Reduction of chromatin heteroaggregates by acid precipitation of mammalian cell culture and ramification in protein A chromatography for recombinant immunoglobulin G purification

Combined clarification and affinity capture using magnetic resin enables efficient separation of rAAV5 from cell lysate

“…Firstly, acid precipitation is typically used to remove chromatin aggregates and other host cell contaminants. 33 This step is needed to avoid clogging of the clarification filters and takes, in our experience, around 8–12 h (often performed overnight). Second, the cell lysate is clarified by depth filtration and further purified, concentrated, and buffer exchanged by TFF.…”