Reduction of Placental Nitric Oxide Synthase Activity in Pre-Eclampsia

Brennecke, Shaun; Gude, Neil M; Iulio, Juliana L Di; King, Roger G

doi:10.1042/cs0930051

Cited by 54 publications

(25 citation statements)

References 30 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, it has been proposed that the elevation of circulating lipid peroxides led both to peroxidative damage in the vascular endothelial cell membrane and to a decrease in the synthesis of NO [1,6]. Indeed, it was recently demonstrated that human placental NO synthase activity is significantly reduced in preeclampsia [14]. In addition, it was found that serum nitrate/nitrite levels in women with preeclampsia are lower than in control women [8].…”

Section: Discussionmentioning

confidence: 96%

Plasma Nitric Oxide Metabolites and Lipid Peroxide Levels in Preeclamptic Pregnant Women before and after Delivery

Mutlu-Türkoglu¹,

Aykaç‐Toker²,

Ibrahimoglu

et al. 1999

Gynecol Obstet Invest

View full text Add to dashboard Cite

It has been suggested that oxidative stress may cause endothelial dysfunction and that endothelial dysfunction may lead to hypertension by reduced release of vasodilating agents such as nitric oxide (NO). In this study, we investigated the relationship between serum NO and lipid peroxides in preeclamptic and normal pregnant women before and after delivery. Plasma from women with preeclampsia had significantly lower nitrate/nitrite concentrations and significantly higher lipid peroxide levels than normal pregnant women before the delivery. Lipid peroxide levels were significantly elevated in preeclamptic placenta. After delivery in the preeclamptic group the plasma concentration of nitrate/nitrite was increased and plasma thiobarbituric acid reactive substance levels decreased, while these parameters remained unchanged in the normal pregnants women. These results indicate that high levels of lipid peroxides in the circulation may be the cause of lowered NO synthesis and hypertension observed in preeclamptic women.

show abstract

Section: Discussionmentioning

confidence: 96%

Plasma Nitric Oxide Metabolites and Lipid Peroxide Levels in Preeclamptic Pregnant Women before and after Delivery

Mutlu-Türkoglu¹,

Aykaç‐Toker²,

Ibrahimoglu

et al. 1999

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

“…Placental production of some vasodilator autacoids such as prostacyclin [14] and nitric oxide [7] has been shown to be decreased during pre-eclampsia, and these decreases are thought to play a role in increased vascular resistance and sensitivity to vasoconstrictor agents in the placenta in that disorder. As the relationship between pre-eclampsia and placental ANF is not known, the present study investigated the overall production of ANF by the placenta during pre-eclampsia by measuring pro-ANF mRNA levels in placentae obtained from normotensive pregnancies and pregnancies complicated by pre-eclampsia using Northern blot analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Increased placental production of ANF may contribute to compensatory functions in the fetal and/or maternal circulations. On the other hand, reduced -hypertensive medication 37 1 1 2,150 !10 hydralazine, labetalol, nifedipine 2 37 2 2 3,310 77 nifedipine 3 37 5 3 3,235 72 methyldopa 4 37 6 2 3,150 66 methyldopa 5 37 2 2 2,100 !10 6 37 3 3 2,285 !10 7 37 1 1 2,535 26 8 38 1 1 3,270 61 9 32 1 1 1,635 42 hydralazine 10 30 1 1 1,185 27 methyldopa 11 33 1 placental production of ANF may contribute to the endocrine imbalance towards increased vasoconstriction that is known to occur in the placenta during pre-eclampsia [1,7]. In order to assess the placental production of ANF during pre-eclampsia, we have examined and compared the abundance of pro-ANF mRNA in placental tissue collected at caesarean section from women with pre-eclampsia or normotensive pregnancies, who were delivered at term or pre-term gestations.…”

Section: Introductionmentioning

confidence: 99%

Relative Abundance of Placental Pro-Atrial Natriuretic Factor mRNA in Normal Pregnancy and Pre-Eclampsia

