Reduction of miR‐212 contributes to pituitary adenoma cell invasion via targeting c‐Met

Wang, Hongyan; Gao, Huai-Qing

doi:10.1002/kjm2.12137

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…Up-regulating the target of miR-137 significantly inhibits the proliferation and migration of IPA cells (5). Meanwhile, other studies have also pointed out that a variety of miRNAs are differentially expressed in IPA tissues or cells, including miR-16 (6), miR-106b (7), miR-212 (8), miR-133 (9), and miR-410 (10). It has been found that the expression of miR-183 is imbalanced in a variety of nervous system tumor tissues or cells, which affects the invasion and migration ability of cancer cells (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Caveolin-1 regulates the expression of miR-183 and inhibits the invasion and migration of invasive pituitary adenomas by affecting early growth response 1 (EGR1)/Krueppel-like factor 5 (KLF5) interaction

Ma¹,

Zhang²,

Wang³

et al. 2020

Gland Surg

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Background: This study aims to investigate the mechanism through which Caveolin-1 (CAV-1) regulates the expression of micro ribonucleic acid (miR)-183 in invasive pituitary adenoma (IPA) tissues and GH3 cells, and explore the effects of CAV-1 and miR-183 on the invasion and migration ability of GH3 cells.Methods: Western blotting was used to detect the expression level of CAV-1, early growth response 1 (EGR1) and Krueppel-like factor 5 (KLF5). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-183. The mechanisms of interaction between CAV-1, EGR1, and KLF5 were studied by immunoprecipitation experiments. Transwell and cell scratch tests were used to determine the invasion and migration ability of GH3 cells. The dual-luciferase reporter gene experiment was used to detect the effects of EGR1 and KLF5 on miR-183 luciferase activity and verify the targeting relationship between miR-183 and ezrin. Results:The expression of CAV-1 was up-regulated. However, following the knockdown of CAV-1, the invasion and migration ability of GH3 cells was significantly inhibited (P<0.05). The expression of miR-183 was down-regulated, but the expression level of miR-183 was markedly increased following the knockdown of CAV-1 (P<0.05). The knockdown of CAV-1 inhibited the nuclear ectopic of the EGR1 protein in GH3 cells.At the same time, the interaction between EGR1 and KLF5 in GH3 cells was significantly inhibited (P<0.05).The luciferase activity of miR-183 increased significantly after overexpression of KLF5 while overexpression of EGR1 and KLF5 had no significant effect on intracellular luciferase activity. Overexpression of miR-183 markedly inhibited the luciferase activity of wild-type EZR and the expression of the EZR protein in GH3 cells. Furthermore, the overexpression of miR-183 or the inhibition of EZR can reduce the invasion and migration ability of GH3 cells. The simultaneous overexpression or inhibition of miR-183 and EZR expression has no obvious effect on the invasion and migration ability of GH3 cells.Conclusions: CAV-1 up-regulates the expression of miR-183 by inhibiting the nuclear ectopic of EGR1 and the interaction between EGR1 and KLF5 in GH3 cells. Also, miR-183 negatively regulates the expression of EZR and inhibits the invasion and migration of GH3 cells.

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Section: Introductionmentioning

confidence: 99%

Caveolin-1 regulates the expression of miR-183 and inhibits the invasion and migration of invasive pituitary adenomas by affecting early growth response 1 (EGR1)/Krueppel-like factor 5 (KLF5) interaction

Ma¹,

Zhang²,

Wang³

et al. 2020

Gland Surg

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Non-coding RNAs and exosomal non-coding RNAs in pituitary adenoma

Rahimian,

Sheida,

Rajabi

et al. 2023

Pathology - Research and Practice

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The Role of Noncoding RNAs in Pituitary Adenoma

et al. 2021

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Pituitary adenomas (PAs) are common cranial tumors that affect the quality of life in patients. Early detection of PA is beneficial for avoiding clinical complications of this disease and increasing the quality of life. Noncoding RNAs, including long noncoding RNA, miRNA and circRNA, regulate protein expression, mostly by inhibiting the translation process. Studies have shown that dysregulation of noncoding RNAs is associated with PA. Hence understanding the expression pattern of noncoding RNAs can be considered a promising method for developing biomarkers. This article reviews data on the expression pattern of dysregulated noncoding RNAs involved in PA. Possible molecular mechanisms by which the dysregulated noncoding RNA could possibly induce PA are also described.

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Reduction of miR‐212 contributes to pituitary adenoma cell invasion via targeting c‐Met

Cited by 3 publications

References 27 publications

Caveolin-1 regulates the expression of miR-183 and inhibits the invasion and migration of invasive pituitary adenomas by affecting early growth response 1 (EGR1)/Krueppel-like factor 5 (KLF5) interaction

Caveolin-1 regulates the expression of miR-183 and inhibits the invasion and migration of invasive pituitary adenomas by affecting early growth response 1 (EGR1)/Krueppel-like factor 5 (KLF5) interaction

Non-coding RNAs and exosomal non-coding RNAs in pituitary adenoma

The Role of Noncoding RNAs in Pituitary Adenoma

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