Reduction of Different Nitro-Compounds on Electroenzymatic System: Cytochrome c Reductase-NAD(P)H Modified Carbon Paste Electrode

Tijoux, María Emilia; Núñez‐Vergara, Luis J.; Squella, J.A.

doi:10.1002/(sici)1521-4109(199911)11:17<1285::aid-elan1285>3.0.co;2-t

Cited by 6 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed highest reduction potential for NB was −0.356 V in the abiotic cathode, which was lower than the −0.15∼−0.25 V reported by Mu et al in a BES using graphite granules with higher surface area . After the formation of biocathode, highest reduction potential was positively shifted from −0.356 V to −0.201 V. The increase of peak current and the positive shift of reduction potential are typical electrochemical indications for improved catalysis , and were consistent with the electroenzymatic system for nitro-compounds reduction . The reduction of nitroaromatics is usually catalyzed by a nitroreductase in bacteria .…”

Section: Resultssupporting

confidence: 78%

“…11 After the formation of biocathode, highest reduction potential was positively shifted from À0.356 V to À0.201 V. The increase of peak current and the positive shift of reduction potential are typical electrochemical indications for improved catalysis 22,23 and were consistent with the electroenzymatic system for nitro-compounds reduction. 24 The reduction of nitroaromatics is usually catalyzed by a nitroreductase in bacteria. 6 Flavin mononucleotide (FMN) was found as the functional group in many bacterial nitroreductases.…”

Section: ' Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode

Wang

Cheng

Liang

et al. 2011

Environ. Sci. Technol.

268

153

View full text Add to dashboard Cite

Nitrobenzene (NB) is a toxic compound that is often found as a pollutant in the environment. The present removal strategies suffer from high cost or slow conversion rate. Here, we investigated the conversion of NB to aniline (AN), a less toxic endproduct that can easily be mineralized, using a fed-batch bioelectrochemical system with microbially catalyzed cathode. When a voltage of 0.5 V was applied in the presence of glucose, 88.2 ± 0.60% of the supplied NB (0.5 mM) was transformed to AN within 24 h, which was 10.25 and 2.90 times higher than an abiotic cathode and open circuit controlled experiment, respectively. AN was the only product detected during bioelectrochemical reduction of NB (maximum efficiency 98.70 ± 0.87%), whereas in abiotic conditions nitrosobenzene was observed as intermediate of NB reduction to AN (decreased efficiency to 73.75 ± 3.2%). When glucose was replaced by NaHCO(3), the rate of NB degradation decreased about 10%, selective transformation of NB to AN was still achieved (98.93 ± 0.77%). Upon autoclaving the cathode electrode, nitrosobenzene was formed as an intermediate, leading to a decreased AN formation efficiency of 71.6%. Cyclic voltammetry highlighted higher peak currents as well as decreased overpotentials for NB reduction at the biocathode. 16S rRNA based analysis of the biofilm on the cathode indicated that the cathode was dominated by an Enterococcus species closely related to Enterococcus aquimarinus.

show abstract

Section: Resultssupporting

confidence: 78%

Section: ' Materials and Methodsmentioning

confidence: 99%

Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode

Wang

Cheng

Liang

et al. 2011

Environ. Sci. Technol.

268

153

View full text Add to dashboard Cite

show abstract

“…Recently, Pires et al 5 found that the nitroheterocyclics cytotoxicity depends on the chemical nitro group reduction, which subsequently results in damage to DNA; however, the precise action mechanism is not yet completely understood. Merino et al 6 proposed a biological action mechanism for these drugs in which the nitro group reduction produced intermediate species that interact with DNA by oxidizing it and liberating thymidine phosphate, which causes damage to DNA by destabilizing the double helix 7. Moreover, the effect of the substituents at position 2 in these nitroheterocyclic compounds has been studied over a wide range of molecules, and the active compounds have been classified into the azomethine (CHN), vinyl (CHCH), and heterocyclic groups.…”

Section: Introductionmentioning

confidence: 99%

Electronic and physicochemical properties of selected nitrofurans: A theoretical study

Soriano-Correa¹,

Raya

Sánchez-Ruiz³

et al. 2005

Int J of Quantum Chemistry

View full text Add to dashboard Cite

ABSTRACT:A relationship between the chemical structure and antibacterial activity of nitrofurans is not still completely elucidated; however, from a therapeutics point of view it has been observed that the position of the nitro group is a relevant feature for them to show antibacterial activity. In this work, we performed an ab initio study of some selected nitrofurans at the Hartree-Fock, second-order Møller-Plesset, and Density Functional levels of theory with the purpose of obtaining several electronic structural and physicochemical properties of nitrofurans. The relative acidity of the azomethine group of nitrofurans was analyzed through the deprotonation energy. The results reveal that the less acidic molecules possess antibacterial activity. The bond order analysis suggests that the activity of the nitrofurans could be affected by the electroattracting properties of the substituent. An atomic charge and electrostatic potential analysis showed that the azomethine group, the nitro group, and the furenic ring are influenced by the substituent electronegativity. Finally, a structural analysis showed that the nitro group is coplanar with respect to the furenic ring. The present study reveals that the analyzed physicochemical and structural properties may be related to the antibacterial activity of these nitrofuran-type compounds.

show abstract

“…titrimetric method [3,5], spectrophotometric methods [6,7], flow injection analysis [8], spectrofluorimetric method [9,10], electrochemical methods [11,12] and chromatographic methods [13][14][15][16]. No micellar liquid chromategraphic method for simultaneous determination of TIN is available in the literature Micellar liquid chromatography (MLC) is a form of reversed phase liquid chromatography that uses an aqueous micellar solution as the mobile phase.…”

Section: Introductionmentioning

confidence: 99%

Micellar high performance liquid chromatographic determination of tinidazole in combination with ciprofloxacin or norfloxacin in bulk, pharmaceutical dosage forms and in spiked human plasma

et al. 2014

View full text Add to dashboard Cite

A micellar liquid chromatographic method was developed for determination of tinidazole in bulk, dosage forms and human plasma using intersil cyano column and a mobile phase of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid:water (60:40, v:v) (pH = 4). The U.V. detection was achieved at 311 nm. Various chromatographic parameters were investigated to select the optimum conditions for the separation, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was linear over the concentration range 40-200 µg/mL with regression coefficient 0.999. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of tinidazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.Stability Tinidazole Method validation Anti-protozoal drugs Micellar liquid chromatography Optimization and system suitability

show abstract

Reduction of Different Nitro-Compounds on Electroenzymatic System: Cytochrome c Reductase-NAD(P)H Modified Carbon Paste Electrode

Cited by 6 publications

References 31 publications

Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode

Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode

Electronic and physicochemical properties of selected nitrofurans: A theoretical study

Micellar high performance liquid chromatographic determination of tinidazole in combination with ciprofloxacin or norfloxacin in bulk, pharmaceutical dosage forms and in spiked human plasma

Contact Info

Product

Resources

About