Reductive activation of mitomycin C and mitomycin C metabolites catalyzed by NADPH-cytochrome P-450 reductase and xanthine oxidase.

Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro

“…In addition, we found that PE could prevent the generation of MMC-and cDDP-induced BNCs. The underlying molecular mechanisms may be: (1) the reduction of MN and NPBs, thereby reducing the frequency of spontaneous furrow regression caused by chromatin localized to the cleavage plane (Pan et al, 1984);…”

Mode of action for mitomycin C inactivating factors of Pseudomonas convexa 4-87.

“…Tomasz and Lipman obtained these derivatives in a reaction of MMC with a rat liver microsomal fraction under anaerobic conditions.7* Pan et al 13) also found that NADPHcytochrome P-450 reductase and xanthine oxidase catalyzed MMC reduction under anaerobic conditions and with proper electron donors to produce these three metabolites. The The carbonium ion formed thus reacts in vitro with the nucleophilic center in the reaction mixture.…”

Isolation and Identification of Metabolites of Porfiromycin Formed in the Presence of a Rat Liver Preparation

“…The bioreductive activation and in vitro metabolism of MC under hypoxic conditions have been extensively investigated. [16][17][18][19][20] However, limited information on the metabolism and disposition of PM is available in the literature. 21,22 Enzymatic activity that catalyzes the reductive activation of MC/PM has been demonstrated in the microsomes, cytosol, and nuclei of rat liver cells, [16][17][18][19]21 as well as in other mammalian cells.…”

“…[16][17][18][19][20] However, limited information on the metabolism and disposition of PM is available in the literature. 21,22 Enzymatic activity that catalyzes the reductive activation of MC/PM has been demonstrated in the microsomes, cytosol, and nuclei of rat liver cells, [16][17][18][19]21 as well as in other mammalian cells. 20,[22][23][24][25][26][27] Two major enzymes responsible for the reductive activation in the microsomal and cytosolic fractions are NADPH-cytochrome c (P-450) reductase [19][20][21] and NAD(P)H:quinone oxidoreductase (DT-diaphorase) 20,23 , respectively.…”

“…21,22 Enzymatic activity that catalyzes the reductive activation of MC/PM has been demonstrated in the microsomes, cytosol, and nuclei of rat liver cells, [16][17][18][19]21 as well as in other mammalian cells. 20,[22][23][24][25][26][27] Two major enzymes responsible for the reductive activation in the microsomal and cytosolic fractions are NADPH-cytochrome c (P-450) reductase [19][20][21] and NAD(P)H:quinone oxidoreductase (DT-diaphorase) 20,23 , respectively. The former exerts its activity only in hypoxia, whereas the latter shows similar activity under both aerobic and anaerobic conditions.…”