Reduction of hexavalent chromium by Pseudomonas fluorescens LB300 in batch and continuous cultures

DeLeo, Paul C.; Ehrlich, Henry L.

doi:10.1007/bf00173341

Cited by 107 publications

(31 citation statements)

References 9 publications

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“…McLean and Beveridge (2001) have reported that Arrow indicated intracellular accumulation of chromium Pseudomonad (CRB5) was reduced toxic chromate to insoluble Cr (III) precipitation under aerobic and anaerobic condition. De Leo and Ehrlich (1994) reported that formation of Cr(III) precipitates was not evident in batch and continuous cultures of P. fluorescens LB300 whereas Pseudomonas chromatophillica, P. ambigna and Pseudomonas maltophilia all induce the formation of precipitates due to accumulation of metal. Microorganisms have excellent nucleation sites for grained mineral formation, due to their high surface area and volume ratio and the presence of electronegative charges on the cell wall (Beveridge 1988).…”

Section: Discussionmentioning

confidence: 98%

Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent

2007

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Two bacterial consortia were developed by continuous enrichment of microbial population of tannery and pulp and paper mill effluent contained Serratia mercascens, Pseudomonas fluorescence, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter sp. identified by 16S rDNA method. The consortia evaluated for removal of chromate [(Cr(VI)] in shake flask culture indicated pulp and paper mill consortium had more potential for removal of chromate. Acinetobacter sp. isolated from pulp and paper mill consortium removed higher amount of chromate [Cr(VI)] under aerobic conditions. Parameters optimized in different carbon, nitrogen sources, and pH, indicated maximum removal of chromate in sodium acetate (0.2%), sodium nitrate (0.1%) and pH 7 by Acinetobacter sp. Bacteria was applied in 2-l bioreactor significantly removed chromate after 3 days. The results of the study indicated removal of more than 75% chromium by Acinetobacter sp. determined by diphenylcarbazide colorimetric assay and atomic absorption spectrophotometer after 7 days. Study of microbial [Cr(VI)] removal and identification of reduction intermediates has been hindered by the lack of analytical techniques. Therefore, removal of chromium was further substantiated by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) which indicated bioaccumulation of chromium in the bacterial cells.

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Section: Discussionmentioning

confidence: 98%

Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent

2007

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“…DeLeo and Ehrlich [6] medium (grams per litre: tryptone 10, yeast extract 5, NaCl 5, citric acid 1, Na 2 HPO 4 6.9) was used for these experiments. Cultures were grown at 37°C at 150 rpm.…”

Section: Cr(vi) Reduction Experimentsmentioning

confidence: 99%

“…Cultures were grown at 37°C at 150 rpm. Periodically (24,48,72 and 96 h), samples were drawn aseptically and analysed for Cr(VI) reduction following the procedure of DeLeo and Ehrlich [6]. To monitor Cr(VI) reduction in cyanobacteria, BG 11 medium at two initial K 2 CrO 4 concentrations (100 and 200 mg mL À1 ) was used.…”

Section: Cr(vi) Reduction Experimentsmentioning

confidence: 99%

Chromium-resistant bacteria and cyanobacteria: impact on Cr(VI) reduction potential and plant growth

Faisal

Hameed

Hasnain

2005

J IND MICROBIOL BIOTECHNOL

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Two chromium-resistant bacterial strains, Bacillus cereus S-6 and Ochrobactrum intermedium CrT-1, and two cyanobacterial strains, Oscillatoria sp. and Synechocystis sp., were used in this study. At initial chromate concentrations of 300 and 600 microg K2CrO4 mL(-1), and an inoculum size of 9.6 x 10(7) cells mL(-1), B. cereus S-6 completely reduced Cr(VI), while O. intermedium CrT-1 reduced Cr(VI) by 98% and 70%, respectively after 96 h. At 100 microg K2CrO4 mL(-1), Synechocystis sp. MK(S) and Oscillatoria sp. BJ2 reduced 62.1% and 39.9% of Cr(VI), respectively, at 30 degrees C and pH 8. Application of hexavalent chromate salts adversely affected wheat seedling growth and anatomical characters. However, bacterial inoculation alleviated the toxic effects, as reflected by significant improvements in growth as well as anatomical parameters. Cyanobacterial strains also led to some enhancement of various growth parameters in wheat seedlings.

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“…Cadmium and Pb, though non-essential and non-beneficial, are considered highly toxic to plants, animals and microbes (Ajmal et al 1998). Chromium(VI) species are also extremely toxic and exhibit mutagenic and carcinogenic effects on biological systems (DeLeo & Ehrlich 1994;McLean & Beveridge 2001). Microbial reduction of Cr(VI)) to Cr(III) is a potentially useful process for remediation of Cr(VI)-affected environments (Michel et al 2001;Ganguli & Tripathi 2002;Camargo et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

Raja

Anbazhagan

Selvam

2006

World J Microbiol Biotechnol

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The use of microorganisms to remove heavy metals from industrial effluent is an area of extensive research and development. Attempts have been made to isolate and characterize metal-resistant microorganisms from treated oil mill industry effluent wastewater samples. The metal-resistant organisms that showed values of minimum inhibitory concentration towards metals (Cd, Cr, Ni and Pb) ranging from 100 to 800 ppm level were screened. A potent metal-resistant organism, isolate BC15 from the wastewater samples was tentatively identified as Pseudomonas sp. Detailed analysis of morphological, biochemical and 16S rDNA sequence of the isolate revealed that it is closely related to Pseudomonas aeruginosa (94%). Pseudomonas BC15 was capable of absorbing 93% Ni, 65% Pb, 50% Cd and 30% Cr within 48 h from the medium containing 100 mg of each heavy metal per liter. The multiple metal tolerance of this strain was also associated with resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol, erythromycin, kanamycin and streptomycin.

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Reduction of hexavalent chromium by Pseudomonas fluorescens LB300 in batch and continuous cultures

Cited by 107 publications

References 9 publications

Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent

Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent

Chromium-resistant bacteria and cyanobacteria: impact on Cr(VI) reduction potential and plant growth

Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

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