REDUCTION OF SELENIUM OXYANIONS BY ENTEROBACTER CLOACAE STRAIN SLD1a-1: REDUCTION OF SELENATE TO SELENITE

Losi, M. E.; Frankenberger, W. T.

doi:10.1897/1551-5028(1997)016<1851:rosobe>2.3.co;2

Cited by 32 publications

(59 citation statements)

References 20 publications

(29 reference statements)

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“…The utilization of the microtiter plate allows a high throughput characterization of the molybdenum reduction process . The utilisation of resting cells in the bacterial characterization of xenobiotic degradation and metal reduction in bacteria is observed in studies of selenate reduction by Enterobacter cloacae (Losi & Frankenberger Jr. 1997) and SDS biodegradation by several bacteria (Chaturvedi & Kumar 2011). …”

Section: Identification Of Molybdenum Reducing Bacteriummentioning

confidence: 99%

Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Mansur

Gusmanizar

Roslan

et al. 2017

tlsr

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Abstrak: Satu bakteria penurun molibdenum dengan keupayaan baru untuk menyahwarna pewarna azo Kuning Metanil dilaporkan. Kondisi optimum untuk penurunan molibdenum adalah pada pH 6.3 dan pada suhu 34°C. Glukosa adalah penderma elektron yang terbaik. Keperluan-keperluan lain termasuklah kepekatan fosfat yang sempit di antara 2.5 dan 7.5 mM. Profil masa pengeluaran Mo-biru menunjukkan tempoh sela masa kira-kira 12 jam, jumlah maksimum Mo-biru dihasilkan pada kepekatan molibdat 20 mM dan pengeluaran maksimum pada masa 52 jam inkubasi. Logam-logam berat seperti merkuri, perak, tembaga dan kromium merencat penurunan sebanyak 91.9, 82.7, 45.5 dan 17.4%, masing-masing. Penyahwarnaan pewarna Kuning Metanil pada kepekatan 100 dan 150 mg/L berlaku pada hari ketiga dan hari enam inkubasi, masing-masing. Kepekatan yang lebih tinggi menghasilkan degradasi separa, dengan penyahwarnaan warna sebanyak 20% berlaku pada kepekatan 400 mg/L. Bakteria ini dikenal pasti secara separa berdasarkan analisis biokimia sebagai Bacillus sp. strain Neni-10. Spektrum penyerapan Mo-biru mencadangkan bahawa kompaun terhasil adalah fosfomolibdat terturun. Pengasingan bakteria ini, yang menunjukkan penurunan logam berat dan keupayaan penyahwarnaan pewarna, adalah diperlukan, terutamanya untuk bioremediasi.Kata kunci: Penurun Molibdenum, Molibdenum Biru, Bacillus sp., Pewarna Azo, Kuning Metanil Abstract: A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amount of Mo-blue produced at a molybdate concentration of 20 mM, and a peak production at 52 h of incubation. The heavy metals mercury, silver, copper and chromium inhibited reduction by 91.9, 82.7, 45.5 and 17.4%, respectively. A complete decolourisation of the dye Metanil Yellow at 100 and * Corresponding author: yunus.upm@gmail.com Rusnam Mansor et al. 70150 mg/L occurred at day three and day six of incubations, respectively. Higher concentrations show partial degradation, with an approximately 20% decolourisation observed at 400 mg/L. The bacterium is partially identified based on biochemical analysis as Bacillus sp. strain Neni-10. The absorption spectrum of the Mo-blue suggested the compound is a reduced phosphomolybdate. The isolation of this bacterium, which shows heavy metal reduction and dye-decolorising ability, is sought after, particularly for bioremediation.

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Section: Identification Of Molybdenum Reducing Bacteriummentioning

confidence: 99%

Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Mansur

Gusmanizar

Roslan

et al. 2017

tlsr

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“…Resting cells have been used to characterise molybdenum reduction in EC 48 with satisfactory results [13]. Resting cells have also been utilized in several bacterial heavy metal reduction researches, for example on selenate reduction [21] and on degradation of SDS [22].…”

Section: Molybdenum Reducing Bacterium Identificationmentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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“…Under this conditions, other electron acceptors such as nitrate or metal ions such as manganese or iron would be used as an alternative (Haley et al, 2012). Resting cells have been used in studying heavy metals reduction such as in selenate (Losi and Jr, 1997), vanadate (Carpentier et al, 2005), chromate (Llovera et al, 1993), reductions and xenobiotics biodegradation such as phenol (Sedighi and Vahabzadeh, 2014), amides (Raj et al, 2010), diesel (Auffret et al, 2015), SDS (Chaturvedi and Kumar, 2011), and pentachlorophenol (Steiert et al, 1987).…”

Section: Identification Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

et al. 2016

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Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 o C. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.

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REDUCTION OF SELENIUM OXYANIONS BY ENTEROBACTER CLOACAE STRAIN SLD1a-1: REDUCTION OF SELENATE TO SELENITE

Abstract: Abstract-ReductionSeO 4 useful information for optimization of a bioreactor using E. cloacae SLD1a-1 to treat Se-contaminated water.

Cited by 32 publications

References 20 publications

Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

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