Reduction of oxidative stress in adjuvant arthritis. Comparison of efficacy of two pyridoindoles: stobadine dipalmitate and SMe1.2HCl.

Poništ, Silvester; Mihalová, Danica; Jančinová, Viera; Snirc, Vladimir; Ondrejicková, O; Mascia, Cinzia; Poli, Giuseppe; Stancíková, M; Nosáľ, R; Bauerová, Katarína

doi:10.18388/abp.2010_2398

Cited by 11 publications

(8 citation statements)

References 36 publications

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“…TNF-α blockers may be used alternatively with other candidates for RA combination therapy. As resulted from our previous experiments (Bauerova et al ., 2005a ; 2005b ; 2008a ; 2008b ; 2009 ; Drábikova et al ., 2009 ; Dráfi et al ., 2010 ; Jančinova et al ., 2009 ; Kogan et al ., 2005 ; Mačičkova et al ., 2010 ; Poništ et al ., 2010 ; Sotnikova et al ., 2009 ; Štrosova et al ., 2008 ; 2009 ), all performed in the adjuvant arthritis model, substances with antioxidant properties have a high potency to be used in combination therapy with Mtx.…”

Section: Discussionmentioning

confidence: 99%

“…We assume that the increased activity of GGT in AA is a result of elevated systemic oxidative stress. Stb was significantly effective in suppressing the increased activity of GGT in the joint, as expected from its antioxidant potential (Poništ et al ., 2010 ). The addition of Stb to Mtx caused significant improvement in lowering this biochemical parameter compared to Mtx monotherapy ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…In our experiments of the last five years, synthetic (pyridoindoles) and natural substances (polysaccharides: glucomannan, beta-glucan; endogenous molecules: coenzyme Q 10 and carnosine; different substances and products of plant origin) with immunomodulatory and antioxidative effects were assessed in monotherapy by using experimental adjuvant arthritis (AA). These active substances decreased the progression of AA when administered to arthritic rats over the period of 28 days (Bauerova et al ., 2005a ; 2005b ; 2008a ; 2008b ; 2009 ; Drabikova et al ., 2009 ; Drafi et al ., 2010 ; Jančinova et al ., 2009 ; Kogan et al ., 2005 ; Mačičkova et al ., 2010 ; Poništ et al ., 2010 ; Sotnikova et al ., 2009 ; Štrosova et al ., 2008 , 2009 ). Recently we focused on combined therapy with Mtx and confirmed the potent effect of this drug in several experiments administered along with an appropriate antioxidative substance (Bauerova et al ., 2010 ; Jančinova et al ., 2010 ; Rovensky et al ., 2009a ).…”

Section: Introductionmentioning

confidence: 99%

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Utilization of adjuvant arthritis model for evaluation of new approaches in rheumatoid arthritis therapy focused on regulation of immune processes and oxidative stress

Bauerová¹,

Poništ²,

Mihalová³

et al. 2011

Interdisciplinary Toxicology

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As a number of disease-modifying anti-rheumatic drugs often have side effects at high doses and/or during long-term administration, increased efficacy without increased toxicity is expected for combination therapy of rheumatoid arthritis (RA). The safety of long-term therapy of RA is very important as patients with RA are usually treated for two or more decades. This experimental overview is focused on some promising substances and their combinations with the standard antirheumatic drug – methotrexate (Mtx) for treatment of rheumatoid arthritis. The adjuvant arthritis model in Lewis rats was used for evaluation of antiinflammatory efficacy of the substances evaluated. Mtx was administered in the oral dose of 0.3 mg/kg b.w. twice a week. Natural and synthetic antioxidants were administered in the daily oral dose of 20 mg/kg b.w for coenzyme Q10 (CoQ10), 150 mg/kg b.w for carnosine (Carn), 15 mg/kg b.w. for stobadine dipalmitate (Stb) and its derivative SMe1.2HCl (SMe1), and 30 mg/kg b.w. for pinosylvin (Pin) or pterostilbene (Pte). Mtx in the oral dose of 0.4 mg/kg b.w. twice a week was combined with Pin in the oral daily dose of 50 mg/kg b.w. Clinical (hind paw volume – HPV), biochemical (activity of GGT in joint and level of TBARS in plasma), and immunological (IL-1 in plasma) parameters were assessed. Our results achieved with different antioxidants in monotherapies showed a reduction of oxidative stress in adjuvant arthritis independently of the chemical structure of the compounds. Pin was the most effective antioxidant tested in decreasing HPV. All combinations tested showed a higher efficacy in affecting biochemical or immunological parameters than Mtx administered in monotherapy. The findings showed the benefit of antioxidant compounds for their use in combination therapy with methotrexate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Utilization of adjuvant arthritis model for evaluation of new approaches in rheumatoid arthritis therapy focused on regulation of immune processes and oxidative stress

