Reductive dechlorination of a polychlorinated biphenyl congener and hexachlorobenzene by vitamin B12

Assaf-Anid, Nada; Nies, Loring; Vogel, Timothy M.

doi:10.1128/aem.58.3.1057-1060.1992

Cited by 72 publications

(36 citation statements)

References 19 publications

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“…Since the addition of amorphous FeS to autoclaved sterile controls resulted in the observance of inconsequential abiotic effects, no further discussion of abiotic effects of metal sulfides shall be considered in the current context. However, iron itself has been demonstrated to play a role in the reductive dehalogenation of alkyl and aryl chlorinated compounds in biochemical cell-free systems such as iron porphyrins, hematin, and vitamin B 12 [30][31][32]. While association between FeS and such iron-containing systems is hypothetical and unclear at best, the potential warrants further investigation.…”

Section: Discussionmentioning

confidence: 99%

Effects of selected reducing agents on microbiologically mediated reductive dechlorination of aroclor® 1242

McCue¹,

Gauger²,

Holsen³

et al. 1996

Enviro Toxic and Chemistry

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The effect of various chemical reducing agents on the anaerobic microbial reductive biotransformation of polychlorinated biphenyls (PCBs) was investigated using Aroclor® 1242 at a concentration of 600 μg/g soil and microorganisms eluted from Hudson River sediments. The investigation sought to determine how various reducing agents influenced PCB dechlorination rates, patterns, and acclimation periods. Three reducing agents were incorporated into culture media: amorphous ferrous sulfide, sodium sulfide with sodium thioglycollate, and sodium sulfide with amorphous ferrous sulfide. All cultures were incubated statically under methanogenic conditions and ambient incubation temperatures of about 20°C. Cultures utilizing both sodium sulfide and ferrous sulfide together demonstrated the most extensive removal of meta‐ and para‐chlorines, the highest average dechlorination rate, the greatest dechlorination of tri‐ and tetrachlorinated biphenyls, and the greatest accumulation of dichlorobiphenyl homologues. Overall, treatments incorporating ferrous sulfide, alone or in combination with sodium sulfide, demonstrated the ability to more effectively dechlorinate tri‐ortho‐substituted tetrachlorobiphenyls and pentachlorobiphenyls which had commonly positioned chlorines. Under these same conditions, dichlorobiphenyls, which are more amenable to aerobic dechlorination conditions, increased from 25% (molar basis) initially to levels greater than 60%.

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Section: Discussionmentioning

confidence: 99%

Effects of selected reducing agents on microbiologically mediated reductive dechlorination of aroclor® 1242

McCue¹,

Gauger²,

Holsen³

et al. 1996

Enviro Toxic and Chemistry

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show abstract

“…By using the purified enzyme, liberation of HCl was observed from tetrachloromethane and trichloromethane (556). Transition metal coenzymes dechlorinate not only aliphatic compounds such as polychlorinated methanes, ethanes, and ethylenes but also halobenzenes (19,147,211) or, for instance, 2,3,4,5,6-pentachlorobiphenyl (19). These data suggest that nonspecific reactions with transition metal cofactors may also be important in the degradation of xenobiotic compounds in anaerobic environments.…”

Section: Catalysis By Transition Metal Cofactors (Porphyrins and Corrmentioning

confidence: 93%

Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

Fetzner

Lingens

1994

Microbiological Reviews

260

134

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hydraulic and heat transfer fluids, plasticizers, and intermediates for chemical syntheses. Because of their toxicity, bioconcentration, and persistence, the ubiquitous distribution of halogenated compounds in the biosphere has caused public concern over the possible effects on the quality of life. Concerning toxicity, an important problem is the diversity of toxic compounds and by-products in many formulations of industrial products. This problem applies to mixtures of polychlorinated biphenyls (PCBs) or chlorinated terpenes, poly-641

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“…1990;Nies and Vogel 1991 ; Assaf-Anid el al. 1992;Flanagan and May 1993;Fish and Principe 1994;Amaro er al. 1996).…”

Section: Introductionmentioning

confidence: 99%

Rapid Microbial Growth on Polychlorinated Biphenyl and Its Fate in Cells

Hamdy

Lin

Toledo

1999

Rapid Methods Automation Mic

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Nine cultures were examined for growth at 37C in media with different levels of PCB and selected one culture to treat an industrial liquid waste containing high levels of PCB 1248. All the nine cultures grew well in the presence of 100 to 500 p g PCB 1254 mL" glucose basal salt broth (GBSB) as well as in tryptic soy broth (TSP) with no significant changes in generation time. The cultures grew well in basal salt agar containing PCB as sole source of carbon. Pseudomonas aeruginosa and Serratia liquefaciens strains survived for 75 to 120 min in the presence of 300 to 2000 p g PCB I254 mL" sodium-phosphate buffer (SPB) pH 7.0. The P. aeruginosa cells removed both PCB 1254 and 14C-PCB from SPB and GBSB within 48 h and the percent uptakes were 26 and 39.7%, respectively. The results of the fate and distribution of PCB 1254, including "C-PCB, in cells of P. aeruginosa, S. liquefaciens. and two Bacillus strains grown in PCB-media showed that more than 50% of the "C-PCB were in the alcohol and in alcohol-ether soluble fractions (mostly lipids). This was also confirmed afer rupturing the P. aeruginosa cells using French pressure and/or lysozyme and fractionation. Both the intrinsic jlora and P. aeruginosa strain were used in a bioreactor system for the treatments of 27 and 33 gallons industrial liquid waste containing 150 and/or 800 mg PCB 1248 I-', respectively, and complete oxidation was achieved, within approximately 150 days and the biological half-life of the PCB was 30 and 15 days, respectively.

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Reductive dechlorination of a polychlorinated biphenyl congener and hexachlorobenzene by vitamin B12

Cited by 72 publications

References 19 publications

Effects of selected reducing agents on microbiologically mediated reductive dechlorination of aroclor® 1242

Effects of selected reducing agents on microbiologically mediated reductive dechlorination of aroclor® 1242

Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

Rapid Microbial Growth on Polychlorinated Biphenyl and Its Fate in Cells

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