Reduction of Covalent Binding of Aflatoxin B1 to Rabbit Liver DNA After Immunization Against this Carcinogen

Schmidt

Environ Health Perspect

1993

The exposure to aflatoxin B, (AFB) in animal-feed processing plants was assessed using bindingofAFB to serum albumin. The albumin fraction was digested with pronase, and the digest was purified on a C18 Sepak column and an aflatest affinity colunm before quantification by ELISA. The level ofdetectability was5 pg/mg albumin. The workers served as their own controls, as blood samples were taken upon return from vacation and after 4 weeks ofwork. A total of 7 of45 samples were positive for AFB, with an estimated average daily intake of 64 ng AFB/kg body weight. The exposed workers had been disembarking cargos contaminated with AFB or working at places where the dust contained detectable amounts of AFB. The sera from the exposed workers had a significantly higher titer against an aflatoxin BI-epitope than a nonexposed Danish control group. The level ofexposurecould partly explain the increased risk of liver cancer in workers in the animalfeed processing industry.

Section: Resultsmentioning

confidence: 99%

Exposure to aflatoxin B1 in animal-feed production plant workers.

Schmidt

Environ Health Perspect

1993

“…Lower mortality and reduction of acute toxic effects in the liver were also demonstrated in immunized rabbits and rats challenged with a single dose of AFB 1 [ 120 , 121 , 122 , 123 ]. Following similar immunization experiments, a reduction of the covalent binding of AFB 1 to liver DNA was observed, indicating a possible reduced susceptibility for liver tumor formation [ 124 ]. Additional in vitro investigations proved that specific anti-AFB 1 antisera were able to decrease genotoxic and mutagenic effects of AFB 1 [ 125 , 126 ].…”

Section: Mitigation Of Afm 1 Occurrence In Milkmentioning

confidence: 99%

AFM1 in Milk: Physical, Biological, and Prophylactic Methods to Mitigate Contamination

Giovati

Magliani

Ciociola

et al. 2015

Toxins

115

Aflatoxins (AFs) are toxic, carcinogenic, immunosuppressive secondary metabolites produced by some Aspergillus species which colonize crops, including many dietary staple foods and feed components. AFB1 is the prevalent and most toxic among AFs. In the liver, it is biotransformed into AFM1, which is then excreted into the milk of lactating mammals, including dairy animals. AFM1 has been shown to be cause of both acute and chronic toxicoses. The presence of AFM1 in milk and dairy products represents a worldwide concern since even small amounts of this metabolite may be of importance as long-term exposure is concerned. Contamination of milk may be mitigated either directly, decreasing the AFM1 content in contaminated milk, or indirectly, decreasing AFB1 contamination in the feed of dairy animals. Current strategies for AFM1 mitigation include good agricultural practices in pre-harvest and post-harvest management of feed crops (including storage) and physical or chemical decontamination of feed and milk. However, no single strategy offers a complete solution to the issue.

Environmental Health Perspectives

“…Antibodies aginst a carcinogen epitope have been shown to decrease the uptake ofthe carcinogen after oral administration (11), inhibit carcinogen-DNA binding (12), and inhibit tumor? igenicity (13).…”

Section: Resultsmentioning

confidence: 99%

Exposure to Aflatoxin B 1 in Animal-Feed Production Plant Workers

Autrup¹,

Schmidt²,

Autrup³

1993

The exposure to aflatoxin B1 (AFB) in animal-feed processing plants was assessed using binding of AFB to serum albumin. The albumin fraction was digested with pronase, and the digest was purified on a C18 Sepak column and an aflatest affinity column before quantification by ELISA. The level of detectability was 5 pg/mg albumin. The workers served as their own controls, as blood samples were taken upon return from vacation and after 4 weeks of work. A total of 7 of 45 samples were positive for AFB, with an estimated average daily intake of 64 ng AFB/kg body weight. The exposed workers had been disembarking cargos contaminated with AFB or working at places where the dust contained detectable amounts of AFB. The sera from the exposed workers had a significantly higher titer against an aflatoxin B1-epitope than a nonexposed Danish control group. The level of exposure could partly explain the increased risk of liver cancer in workers in the animal-feed processing industry.