2005
DOI: 10.1074/jbc.m500601200
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Reduction of Unusual Iron-Sulfur Clusters in the H2-sensing Regulatory Ni-Fe Hydrogenase from Ralstonia eutropha H16

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Cited by 47 publications
(99 citation statements)
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References 47 publications
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“…Spectra were collected maximally for a scan range of 6,950 -8,450 eV. Deadtime-corrected XAS spectra were averaged after energy calibration of each scan by using the peak at 7,112 eV in the 1 st derivative of the absorption spectrum of an Fe-foil as an energy standard (estimated accuracy Ϯ 0.1 eV) (28,29). Data were then normalized, and extended EXAFS oscillations were extracted (39).…”
Section: Methodsmentioning
confidence: 99%
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“…Spectra were collected maximally for a scan range of 6,950 -8,450 eV. Deadtime-corrected XAS spectra were averaged after energy calibration of each scan by using the peak at 7,112 eV in the 1 st derivative of the absorption spectrum of an Fe-foil as an energy standard (estimated accuracy Ϯ 0.1 eV) (28,29). Data were then normalized, and extended EXAFS oscillations were extracted (39).…”
Section: Methodsmentioning
confidence: 99%
“…K␣-fluorescence-detected XAS spectra at the Fe K-edge were collected at T ϭ 20 K using an energy-resolving 13-element Ge detector and a helium cryostat as previously described (28,29) at beamline D2 of the EMBL outstation (at HASYLAB, DESY). Harmonic rejection was achieved by detuning of the Si (111) double-crystal monochromator to 50% of its peak intensity.…”
Section: Methodsmentioning
confidence: 99%
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“…The resulting unready, inactive enzyme requires a long term reactivation with H 2 to acquire a catalytically active conformation. Two other hydrogenases in R. eutropha, the soluble, hexameric NAD ϩ -reducing hydrogenase and the cytoplasmic H 2 -sensing hydrogenase, share the exceptional ability of being quickly reactivated after exposure to oxygen, and for none of them have Ni u -A signals been detected (25,61).…”
Section: Ftir Spectroscopic Characterization Of the Mbh Protein As A mentioning
confidence: 99%
“…For this study, we select the nickel-iron regulatory hydrogenase (RH) from the 'Knallgas' bacterium Ralstonia eutropha. IR studies have shown the active site of the RH to harbour a standard set of diatomic ligands on the Fe, 17 18,19 The Ni-S state is assigned as Ni II /Fe II and is EPR-silent. 17 Proton-coupled 1-electron reduction leads to the Ni-C state, which is detectable by EPR and is assigned as Ni III /Fe II with a bridging H À (Fig.…”
mentioning
confidence: 99%