzene Congeners in Cell Extracts of Dehalococcoides sp. Strain CBDB1

Cited by 75 publications

References 25 publications

Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides

Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Methods and Applications

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