This study was conducted to investigate the effects of the length of the light period and photosynthetic photon flux density (PPFD) in the growth chamber on the germination and growth of Eustoma grandiflorum after the wet treatment of seeds at 10°C. Seeds were sown in 200-cell trays, and then the wet treatment was applied for five weeks at 10°C under dark conditions. After this treatment, all seedlings were grown for five weeks in the growth chamber at a temperature of 27.5°C under light/dark conditions. When the average PPFD was 125 μmol・m-2 ・s-1 , the germination rate eight days after transferring the cell tray into the growth chamber was 98% or higher regardless of the length of the light period of 12 to 24 h. After raising the seedlings for five weeks, the seedlings exposed to light periods of 20 and 24 h were significantly larger than at 12 h. For the seedlings grown with Key Words:bolting, cut flower characteristic forms, flower budding, flowering, rosette キーワード:抽苔,発蕾,開花,切り花形質,ロゼット