Reduction-sensitive core-cross-linked mPEG–poly(ester-carbonate) micelles for glutathione-triggered intracellular drug release

Yan, Lesan; Wu, Wenbin; Zhao, Wei; Qi, Ruogu; Cui, Dongmei; Xie, Zhigang; Huang, Yubin; Tong, Ti; Jing, Xiabin

doi:10.1039/c2py20240a

Cited by 74 publications

(57 citation statements)

References 45 publications

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“…The in vitro cytotoxicity of all drugs was assessed by using the MTT assay as described previously [34]. Briefly, cells harvested in a logarithmic growth phase were seeded in 96-well plates at a density of 10 4 PBS at a concentration of 5 mg ml −1 was added and the plates were incubated for another 4 h at 37 • C, followed by removal of the culture medium containing MTT.…”

Section: Mtt (3-(45-dimethylthiazol-2-yl)-25-diphenyltetrazolium Brmentioning

confidence: 99%

Sensitizing nanoparticle based platinum(IV) drugs by curcumin for better chemotherapy

Kang

Zhao

Yan

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Section: Mtt (3-(45-dimethylthiazol-2-yl)-25-diphenyltetrazolium Brmentioning

confidence: 99%

Sensitizing nanoparticle based platinum(IV) drugs by curcumin for better chemotherapy

Kang

Zhao

Yan

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…CMC was detected by using pyrene as a probe with fluorescence spectroscopy [26]. Three kinds of blank conjugates were dissolved by deionized water.…”

Section: Critical Micelle Concentration (Cmc)mentioning

confidence: 99%

Preparation and Evaluation of Doxorubicin-Loaded Micelles Based on Glycyrrhetinic Acid Modified Gelatin Conjugates for Targeting Hepatocellular Carcinoma

Fan

Yan

et al. 2018

Journal of Nanomaterials

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Hepatocellular carcinoma (HCC) is one of the most prevalent fatal diseases and the incidence of HCC is increasing worldwide. Polymeric micelles with targeting groups have drawn great attention as carriers for drug delivery in HCC therapy. Herein, novel glycyrrhetinic acid modified gelatin (GA-GEL) conjugates with three substitution degrees were synthesized and characterized. Doxorubicin (DOX) was applied as a model drug. DOX-loaded GA-GEL (DOX/GA-GEL) micelles were prepared by an emulsionsolvent evaporation method. The mean diameters of DOX/GA-GEL micelles were in the range of 195-235 nm. The encapsulation efficiency of DOX/GA-GEL micelles was 63.6%-96.2%, and the loading content was 8.3%-12.5%. Drug release from DOX-loaded micelles exhibited a biphasic manner in phosphate buffer solution (PBS) at pH 7.4. DOX/GA-GEL could be efficiently accumulated into human liver cancer HepG2 cells. The IC 50 values of DOX/GA-GEL-2 and DOX⋅HCl in HepG2 cells were 0.33 and 0.66 g/mL, respectively. In vivo imaging analysis demonstrated that the fluorescence signals of DiR-labeled GA-GEL-2 micelles were mainly distributed in liver and H22 orthotopic tumor, indicating that GA-GEL had the liver-targeting activity. Compared to DOX⋅HCl, DOX/GA-GEL-2 exhibited better antitumor activity in H22 orthotopic mice. Therefore, these results indicated that GA-GEL could be used as carrier of hydrophobic drug for targeting HCC.

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“…[8][9][10] Environmentally, responsive technologies (eg, those based on pH sensitivity, thermal sensitivity, and redox sensitivity), combined with covalent cross-linking, have exhibited outstanding performance in the controlled release of encapsulated drugs. [11][12][13][14][15] In addition, ionic cross-linking, namely the electrostatic interaction between the positive and negative charges of the hydrophobic polymer blocks, is also a good choice in the micellar core. 16 On the other hand, weakly acidic interstitial fluid (pH 6.75-7.23) in solid tumors and acidic endosomes/ lysosomes (pH 4.0-5.5) in cells possess the characteristic acidic environment.…”

Section: Introductionmentioning

confidence: 99%

Acid-triggered core cross-linked nanomicelles for targeted drug delivery and magnetic resonance imaging in liver cancer cells

Li¹,

Li²,

Yi³

et al. 2013

IJN

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Purpose:To research the acid-triggered core cross-linked folate-poly(ethylene glycol)-b-poly [N-(N′,N′-diisopropylaminoethyl) glutamine] (folated-PEG-P[GA-DIP]) amphiphilic block copolymer for targeted drug delivery and magnetic resonance imaging (MRI) in liver cancer cells. Methods:As an appropriate receptor of protons, the N,N-diisopropyl tertiary amine group (DIP) was chosen to conjugate with the side carboxyl groups of poly(ethylene glycol)-b-poly (L-glutamic acid) to obtain PEG-P(GA-DIP) amphiphilic block copolymers. By ultrasonic emulsification, PEG-P(GA-DIP) could be self-assembled to form nanosized micelles loading doxorubicin (DOX) and superparamagnetic iron oxide nanoparticles (SPIONs) in aqueous solution. When PEG-P(GA-DIP) nanomicelles were combined with folic acid, the targeted effect of folated-PEG-P(GA-DIP) nanomicelles was evident in the fluorescence and MRI results. Results: To further increase the loading efficiency and the cell-uptake of encapsulated drugs (DOX and SPIONs), DIP (pK a ≈6.3) groups were linked with ∼50% of the side carboxyl groups of poly(L-glutamic acid) (PGA), to generate the core cross-linking under neutral or weakly acidic conditions. Under the acidic condition (eg, endosome/lysosome), the carboxyl groups were neutralized to facilitate disassembly of the P(GA-DIP) blocks' cross-linking, for duly accelerating the encapsulated drug release. Combined with the tumor-targeting effect of folic acid, specific drug delivery to the liver cancer cells and MRI diagnosis of these cells were greatly enhanced. Conclusion: Acid-triggered and folate-decorated nanomicelles encapsulating SPIONs and DOX, facilitate the targeted MRI diagnosis and therapeutic effects in tumors.

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Reduction-sensitive core-cross-linked mPEG–poly(ester-carbonate) micelles for glutathione-triggered intracellular drug release

Cited by 74 publications

References 45 publications

Sensitizing nanoparticle based platinum(IV) drugs by curcumin for better chemotherapy

Sensitizing nanoparticle based platinum(IV) drugs by curcumin for better chemotherapy

Preparation and Evaluation of Doxorubicin-Loaded Micelles Based on Glycyrrhetinic Acid Modified Gelatin Conjugates for Targeting Hepatocellular Carcinoma

Acid-triggered core cross-linked nanomicelles for targeted drug delivery and magnetic resonance imaging in liver cancer cells

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