Bauerová¹,

Poništ²,

Mihalová³

et al. 2011

Interdisciplinary Toxicology

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“…Dietary studies (diet high in polyunsaturated fatty acids) in six female RA patients have reported significantly decreased neutrophil chemiluminescence . An animal study found significantly elevated whole blood chemiluminescence in adjuvant arthritis when compared with healthy control animals; which could be significantly reduced by antioxidants …”

Section: Introductionmentioning

confidence: 99%

Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson's disease patients

2018

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The consequences of oxidative stress and inflammation are implicated in a wide range of diseases, including rheumatoid arthritis and Parkinson's disease. The status of antioxidant capacity in rheumatoid arthritis and Parkinson's disease remains unclear, in part due to common practice of assaying erythrocytes separately to plasma. This method removes any synergistic interactions between plasma and erythrocyte-based antioxidants. The experiments in this report tested antioxidant capacity in whole blood, erythrocytes and plasma by group and disease stage. Medically diagnosed patients were recruited along with appropriate control group participants. Fasting venous blood was assayed using chemiluminescence methods for: time to maximum light emitted, maximum light emitted, and plasma antioxidant capacity in vitamin E analogue units. Here we demonstrate that whole blood exhibits higher antioxidant capacity than either plasma or erythrocytes assayed separately. We report increased oxidative stress in the blood of rheumatoid arthritis patients by group (p = 0.018, p = 0.049). We show increased antioxidant capacity in Parkinson's disease patients by group (p < 0.001). For later stage Parkinson's disease patients, we report reduced oxidative stress (p = 0.025), and increased antioxidant capacity and for erythrocytes (p < 0.001, p = 0.004) and whole blood (p < 0.001, p = 0.003). Early stage Parkinson's disease showed higher antioxidant capacity on only one measure (p = 0.008). Whole blood chemiluminescence is a useful technique for determining redox status in disease and might help clarify the role of oxidative stress in rheumatoid arthritis and Parkinson's disease.

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“…To study the influence of neutrophils on pathogenesis of rheumatoid arthritis and to investigate the effects of new compounds on this process, different animal models of arthritis are used. One of them is the model of adjuvant arthritis induced by heat-inactivated Mycobacterium butyricum in Freund's adjuvant [13]. Mohr et al showed that under normal conditions, there were no neutrophils in the articular capsule of nonarthritic animals, while the inflamed synovial and capsular tissue of adjuvant injected animals is heavily infiltrated with neutrophils [7].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Pterostilbene on Neutrophil Activity in Experimental Model of Arthritis

Perečko

Drábiková

Lojek

et al. 2013

BioMed Research International

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It has been demonstrated that pterostilbene inhibits reactive oxygen species production in neutrophils in vitro. However, little is known about its effects on neutrophils during inflammation in vivo. In this study, the effect of pterostilbene on neutrophil activity was investigated in experimental arthritis model. Lewis rats were injected by a single intradermal injection of heat-killed Mycobacterium butyricum in Freund's adjuvant to develop arthritis. Another group of arthritic animals received pterostilbene 30 mg/kg, daily, p.o. The number and activity of neutrophils in blood were measured on a weekly basis during the whole experiment. Moreover, the total radical trapping potential in plasma was measured at the end of the experiment. In the pterostilbene treated arthritic group, the treatment significantly lowered the number of neutrophils in blood on days 14 and 21 without significant downregulation of neutrophil oxidative burst. Pterostilbene nonsignificantly increased total radical trapping potential in arthritic animals. These results indicate that the promising effects of pterostilbene on reactive oxygen species operate by different mechanisms in vitro and in the animal model of inflammation. In conclusion, the positive effects of pterostilbene in the model of arthritis may be attributed to regulation of neutrophil number.

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Reduction of oxidative stress in adjuvant arthritis. Comparison of efficacy of two pyridoindoles: stobadine dipalmitate and SMe1.2HCl.

Cited by 11 publications

References 36 publications

Utilization of adjuvant arthritis model for evaluation of new approaches in rheumatoid arthritis therapy focused on regulation of immune processes and oxidative stress

Utilization of adjuvant arthritis model for evaluation of new approaches in rheumatoid arthritis therapy focused on regulation of immune processes and oxidative stress

Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson's disease patients

The Effects of Pterostilbene on Neutrophil Activity in Experimental Model of Arthritis

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