Reductions in hepatic vitellogenin and estrogen receptor alpha expression by sediments from an agriculturally impacted waterway

Sellin, Marlo K.; Snow, Daniel D.; Kolok, Alan S.

doi:10.1016/j.aquatox.2009.10.004

Cited by 34 publications

(46 citation statements)

References 31 publications

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“…Sediment samples were extracted using microwave assisted solvent extraction as described in Sellin et al [25]. Extracts were analyzed for steroid hormones using LC/MS/MS with atmospheric pressure photoionization.…”

Section: Steroid Hormone and Pesticide Analysismentioning

confidence: 99%

Occurrence and endocrine effects of agrichemicals in a small Nebraska, USA, watershed

Jeffries

Abbott

Cowman

et al. 2011

Enviro Toxic and Chemistry

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The Bow Creek watershed (Nebraska, USA) is dominated by the production of beef cattle and row crops; therefore, surface waters are likely to receive runoff containing steroid hormones and pesticides. The goal of the present study was to determine the occurrence and endocrine effects of agrichemicals in this watershed. To accomplish this, four sites within the watershed-Pearl, Bow, and East Bow Creeks and a site at the confluence with the Missouri River-were selected. In June of 2008, polar organic chemical integrative samplers (POCIS) were deployed at each site, whereas in June of 2009, water and sediment samples were collected. Caged fathead minnows (Pimephales promelas) were deployed at all of the selected sites in both years. Analysis of these samples revealed that steroid hormones were not present; however, pesticides were present in POCIS extracts and water samples. In general, the amount of pesticides was higher in POCIS retrieved from Pearl and Bow Creeks than in POCIS from East Bow Creek and the confluence. This variation between sites appeared to be related to row crop density, as row crop land cover surrounding the Pearl and Bow Creek sites was higher than that surrounding the East Bow and confluence sites. To determine the endocrine effects of agrichemicals within this watershed, the hepatic mRNA expression of vitellogenin and estrogen receptor α (ERα), as well as the gonadal expression of P450 aromatase A, was determined for the caged minnows. Females deployed at East Bow Creek and the confluence experienced decreases in the expression of ERα, suggesting that these females had been defeminized; however, this defeminization could not be attributed to any of the pesticides detected at these sites.

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Section: Steroid Hormone and Pesticide Analysismentioning

confidence: 99%

Occurrence and endocrine effects of agrichemicals in a small Nebraska, USA, watershed

Jeffries

Abbott

Cowman

et al. 2011

Enviro Toxic and Chemistry

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“…Hormone transport from agricultural fields and feedlot surfaces has been documented during irrigation and precipitation events [5][6][7]. The presence of steroidogenic compounds in surface water and the corresponding endocrine-disrupting effects on aquatic species has been documented adjacent to animal production facilities [8][9][10]. Due to their hydrophobicity, hormones will predominantly sorb to sediment which act as potential sinks and sources of hormones and exposure to hormones bound to sediment can elicit biologic effects in aquatic organisms, either by acting as a source of free hormone through desorption from the sediments and/or by direct contact between fish and sediment-associated hormone [11].…”

Section: Introductionmentioning

confidence: 99%

The effect of particle size on sorption of estrogens, androgens and progestagens in aquatic sediment

Sangster

Oke

Zhang

et al. 2015

Journal of Hazardous Materials

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“…In our previous studies, we also found that the levels of ERα and Vtg-I mRNAs in the liver of medaka (O. latipes) exposed to EV and estrone (E1) can be altered (Lei et al, 2013a;. Therefore, Vtg and ER genes have been widely used as marker genes to evaluate the effects of EDCs on fish (Zhang et al, 2008a;Chen et al, 2010;Sellin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000 ng/L E3 for 15 days, and the hatched fry were exposed continuously to the same concentrations for an additional 15 days. Exposure to 500 and 5000 ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000 ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000 ng/L E3 for further 60 days, the male hepatosomatic index (HSI) was increased at 5000 ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000 ng/L E3, while the male GSI at 5000 ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000 ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5 ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

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Reductions in hepatic vitellogenin and estrogen receptor alpha expression by sediments from an agriculturally impacted waterway

Cited by 34 publications

References 31 publications

Occurrence and endocrine effects of agrichemicals in a small Nebraska, USA, watershed

Occurrence and endocrine effects of agrichemicals in a small Nebraska, USA, watershed

The effect of particle size on sorption of estrogens, androgens and progestagens in aquatic sediment

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

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