Gude

Wang

Xue

et al. 2000

Gynecol Obstet Invest

View full text Add to dashboard Cite

Atrial natriuretic factor (ANF), produced by cytotrophoblast cells of the human placenta, may be involved in the regulation of uteroplacental blood flow. Pre-eclampsia is associated with maternal hypertension and reduced uteroplacental perfusion. The relationship between pre-eclampsia and placental production of ANF is not known. This study measured pro-ANF mRNA levels by Northern blot analysis in placentae delivered by caesarean section at preterm and term gestations from women with normotensive and pre-eclamptic pregnancies and found no significant difference between pre-eclampsia and normal pregnancy at either gestation. This result suggests that placental production of ANF is not altered at the pretranslational level during pre-eclampsia.

show abstract

“…Studies screening for NOS mRNA species and protein expression in normalterm placentae have demonstrated the presence of eNOS, and NOS activity assays show that the majority of placental NOS activity is calcium-dependent [6][7][8][9]. In addition, there is also evidence to suggest that in pathological states such as pre-eclampsia, eNOS is the predominant isoform [10]. In normal placentae, eNOS has been localized to the syncytiotrophoblast cell layer and the villous endothelium [6,8].…”

Section: Introductionmentioning

confidence: 99%

Human placental nitric oxide synthase activity is not altered in diabetes

Iulio¹,

Gude²,

King³

et al. 1999

Clinical Science

View full text Add to dashboard Cite

Endothelial nitric oxide synthase (NOS) protein and mRNA have been identified and calcium-dependent NOS activity has been measured in human placentae during normal pregnancy. Recently, mRNA and protein for the inducible isoform of NOS have been detected in placentae of women with gestational diabetes. The aim of this study was to determine whether calcium-independent (ciNOS) and/or total (tNOS) NOS activities were increased in placentae obtained after vaginal delivery or Caesarean section from women assigned to the following groups according to standard obstetric criteria: gestational diabetes, diabetes before pregnancy and non-diabetic controls. tNOS and ciNOS were assessed by measuring the conversion of [3H]L-arginine to [3H]L-citrulline in the three groups. Michaelis-Menten constants (Km) and maximum velocities of reaction (Vmax) were calculated using Lineweaver-Burk analysis for tNOS. There were no significant differences in either ciNOS, Vmax or Km values between any of the three groups (normal, ciNOS 12.7+/-1.6%, Vmax 16.6+/-3.3 pmol.min-1.mg-1 protein, Km 15.30+/-2.6 micromol/l; gestational diabetes, ciNOS 15.4+/-1.4%, Vmax 14.8+/-5.2 pmol.min-1. mg-1 protein, Km 10.5+/-1.7 micromol/l; diabetes before pregnancy, ciNOS 13.4+/-1.1%, Vmax 14.9+/-3.4 pmol.min-1.mg-1 protein, Km 17. 7+/-2.2 micromol/l). The presence of macrosomia did not affect tNOS activity in those with diabetes before pregnancy, and glycosylated haemoglobin levels measured between weeks 27 and 39 were not correlated with ciNOS activity. The results from the present study do not provide evidence for increased placental tNOS or ciNOS activities in pregnancies complicated by gestational diabetes or diabetes present before pregnancy.

show abstract

Reduction of Placental Nitric Oxide Synthase Activity in Pre-Eclampsia

Cited by 54 publications

References 30 publications

Plasma Nitric Oxide Metabolites and Lipid Peroxide Levels in Preeclamptic Pregnant Women before and after Delivery

Plasma Nitric Oxide Metabolites and Lipid Peroxide Levels in Preeclamptic Pregnant Women before and after Delivery

Relative Abundance of Placental Pro-Atrial Natriuretic Factor mRNA in Normal Pregnancy and Pre-Eclampsia

Human placental nitric oxide synthase activity is not altered in diabetes

Contact Info

Product

Resources

About