Reduction of Selenium Oxyanions by Enterobacter cloacae SLD1a-1: Isolation and Growth of the Bacterium and Its Expulsion of Selenium Particles

Losi, M. E.; Frankenberger, W. T.

doi:10.1128/aem.63.8.3079-3084.1997

Cited by 226 publications

(126 citation statements)

References 29 publications

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“…As discussed in the previous section, the toxic Se oxyanions can be converted by microorganisms to elemental Se 0 which is insoluble and therefore biologically unavailable. Frankenberger and co-workers have examined the potential of this bioremediation approach with Se-contaminated water [12,32,33]. Losi and Frankenberger [32] isolated a facultative bacterium capable of removing the Se oxyanion, selenate (SeO 2− 4 ) and selenite (SeO 2− 3 ) from solution.…”

Section: Reduction Of Se-oxyanions From Watermentioning

confidence: 99%

“…Studies with washed-cell suspension experiments also showed that (i) SeO 2− 3 is a transitory intermediate in reduction of SeO 2− 4 , being produced and rapidly reduced concomitantly; and (ii) NO − 3 is also reduced concomitantly at a much higher rate than SeO 2− 4 . Losi and Frankenberger [32] indicated that SLD1a-1 uses SeO 2− 4 as an alternate electron acceptor and that the rection occurs via a membrane-associated reductase(s) followed by rapid expulsion of the Se particles. In another study, they observed that the washed-cell suspension of the bacterium removed 92% of added SeO 2− 4 (at 127 µM) from solution over (Table 2).…”

Section: Reduction Of Se-oxyanions From Watermentioning

confidence: 99%

“…Rael and Frankenberger [43] isolated Aeromonas veronii from seleniferous agricultural drainage water, which was active in volatilizing DMSe. The facultative anaerobic bacterium, Enterobacter cloacae SLD1a-1 (ATCC 700258), capable of reducing SeO 2− 4 and SeO 2− 3 to Se 0 [12,32,33], was also found to be capable of methylating SeO 2− 3 [14]. Optimum Se volatilization as DMSe occurred at a pH, temperature and EC of 6.5, 35 • C and 11 dS m −1 , respectively.…”

Section: Volatilization Of Se From Watermentioning

confidence: 99%

“…Final concentrations for controls were not significantly different from original concentrations (Duncan's multiple range test, P > 0.05). Source: Losi and Frankenberger[32].…”

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confidence: 99%

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Bioremediation of selenium‐contaminated sediments and water

Frankenberger

Arshad

2001

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Selenium (Se) is a contaminant of agricultural irrigation-drainage water in the western United States, and the cause of wildlife deaths and grotesque deformities. Some approaches in reducing the toxic Se concentrations from contaminated sediments and water have been proposed, but most of these tend to be costly or ineffective. Bioremediation through microbial transformations of toxic Se species into nontoxic forms is being considered as an effective remedial alternative. The microbial reduction of toxic oxyanions of Se (SeO(4)(2-) and SeO(3)(2-)) into insoluble Se(0) or methylation of these species to dimethylselenide (DMSe) has been accepted as a potential bioremediation strategy for cleanup of Se-contaminated water and sediments. By conducting a series of laboratory, bench-scale and field studies, we have thoroughly investigated the remedial potential of these approaches. It was observed that microorganisms, particularly Enterobacter cloacea, are very active in reduction of Se oxyanions present in irrigation drainage water, into insoluble Se(0) and, by monitoring various environmental conditions and addition of organic amendments, the process could be stimulated manifold. Similarly, the process of biomethylation of Se in soil sediments and water was found active and highly dependent on specific carbon amendments (pectin and proteins), pH, temperature, moisture, aeration and activators (cofactors). Moreover, Se biomethylation was protein/peptide-limited rather than nitrogen-, amino acid- or carbon-limited. Crude casein and its components were equally stimulatory producing a >50-fold enhancement in DMSe yield. Methionine and methyl cobalamin stimulated DMSe production by Alternaria alternata, indicating that the coenzyme may mediate the transfer of a methyl group to the Se atom. An acute toxicity test involving inhalation of DMSe by rats revealed that DMSe is nontoxic. Experiments were scaled up from laboratory studies to field plots to verify the feasibility of this bioremediation approach. Based upon the promising results of these studies, a biotechnology prototype was developed which could be applicable for cleanup of polluted sediments and water throughout the western United States.

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Section: Reduction Of Se-oxyanions From Watermentioning

confidence: 99%

Section: Reduction Of Se-oxyanions From Watermentioning

confidence: 99%

Section: Volatilization Of Se From Watermentioning

confidence: 99%

“…Final concentrations for controls were not significantly different from original concentrations (Duncan's multiple range test, P > 0.05). Source: Losi and Frankenberger[32].…”

mentioning

confidence: 99%

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Bioremediation of selenium‐contaminated sediments and water

Frankenberger

Arshad

2001

BioFactors

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“…Recently, some bacterial strains which can effectively reduce selenate into selenite or elemental selenium have been reported [2,[6][7][8]12]. They are all gram-negative bacteria.…”

Section: Flavobacterium Okeanokoites Saccharococcus Thermophilus Macrmentioning

confidence: 99%