Reduction of viable <i>Enterococcus faecalis</i> in human radicular dentin treated with 1% cetrimide and conventional intracanal medicaments

Mejía, Jeison B. Carbajal; Arrieta, Angela Aguilar

doi:10.1111/edt.12250

Cited by 10 publications

(3 citation statements)

References 51 publications

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“…In biofilms treated with 2% CHX, a mixed population of green, yellowish green and red-labelled cells was observed indicating that CHX is less effective against biofilm cells. Our CLSM findings are in agreement with previous studies [37,38], which showed apparently live cells after 24 h treatment with 2% CHX. The limited ability of CHX to disrupt attached biofilms is well known [39], and could be attributed to its potential fixative action [40].…”

Section: Discussionsupporting

confidence: 93%

Trans-cinnamaldehyde potently kills Enterococcus faecalis biofilm cells and prevents biofilm recovery

Ali

Cheung

Matinlinna

et al. 2020

Microbial Pathogenesis

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Enterococcus faecalis is a biofilm-forming, nosocomial pathogen that is frequently isolated from failed root canal treatments. Contemporary root canal disinfectants are ineffective in eliminating these biofilms and preventing reinfection. As a result, there is a pressing need to identify novel and safe antibiofilm molecules. The effect of short-term (5 and 15 min) and long-term (24 h) treatments of TC on the viability of E. faecalis biofilms was compared with currently used root canal disinfectants. Treatment for 15 min with TC reduced biofilm metabolic activity as effective as 1% sodium hypochlorite and 2% chlorhexidine. Treatment with TC for 24 h was significantly more effective than 2% chlorhexidine in reducing the viable cell counts of biofilms. This serendipitous effect of TC was sustained for 10 days under growthfavoring conditions. For the first time, our study highlights the strong antibacterial activity of TC against E. faecalis biofilms, and notably, its ability to prevent biofilm recovery after treatment.

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Section: Discussionsupporting

confidence: 93%

Trans-cinnamaldehyde potently kills Enterococcus faecalis biofilm cells and prevents biofilm recovery

Ali

Cheung

Matinlinna

et al. 2020

Microbial Pathogenesis

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“…The efficacy of endodontic therapy is closely tied to the efficient elimination of bacterial colonization within the pulp cavity. 36 Hence, the incorporation of antibacterial agents, intracanal irrigants, and medicines plays a pivotal role in eliminating remaining microorganisms and attaining a desirable outcome in the context of root canal treatment.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

Alhaila,

Badawi,

Elbeltagy

et al. 2024

European Journal of General Dentistry

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Objective In light of the potential drawbacks associated with certain intracanal medicaments, such as triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH2)), the introduction of herbal agents has ushered in a new era in the field of dentistry. Consequently, this study aimed to explore the impact of oleanolic acid (OA) on the viability and proliferation of dental pulp stem cells (DPSCs), comparing its effects to those of conventional intracanal medicaments, TAP and Ca(OH2). Materials and Methods DPSCs were derived from the third molars of an adult donor. Flow cytometry was utilized to do a phenotypic study on DPSCs. The methyl-thiazol tetrazolium (MTT) test was used to evaluate cellular viability. The cells were subjected to various concentrations of TAP and Ca(OH)2 (5, 2.5, 1, 0.5, and 0.25 mg/mL), in addition to OA (40, 20, 10, 5, and 2.5 µM). A cell proliferation experiment assessed the cell growth precisely at 3, 5, and 7 days. Results DPSCs were characterized by flow cytometry. The mesenchymal markers (CD73, CD90, and CD105) had a positive expression. However, the hematological markers (CD14, CD34, and CD45) showed negligible expression. A notable reduction in cellular viability was seen in cells subjected to concentrations exceeding 0.5 mg/mL of TAP and Ca(OH)2 compared to the cells that were not treated (p < 0.05). The cells treated with different concentrations of OA 2.5, 5, 10, and 20 µM did not exhibit any significant variance in cell viability compared to untreated cells (p > 0.05). Moreover, the concentrations of OA (20, 10, and 5 µM) showed high proliferation level compared to TAP and Ca(OH2) especially 5µM of OA after 7 days (p < 0.05). Conclusion Our results revealed that OA exerted significant effect on the viability and proliferation of DPSCs compared to TAP and Ca(OH2).

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“…The probiotic strain was found to colonise the oral cavity of subjects in as early as three months, and then gradually declined to extremely low levels by 12 months. Altogether, the findings show that the daily use of probiotic L. paracasei SD1 strain may be suggested in children who are at an elevated risk of caries to reduce the number of Streptococcus mutans accountable for the early phases of dental decay [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Evaluation of the Antimicrobial Activity of Probiotics Against Endodontic Pathogens

Teja¹,

Raju²,

Reddy³

et al. 2022

Cureus

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Background and aim: Despite scientific evidence that even some microorganisms may be useful, endodontic intervention has persisted to prioritize the removal of all microorganisms from the root canal system. Indeed, information regarding the significant role of probiotic microorganisms in endodontic treatment has been sparse. This study aimed to carry out an in vitro evaluation of the antimicrobial activity of probiotics against endodontic pathogens. Methods: The evaluation was carried out in three stages. In Stage 1, the agar cup well procedure was used to analyse the efficiency of probiotics microorganisms against Enterococcus faecalis bacteria and Candida albicans microorganisms in the planktonic stage. In Stage 2, a deferred antagonistic experiment was used to determine the activity of probiotic microorganisms against endodontic pathogens like E. faecalis and C. albicans in the planktonic phase. In Stage 3, biofilm phase evaluation of an intracanal probiotic microorganism carrier was done. The region of maximum inhibition was measured at the end of Stages 1 and 2. The antimicrobial activity was recognized when the dimension of the region of maximum inhibition was 10 mm or above. The colony-forming unit/millilitre was measured at the end of Stage 3.Results: There was marked antimicrobial activity of probiotic microorganisms against the pathogenic microorganisms E. faecalis as well as C. albicans in Stages 1 and 3, i.e., during the evaluation involving agar cup and evaluation at the biofilm stage. However, no antimicrobial activity of probiotic microorganisms was observed against pathogenic endodontic microorganisms in Stage 2, i.e., during evaluation involving the use of the deferred antagonistic technique.Conclusion: It can be concluded that probiotic therapy is a promising antibacterial treatment approach that should be further investigated. This study shows that probiotics can help effectively in endodontic treatment and that more in vitro as well as in vivo research is needed to fully appreciate the advantages of bacteriotherapy in the field of endodontics.

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Reduction of viable Enterococcus faecalis in human radicular dentin treated with 1% cetrimide and conventional intracanal medicaments

Cited by 10 publications

References 51 publications

Trans-cinnamaldehyde potently kills Enterococcus faecalis biofilm cells and prevents biofilm recovery

Trans-cinnamaldehyde potently kills Enterococcus faecalis biofilm cells and prevents biofilm recovery

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

An In Vitro Evaluation of the Antimicrobial Activity of Probiotics Against Endodontic Pathogens

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