Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides

Jugder, Bat-Erdene; Ertan, Haluk; Lee, Matthew; Manefield, Michael; Marquis, Christopher P.

doi:10.1016/j.tibtech.2015.07.004

Cited by 95 publications

(86 citation statements)

References 104 publications

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“…This configuration is also found in PceA from PER-K23 and Desulfitobacterium hafniense strain TCE1 (Maillard et al, 2005; Duret et al, 2012). In other cases, the rdhT , e.g., in strain UNSWDHB, it is outside the rdhA gene cluster (Jugder et al, 2015, 2016a; Wong et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

“…Rdh genes are normally clustered in an operon encoding different parts of the RDases system ( rhdABCT ); rdhA encodes for the catalytic subunit of the enzyme, rdhB is the membrane-anchoring protein for RdhA. rdhC is believed to be a transcriptional regulator for RdhA and rhdT encodes for a chaperone protein for the proper folding of RdhA and its exportation to the periplasm through the TAT secretion pathway (Jugder et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

“…It has been recently demonstrated explicitly that the cobalt atom in the cobalamin catalyzes the reduction of the halogen in the substrate (Payne et al, 2015). Two Fe-S clusters are present along with a twin-arginine (TAT) motif (RRXFXK) at the N-terminus that is recognized by RdhT for folding and exportation (Rupakula et al, 2013; Jugder et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase

et al. 2017

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Dehalobacter sp. strain TeCB1 was isolated from groundwater near Sydney, Australia, that is polluted with a range of organochlorines. The isolated strain is able to grow by reductive dechlorination of 1,2,4,5-tetrachlorobenzene to 1,3- and 1,4-dichlorobenzene with 1,2,4-trichlorobenzene being the intermediate daughter product. Transient production of 1,2-dichlorobenzene was detected with subsequent conversion to monochlorobenzene. The dehalogenation capability of strain TeCB1 to respire 23 alternative organochlorines was examined and shown to be limited to the use of 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene. Growth on 1,2,4-trichlorobenzene resulted in the production of predominantly 1,3- and 1,4-dichlorobenzene. The inability of strain TeCB1 to grow on 1,2-dichlorobenzene indicated that the production of monochlorobenzene during growth on 1,2,4,5-tetarchlorobezene was cometabolic. The annotated genome of strain TeCB1 contained only one detectable 16S rRNA gene copy and genes for 23 full-length and one truncated Reductive Dehalogenase (RDase) homologs, five unique to strain TeCB1. Identification and functional characterization of the 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene RDase (TcbA) was achieved using native-PAGE coupled with liquid chromatography tandem mass spectrometry. Interestingly, TcbA showed higher amino acid identity with tetrachloroethene reductases PceA (95% identity) from Dehalobacter restrictus PER-K23 and Desulfitobacterium hafniense Y51 than with the only other chlorinated benzene reductase [i.e., CbrA (30% identity)] functionally characterized to date.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase

et al. 2017

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“…Members of these orders have already been retrieved in PCB polluted soils, involved in biphenyl degradation and for some genera also in PCB degradation (Leigh et al, 2007; Hu et al, 2015; Jayanna and Gayathri, 2015; Leewis et al, 2016b). The phylum Chloroflexi , comprising a well known group of anaerobic PCB degraders (Jugder et al, 2015), was retrieved at relative abundance higher than 1% exclusively in the S fractions, being particularly enriched in MS1. It is possible that an anoxic microniche occurred in this sample, allowing the selection of Chloroflexi members, in agreement with previous finding of Chloroflexi enrichment in the rhizosphere of M. sativa during a PCB polluted soil phytoremediation trial (Tu et al, 2011) and of Sparganium sp.…”

Section: Discussionmentioning

confidence: 99%

Bacteria Associated to Plants Naturally Selected in a Historical PCB Polluted Soil Show Potential to Sustain Natural Attenuation

et al. 2017

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The exploitation of the association between plants and microorganisms is a promising approach able to boost natural attenuation processes for soil clean-up in vast polluted areas characterized by mixed chemical contamination. We aimed to explore the selection of root-associated bacterial communities driven by different plant species spontaneously established in abandoned agricultural soils within a historical polluted site in north Italy. The site is highly contaminated by chlorinated persistent organic pollutants, mainly constituted by polychlorobiphenyls (PCBs), together with heavy metals and metalloids, in variable concentrations and uneven distribution. The overall structure of the non-vegetated and root-associated soil fractions bacterial communities was described by high-throughput sequencing of the 16S rRNA gene, and a collection of 165 rhizobacterial isolates able to use biphenyl as unique carbon source was assayed for plant growth promotion (PGP) traits and bioremediation potential. The results showed that the recruitment of specific bacterial communities in the root-associated soil fractions was driven by both soil fractions and plant species, explaining 21 and 18% of the total bacterial microbiome variation, respectively. PCR-based detection in the soil metagenome of bacterial bphA gene, encoding for the biphenyl dioxygenase α subunit, indicated that the soil in the site possesses metabolic traits linked to PCB degradation. Biphenyl-utilizing bacteria isolated from the rhizosphere of the three different plant species showed low phylogenetic diversity and well represented functional traits, in terms of PGP and bioremediation potential. On average, 72% of the strains harbored the bphA gene and/or displayed catechol 2,3-dioxygenase activity, involved in aromatic ring cleavage. PGP traits, including 1-aminocyclopropane-1-carboxylic acid deaminase activity potentially associated to plant stress tolerance induction, were widely distributed among the isolates according to in vitro assays. PGP tested in vivo on tomato plants using eleven selected bacterial isolates, confirmed the promotion and protection potential of the rhizosphere bacteria. Different spontaneous plant species naturally selected in a historical chronically polluted site showed to determine the enrichment of peculiar bacterial communities in the soil fractions associated to the roots. All the rhizosphere communities, nevertheless, hosted bacteria with degradation/detoxification and PGP potential, putatively sustaining the natural attenuation process.

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“…(13) Lastly, formation of cob(II)alamin halide adduct was recently suggested from structure–function studies of two bacterial reductive dehalogenases. (14)…”

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confidence: 99%

Enzymatic Reductive Dehalogenation Controls the Biosynthesis of Marine Bacterial Pyrroles

et al. 2016

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Enzymes capable of performing dehalogenating reactions have attracted tremendous contemporary attention due to their potential application in the bioremediation of anthropogenic polyhalogenated persistent organic pollutants. Nature, in particular the marine environment, is also a prolific source of polyhalogenated organic natural products. The study of the biosynthesis of these natural products has furnished a diverse array of halogenation biocatalysts, but thus far no examples of dehalogenating enzymes have been reported from a secondary metabolic pathway. Here we show that the penultimate step in the biosynthesis of the highly brominated marine bacterial product pentabromopseudilin is catalyzed by an unusual debrominase Bmp8 that utilizes a redox thiol mechanism to remove the C-2 bromine atom of 2,3,4,5-tetrabromopyrrole to facilitate oxidative coupling to 2,4-dibromophenol. To the best of our knowledge, Bmp8 is first example of a dehalogenating enzyme from the established genetic and biochemical context of a natural product biosynthetic pathway.

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Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides

Cited by 95 publications

References 104 publications

Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase

Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase

Bacteria Associated to Plants Naturally Selected in a Historical PCB Polluted Soil Show Potential to Sustain Natural Attenuation

Enzymatic Reductive Dehalogenation Controls the Biosynthesis of Marine Bacterial Pyrroles

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