Reduction of long-chain fatty acid ceramides in dermatitis caused by repeated exposure to house-dust mite in NC/Nga mice

Kanoh, Hiroyuki; Ishitsuka, Asako; Fujine, Etsuko; Nakamura, Mitsuhiro; Matsuhaba, Shuhei; Inagaki, Nobuya; Banno, Yoshiko; Seishima, Mariko

doi:10.1016/j.jdermsci.2017.02.043

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“…Quantification of lipids using liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed a significant increase in production of total ceramide after treatment with ASC-exosomes ( Figure 5A). While ceramides are composed of a sphingoid base (sphingosine) linked to different chain lengths of fatty acid, a significant decrease in long-chain ceramides (above C20:0) in AD skin has been highlighted in previous studies [52][53][54]. Therefore, we further clarified the change in long chain ceramides, and our LC-MS/MS analysis revealed that ASC-exosomes selectively increase the number of ceramides carrying long acyl chains, C20:0, C22:0, C24:0, and C24:1 (Figure 5B-E).…”

Section: Asc-exosome-mediated Improvements In Epidermal Barrier Functmentioning

confidence: 88%

Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis

Shin

Ha²,

Kim³

et al. 2020

Cells

121

127

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Atopic dermatitis (AD) is a multifactorial, heterogeneous disease associated with epidermal barrier disruption and intense systemic inflammation. Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. Here, we investigated ASC-exosomes’ effects on skin barrier restoration by analyzing protein and lipid contents. We found that subcutaneous injection of ASC-exosomes in an oxazolone-induced dermatitis model remarkably reduced trans-epidermal water loss, while enhancing stratum corneum (SC) hydration and markedly decreasing the levels of inflammatory cytokines such as IL-4, IL-5, IL-13, TNF-α, IFN-γ, IL-17, and TSLP, all in a dose-dependent manner. Interestingly, ASC-exosomes induced the production of ceramides and dihydroceramides. Electron microscopic analysis revealed enhanced epidermal lamellar bodies and formation of lamellar layer at the interface of the SC and stratum granulosum with ASC-exosomes treatment. Deep RNA sequencing analysis of skin lesions demonstrated that ASC-exosomes restores the expression of genes involved in skin barrier, lipid metabolism, cell cycle, and inflammatory response in the diseased area. Collectively, our results suggest that ASC-exosomes effectively restore epidermal barrier functions in AD by facilitating the de novo synthesis of ceramides, resulting in a promising cell-free therapeutic option for treating AD.

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Section: Asc-exosome-mediated Improvements In Epidermal Barrier Functmentioning

confidence: 88%

Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis

Shin

Ha²,

Kim³

et al. 2020

Cells

121

127

View full text Add to dashboard Cite

show abstract

Reduction of long-chain fatty acid ceramides in dermatitis caused by repeated exposure to house-dust mite in NC/Nga mice

Cited by 1 publication

References 0 publications

Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis

Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis

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