Reduction of Severe Ischemia/Reperfusion Injury in Rat Kidney Grafts by a Soluble P-Selectin Glycoprotein Ligand1

Abstract

Our data suggest that impairment of outer medullary blood flow is crucial in I/R injury of kidney grafts with prolonged cold storage. Reduction of capillary blood flow perturbations by sPSGL protects tubular cells from severe structural damage. Blocking early selectin-mediated leukocyte adhesion may have therapeutic implications in improving the prognosis of renal transplants with severe I/R injury.

A Role for Complement in the Enhanced Susceptibility of Steatotic Livers to Ischemia and Reperfusion Injury

“…Ischemia and reperfusion of a donor organ following transplantation results in an inflammatory response that causes acute injury and may also increase graft immunogenicity and alloresponsiveness of the recipient (33,34). Steatosis significantly increases the susceptibility of livers to IRI.…”

Molecular Mediators of Liver Ischemia and Reperfusion Injury: A Brief Review

“…The principle selectin ligand is P-selectin glycoprotein ligand (PSGL-1), a 220-kDa mucin-like ligand that binds all three selectins and is located on the surface of most leukocyte subclasses (82). The blockade of P-selectin and PSGL-1 protects against IRI in liver (83-87), kidney (88,89), and small intestine (90-92) animal models, and is currently being tested in renal and liver transplant patients.…”

Donor Preconditioning with Taurine Protects Kidney Grafts from Injury After Experimental Transplantation

“…Six hours after kidney transplantation (reperfusion) blood was drawn from recipient's inferior vena cava (1 mL ϫ 2) for analysis of serum blood urea nitrogen (BUN), creatinine, lactate dehydrogenase (LDH), and aspartate aminotransferase (AST). Renal damage was assessed in tissue samples taken 6 h after transplantation [9]. For the purpose of survival evaluation, another four groups of n ϭ 9 animals underwent experiments for a survival target of 1 wk.…”

“…Thirty microscopic fields (ϫ250 magnification) per kidney section were assessed and tubular damage was graded as follows: grade 0, 1, 2, 3, and 4 is equivalent to no damage, Ͻ25%, 25%-50%, Ͼ50%-75%, and Ͼ75% tubular damage, respectively. In each section, the cortex (proximal convoluted tubules), outer medulla (mainly the straight portion of the proximal tubules and the thick ascending loops of Henle), and inner medulla (collecting ducts) were examined for morphological changes as described elsewhere [9].…”