Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels

Bustos, Dolores; Lascano, Ramiro; Villasuso, Ana Laura; Machado, Estela E.; Senn, María Eugenia; Córdoba, Alicia; Taleisnik, Edith

doi:10.1093/aob/mcn141

Cited by 29 publications

(23 citation statements)

References 59 publications

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“…Root tips of P. coloratum plants grown under alkaline conditions showed increased superoxide production (Figure a). The plasma membrane‐bound superoxide‐generating NADPH oxidase complex is mainly responsible for apoplastic superoxide production in roots (Bustos et al ., ). This complex is a key player in the ROS signalling network that participates in growth and development regulation as well as in the responses to multiple stress conditions (Suzuki et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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Nutrient deficiency and hypoxia as constraints to Panicum coloratum growth in alkaline soils

Luna

Aguirre

Pittaro

et al. 2016

Grass and Forage Science

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Alkaline and saline–alkaline soils impose severe restrictions on plant growth. Panicum coloratum var. coloratum is a perennial C4 forage grass widely used in tropical and subtropical environments. Published information on its responses to alkaline soil conditions is scarce. The objectives of this study were (i) to characterize the effects of alkaline substrates on germination and initial growth in this species, (ii) to assess the influence of high pH in combination with reduced availability of either nutrients or oxygen and salinity, on plant growth and (iii) to evaluate some physiological traits potentially responsible for growth restrictions under alkaline soil conditions. Trials were conducted in a greenhouse. Germination and early plant survival were not affected by alkalinity. To isolate the effects of high pH, reduced nutrient and oxygen availability on growth, plants were grown either in neutral or alkaline soil, in hydroponics, in neutralized alkaline soil (with or without supplementary fertilization), or were flooded to induce hypoxia. Alkalinity effects on growth in hydroponics were milder than in soil. Growth in alkaline soil with nutrient supplement was still significantly lower (by 40%) than in neutral soil. Both alkalinity and hypoxia reduced growth non‐synergistically. These results show that studies of plant response to alkaline substrates carried out in aerated nutrient solutions can only partially address the complexity of this stress. Photosynthesis and PSII activity were among the physiological mechanisms negatively affected by alkalinity and may be partially responsible for the growth limitations observed in P. coloratum under alkaline conditions.

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Section: Discussionmentioning

confidence: 97%

“…Superoxide presence in root tips was determined with nitro blue tetrazolium (NBT), following Bustos et al . (). Oxidative damage in leaves was evaluated by malondialdehyde (MDA) concentration, measured in alcohol extracts according to Heath and Parker ().…”

Section: Methodsmentioning

confidence: 97%

Nutrient deficiency and hypoxia as constraints to Panicum coloratum growth in alkaline soils

Luna

Aguirre

Pittaro

et al. 2016

Grass and Forage Science

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“…For example, growth rates may be generally decreased, maximum growth rates may be reduced or shifted proximally, and the elongation zone may be shortened (Fricke, 2002;Rodríguez et al, 2004). Reductions in growth rates are normally observed in the decelerating zone of growth rates, but rates in the growth accelerating zone, closest to the meristem, may remain unaltered (Ortega et al, 2006), as observed in roots under water stress (Sharp et al, 1988;Fan and Neumann, 2004) or salinity (Bustos et al, 2008).…”

Section: Elongation Growth In the Ezmentioning

confidence: 99%

“…Treatments with inhibitors of polyamine oxidase, a salt-tolerant enzyme, suggest that this enzyme may also play a role in maintaining leaf growth under salinity (Maiale et al, 2008). Nevertheless, in maize root tips, detailed studies have indicated that the distribution of accelerating growth and highest d O 2 À levels coincide along the root tip in non-stressed plants, while growth changes due to stress and the distribution of apoplastic d O 2 À are not associated (Bustos et al, 2008).…”

Section: Ros and The Regulation Of Leaf Growth Under Stressmentioning

confidence: 99%

“…The differential effect of salt and osmotic stress on ROS production in leaves (Rodríguez et al, 2004) and roots (Bustos et al, 2008) is part of this set of specific stress responses. In maize roots, differences in apoplastic .O 2 À production may participate in signaling processes that convey information on the nature of the substrate the growing root is exploring (Bustos et al, 2008).…”

Section: Ros and The Regulation Of Leaf Growth Under Stressmentioning

confidence: 99%

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Leaf expansion in grasses under salt stress

Taleisnik

Rodríguez

Bustos

et al. 2009

Journal of Plant Physiology

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Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues

Rodríguez

Taleisnik

2012

Methods in Molecular Biology

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Reactive oxygen species (ROS) participate in signaling events that regulate ion channel activity and gene expression. However, excess ROS exert adverse effects that stem from their interaction with macromolecules. Thus, the assessment of the effects of salinity on ROS changes are central to understanding how plants respond and cope with this stress. ROS determination in salt-stressed plants poses specific challenges. On the one hand, salinity comprises osmotic and ion-specific effects which may, in turn, have different effects on ROS production. On the other hand, changes in ROS production may happen when tissues from salinized plants are subject to water potential (Ψ) changes when incubated in non-isosmotic solutions. This chapter provides detailed accounts of methods for ROS detection in tissues from salt-stressed plants and includes suggestions for avoiding artifacts when dealing with such tissues.

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Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels

Cited by 29 publications

References 59 publications

Nutrient deficiency and hypoxia as constraints to Panicum coloratum growth in alkaline soils

Nutrient deficiency and hypoxia as constraints to Panicum coloratum growth in alkaline soils

Leaf expansion in grasses under salt stress

Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues

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