Reduction of tetrazolium salts by sulfate-reducing bacteria

Fukui, M

doi:10.1016/0378-1097(89)90101-8

Cited by 7 publications

(8 citation statements)

References 16 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we worked with CTC in biofilm and planktonic cultures under strict anaerobic conditions, and the results obtained were similar to preliminary experiments in aerobic cultures (data not shown). These data suggest that the reduction of CTC by bacteria is independent of the availability of oxygen, thus confirming the results from previous studies showing CTC reduction by a variety of anaerobes during all phases of growth (Bhupathiraju et al, 1999;Fukui & Takii 1989;Walsh et al, 1995). Seemingly, intracellular enzymes (e.g.…”

Section: Discussionsupporting

confidence: 90%

Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

2008

View full text Add to dashboard Cite

The ability of oral bacteria to enter a non-growing state is believed to be an important mechanism for survival in the starved micro-environments of the oral cavity. In this study, we examined the reactivation of nutrient-deprived cells of two oral bacteria in biofilms, Streptococcus anginosus and Lactobacillus salivarius. Non-growing cells were generated by incubation in 10 mM potassium phosphate buffer for 24 h and the results were compared to those of planktonic cultures. When both types of cells were shifted from a rich, peptone-yeast extract-glucose (PYG) medium to buffer for 24 h, dehydrogenase and esterase activity measured by the fluorescent dyes 5-cyano-2,3-ditolyl-tetrazolium chloride (CTC) and fluorescein diacetate (FDA), respectively, was absent in both species. However, the membranes of the vast majority of nutrient-deprived cells remained intact as assessed by LIVE/DEAD staining. Metabolic reactivation of the nutrient-deprived biofilm cells was not observed for at least 48 h following addition of fresh PYG medium, whereas the nongrowing planktonic cultures of the same two strains were in rapid growth in less than 2 h. At 72 h, the S. anginosus biofilm cells had recovered 78 % of the dehydrogenase activity and 61 % of the esterase activity and the biomass mm "2 had increased by 30-35 %. With L. salivarius at 72 h, the biofilms had recovered 56 % and 75 % of dehydrogenase and esterase activity, respectively. Reactivation of both species in biofilms was enhanced by removal of glucose from PYG, and S. anginosus cells were particularly responsive to yeast extract (YE) medium. The data suggest that the low reactivity of non-growing biofilm cells to the introduction of fresh nutrients may be a survival strategy employed by micro-organisms in the oral cavity.

show abstract

Section: Discussionsupporting

confidence: 90%

Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

2008

View full text Add to dashboard Cite

show abstract

“…The value of 2. for both strains dropped when the cells proceeded from exponential phase to stationary one and for a short period of starvation. In the same experiment of starvation, the ratio of respiring (formazan-forming) but not growing cells to total cells increased with starvation time (Fukui and Takii, 1989). Thus, as supposed by Hattori (1985), the parameter A for SRB may reflect physiological states of original cell populations inoculated to agar plates.…”

Section: Discussionmentioning

confidence: 62%

Kinetics of Colony Formation by Sulfate-Reducing Bacteria

Fukui

Takii

1989

Bulletin of Japanese Society of Microbial Ecology

View full text Add to dashboard Cite

The colony formation of sulfate-reducing bacteria, Desulfovibrio desulfuricans and Desulfotomaculum orientis, on agar plates well fitted the theoretical curve of the FOR model proposed by Hattori (1985) The estimates of A for both strains dropped from 5 to I day-1 with cultivation in a liquid medium or starvation in a buffer solution, while that of tr was not affected so much The estimates of h and tr for D. desulfuricans were markedly affected by incubation temperature and lactate concentration in the agar medium Both factors also affected the tr value for D orientis, however only incubation temperature affected the A value for the latter organism

show abstract

“…As previously demonstrated, INT‐reducing activity can be used as a measure of the potential respiratory activity of both aerobic and anaerobic bacterial cells, there among cells of D. desulfuricans [34,38]. In the INT reduction assays of both the S(+) and S(−) experiment the percentage of INT‐formazan accumulating (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…An INT reduction assay, modified from Fukui and Takii [34], was carried out in order to estimate the proportion of active respiring cells at different timepoints during the aeration of the cell suspensions. Samples (1.0 ml) of aerated cell suspension were transferred to Hungate culture tubes containing 4.0 ml non‐reduced anoxic sulfate‐free culture medium.…”

Section: Methodsmentioning

confidence: 99%

Effects of oxygen exposure on respiratory activities of Desulfovibrio desulfuricans strain DvO1 isolated from activated sludge

Kjeldsen¹,

Joulian²,

Ingvorsen³

2005

FEMS Microbiology Ecology

View full text Add to dashboard Cite

The present study addresses the effects of oxygen exposure on the aerobic and anaerobic respiratory activity of Desulfovibrio desulfuricans strain DvO1. This strain was isolated from the highest sulfate-reduction positive most-probable-number dilution (10(6)) of an activated sludge sample, which had been subjected to 120 h of continuous aeration. Washed cell suspensions of strain DvO1 were aerated at 50% atmospheric oxygen saturation in sulfide-free media for a period of 33 h in the presence or absence of an external electron donor (10 mM lactate). During the aeration periods, samples were removed at intervals for determination of anaerobic INT [2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride]-reducing activity, anaerobic sulfate-reducing activity, and oxygen-reducing activity. The cell suspension aerated in the absence of lactate showed negligible endogenous oxygen reduction rates and therefore did not consume oxygen during the aeration period. In contrast, the cell suspension aerated in the presence of lactate sustained significant rates of oxygen reduction during the entire 33 h aeration period. Despite this, no explicit differences in the potential INT-, oxygen-, or sulfate-reducing activities were evident between the two cell suspensions during the aeration periods. Strain DvO1 remained viable throughout the 33 h aeration periods irrespective of the presence or absence of lactate, however, the oxygen exposure resulted in a dose-dependent reversible metabolic inactivation. Notably, lactate-dependent anaerobic sulfate-reducing activity recovered quickly upon anaerobiosis, and was more oxygen tolerant than lactate-dependent oxygen-reducing activity.

show abstract

Reduction of tetrazolium salts by sulfate-reducing bacteria

Cited by 7 publications

References 16 publications

Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

Kinetics of Colony Formation by Sulfate-Reducing Bacteria

Effects of oxygen exposure on respiratory activities of Desulfovibrio desulfuricans strain DvO1 isolated from activated sludge

Contact Info

Product

Resources

About