Reduction of Uranium(VI) Phosphate during Growth of the Thermophilic Bacterium
            <i>Thermoterrabacterium ferrireducens</i>

Khijniak, Tatiana V.; Slobodkin, A. I.; Coker, Victoria S.; Renshaw, Joanna C.; Livens, Francis R.; Bonch-Osmolovskaya, E. A.; Birkeland, Nils Kåre; Medvedeva-Lyalikova, N. N.; Lloyd, Jonathan R.

doi:10.1128/aem.71.10.6423-6426.2005

Cited by 85 publications

(61 citation statements)

References 20 publications

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“…The presence of ningyoite was corroborated by EDS as U, P, and Ca were strongly colocalized ( Figure SI-4). This mineral has also been reported to form through the reduction of a U(VI)-phosphate solid by the Gram-positive bacterium Thermoterrabacterium ferrireducens (11). For this solid, we would expect 3 U-Ca correlations at ∼3.85 Å (26).…”

Section: Structure and Composition Of The U(iv) Speciesmentioning

confidence: 99%

“…The reduction of a U(VI)-phosphate mineral phase by the bacterium Thermoterrabacterium ferrireducens lead to the formation of a U(IV) mineral ningyoite, CaU(PO 4 ) 2 , rather than uraninite (11). Furthermore, batch experiments have demonstrated that the reduction of U(VI)-citrate complexes by Clostridium species formed stable U(IV) solution complexes (12).…”

Section: Introductionmentioning

confidence: 99%

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Non-uraninite Products of Microbial U(VI) Reduction

Bernier‐Latmani

Veeramani

Vecchia

et al. 2010

Environ. Sci. Technol.

227

286

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A promising remediation approach to mitigate subsurface uranium contamination is the stimulation of indigenous bacteria to reduce mobile U(VI) to sparingly soluble U(IV). The product of microbial uranium reduction is often reported as the mineral uraninite. Here, we show that the end products of uranium reduction by several environmentally relevant bacteria (Gram-positive and Gram-negative) and their spores include a variety of U(IV) species other than uraninite. U(IV) products were prepared in chemically variable media and characterized using transmission electron microscopy (TEM) and X-ray absorption spectroscopy (XAS) to elucidate the factors favoring/inhibiting uraninite formation and to constrain molecular structure/composition of the non-uraninite reduction products. Molecular complexes of U(IV) were found to be bound to biomass, most likely through P-containing ligands. Minor U(IV)-orthophosphates such as ningyoite [CaU(PO4)2], U2O(PO4)2, and U2(PO4)(P3O10) were observed in addition to uraninite. Although factors controlling the predominance of these species are complex, the presence of various solutes was found to generally inhibit uraninite formation. These results suggest a new paradigm for U(IV) in the subsurface, i.e., that non-uraninite U(IV) products may be found more commonly than anticipated. These findings are relevant for bioremediation strategies and underscore the need for characterizing the stability of non-uraninite U(IV) species in natural settings.

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Section: Structure and Composition Of The U(iv) Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non-uraninite Products of Microbial U(VI) Reduction

Bernier‐Latmani

Veeramani

Vecchia

et al. 2010

Environ. Sci. Technol.

227

286

View full text Add to dashboard Cite

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“…(Roh et al, 2002) and Pyrobaculum islandicum (Kashefi and Lovley, 2000)) as well as Gram-positive non-spore-forming bacteria (e.g., Thermoterrabacterium sp.) (Khijniak et al, 2005). Also, among this variety of U(VI)-reducing bacteria, a subset (e.g., Anaeromyxobacter dehalogenans (Wu et al, 2006), Geobacter metallireducens, Shewanella putrefaciens (Lovley et al, 1991) and Desulfotomaculum reducens (Tebo and Obraztsova, 1998)) have been reported to conserve energy from this reaction, and to couple it to growth.…”

Section: Introductionmentioning

confidence: 99%

U(VI) reduction by spores of Clostridium acetobutylicum

Vecchia

Veeramani

Suvorova

et al. 2010

Research in Microbiology

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Vegetative cells of Clostridium acetobutylicum are known to reduce hexavalent uranium (U(VI)). We investigated the ability of spores of this organism to drive the same reaction. We found that spores were able to remove U(VI) from solution when H 2 was provided as an electron donor and to form a U(IV) precipitate. We tested several environmental conditions and found that spent vegetative cell growth medium was required for the process. Electron microscopy showed the product of reduction to accumulate outside the exosporium. Our results point towards a novel U(VI) reduction mechanism, driven by spores, that is distinct from the thoroughly studied reactions in metal-reducing Proteobacteria.

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“…So far, there are four suggested mechanisms by which bacteria may immobilize the uranyl ion, namely; (a) biosorption, (b) bioaccumulation, (c) precipitation by reaction with inorganic ligands such as phosphate and (d) microbial reduction of soluble metal species to insoluble species (Nancharaiah et al, 2006). The fourth process has been observed in Fe(III)-reducing and sulphate-reducing bacteria (Khijniak et al, 2005). Mesophilic representatives of the genera Geobacter, Shewanella, and Desulfotomaculum are also known to couple U(VI) reduction to growth, whereas U(VI) reduction in Desulfovibrio sp.…”

Section: Introductionmentioning

confidence: 99%

“…Mesophilic representatives of the genera Geobacter, Shewanella, and Desulfotomaculum are also known to couple U(VI) reduction to growth, whereas U(VI) reduction in Desulfovibrio sp. has been shown to be mainly cometabolic with no energy derived from the reduction process (Khijniak et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Uranium(VI) reduction and removal by high performing purified anaerobic cultures from mine soil

Chabalala

Chirwa

2010

Chemosphere

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Biological uranium reduction was investigated using bacteria isolated from a uranium mine in Limpopo, South Africa. Background uranium concentration in soil from the mine was determined to be 168 mg kg -1 much higher than the typical background uranium concentration in natural soils (0.30-11.7 mg kg -1 ). Therefore it was expected that the bacteria isolated from the site were resistant to U(VI) toxicity.Preliminary studies using a non-purified consortium from the mine soil showed that

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Reduction of Uranium(VI) Phosphate during Growth of the Thermophilic Bacterium Thermoterrabacterium ferrireducens

Cited by 85 publications

References 20 publications

Non-uraninite Products of Microbial U(VI) Reduction

Non-uraninite Products of Microbial U(VI) Reduction

U(VI) reduction by spores of Clostridium acetobutylicum

Uranium(VI) reduction and removal by high performing purified anaerobic cultures from mine soil

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