Reduction of chromium-vi by chromium resistant lactobacilli: A prospective bacterium for bioremediation

Mishra, Ritesh; Sinha, Vartika; Kannan, A.; Upreti, Raj K.

doi:10.4103/0971-6580.94512

Cited by 40 publications

(9 citation statements)

References 28 publications

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“…Some lactobacilli are Cr-resistant and would be useful for Cr detoxification and bioremediation, e.g. L. paracase CL1107 manifested in vitro Cr(VI) reduction ability, though not as competent as TW1-1 23 , 24 . TW1-1 reduced tissue absorption of Cr by promoting its excretion through feces, implying that TW1-1 might exert its effects in two possible ways.…”

Section: Discussionmentioning

confidence: 99%

Gut remediation: a potential approach to reducing chromium accumulation using Lactobacillus plantarum TW1-1

Xiao

Feng

et al. 2017

Sci Rep

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Some lactobacilli have protective effects against some heavy metals in mammals, but the underlying mechanism is not fully understood. To evaluate the remediation potency and the mechanism of Lactobacillus against chromium (Cr) in mice, Lactobacillus plantarum TW1-1 was orally administrated to Kunming mice for 7 weeks during exposure to 1 mM K2Cr2O7 in drinking water. Results showed that TW1-1 helped to decrease Cr accumulation in tissues and increase Cr excretion in feces, and may also attenuate alterations in oxidative stress and histopathological changes caused by Cr exposure. Moreover, the chromate reduction ability of fecal bacteria doubled after administration of TW1-1 upon Cr induction. MiSeq sequencing of fecal bacterial 16S rRNA genes revealed that the overall structures of gut microbiota was shifted by Cr exposure and partially restored by TW1-1. The abundances of 49 of the 79 operational taxonomic units altered by Cr were reversed by TW1-1. Based on these, we proposed a working model of TW1-1 against Cr: TW1-1 helps to remove Cr from the host and meanwhile acts as a regulator of gut microbiota, which aids in chromate reduction and provide protection against Cr. We call this process of remediation of heavy metal in the gut “gut remediation”.

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Section: Discussionmentioning

confidence: 99%

Gut remediation: a potential approach to reducing chromium accumulation using Lactobacillus plantarum TW1-1

Xiao

Feng

et al. 2017

Sci Rep

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“…Because of the diverse applications of chromium and its drastic impacts on humans and environment, there is an urgent need to design strategies to remove Cr(VI) from effluents. Recently, a number of bacterial strains have been isolated with the ability to reduce the highly toxic Cr(VI) to the less toxic Cr(III) (Mishra et al, 2012;Long et al, 2013;Voica et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Role of Microbial Mats in the Removal of Hexavalent Chromium and Associated Shifts in Their Bacterial Community Composition

et al. 2020

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Microbial mats are rarely reported for chromium-polluted ecosystems, hence information on their bacterial diversity and role in chromium removal are very scarce. We investigated the role of nine microbial mats, collected from three quarry sumps of chromium mining sites, in the removal of hexavalent chromium [Cr(VI)]. Bacterial diversity in these mats and community shifts after incubation with Cr(VI) have been investigated using MiSeq sequencing. In nature, a chromium content of 1,911 ± 100 mg kg −1 was measured in the microbial mats, constituting the third highest source of environmentally available chromium. The mats were able to remove 1 mg l −1 of Cr(VI) in 7 days under aerobic conditions. MiSeq sequencing of the original mats yielded 46-99% of the sequences affiliated to Proteobacteria, Firmicutes and Actinobacteria. When the mats were incubated with Cr(VI), the bacterial community shifted in the favor of Alphaproteobacteria and Verrucomicrobiae. We conclude that microbial mats in the quarry sumps harbor diverse microorganisms with the ability to remove toxic Cr(VI), hence these mats can be potentially used to remove chromium from polluted waters.

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“…These observations are in corroboration with Mishra et al . [ 33 ] wherein it is reported that the Cr-resistant probiotic LAB strains showed similar biochemical parameters as their respective normal strain.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, both were tested for antibiotic sensitivity by standard disc diffusion method[ 33 ] against Kanamycin (30µg), Norfloxacin (10 µg), gentamycin (10 µg), chloramphenicol (30 µg), amoxycillin (10 µg), ciprofloxacin (5 µg), and teicoplanin (30 µg). The antibiotic discs were procured from HiMedia.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of bioremediation capacity of a commercial probiotic, Bacillus coagulans, for chromium (VI) and lead (II) toxicity

2016

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Introduction:The bioaccumulation of heavy metals including chromium (VI) (Cr (VI)) and lead (II) (Pb (II)) causes fatal toxicity in humans. Some naturally occurring bacterial genera such as Bacillus and Pseudomonas help in bioremediation of these heavy metals and some of the species of Bacillus are proven probiotics. However, no study has been conducted on Bacillus coagulans, which is a proven probiotic species of genus Bacillus.Objectives:The primary objective of the present study was to assess the potential of a proven probiotic, B. coagulans, marketed as “Sporlac-DS,” to survive in the presence of Cr (VI) and Pb (II) and its ability to reduce its concentration in vitro.Materials and Methods:The Minimum inhibitory concentration (MIC) of the organism for Cr (VI) and Pb (II) was determined followed by its biochemical and morphological characterization. Its antibiotic sensitivity and probiotic efficacy were assessed. Further, its bioremediation capacity was observed in vitro by determining the residual Cr (VI) and Pb (II) concentration after 72 h.Results:B. coagulans could tolerate up to 512 ppm concentration of Cr (VI) and had an MIC of 128 ppm for Pb (II). After 72 h, the organism reduced 32 ppm Cr (VI) and 64 ppm Pb (II) by 93% and 89%, respectively. When B. coagulans was studied before and after growing on Cr (VI) and Pb (II) for 24 h, an increase was seen in sensitivity toward the tested antibiotics whereas no change was observed in morphological and biochemical characters. It also showed no change in their bile and acid tolerance, indicating that it retains its probiotic efficacy.Conclusion:The tested probiotic B. coagulans may have a potential role in bioremediation of Cr (VI) and Pb (II), in vivo.

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Reduction of chromium-vi by chromium resistant lactobacilli: A prospective bacterium for bioremediation

Cited by 40 publications

References 28 publications

Gut remediation: a potential approach to reducing chromium accumulation using Lactobacillus plantarum TW1-1

Gut remediation: a potential approach to reducing chromium accumulation using Lactobacillus plantarum TW1-1

The Role of Microbial Mats in the Removal of Hexavalent Chromium and Associated Shifts in Their Bacterial Community Composition

In vitro evaluation of bioremediation capacity of a commercial probiotic, Bacillus coagulans, for chromium (VI) and lead (II) toxicity

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