Reduction of bacterial manifestation in the in vitro cultivation of Eucalyptus microcorys F. Muell

Atala, Laura Ribeiro; Faria, Júlio Cézar Tannure; Molinari, Letícia Vaz; Avelar, Maria Lopes Martins; Brondani, Gilvano Ebling

doi:10.1007/s42535-022-00360-z

Cited by 1 publication

(2 citation statements)

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“…It is noteworthy that, even with statistical differences in peroxidase activity, all propagation techniques carried out in this study resulted in a degree of tissue rejuvenation and/ or reinvigoration. Micropropagation has several advantages for the propagation of tree species, especially for ones that are difficult to root, whereas other techniques rarely succeed (Atala et al 2022;Faria et al 2022). Several studies recommend this propagation technique using epicormic shoots, mainly for species with high ontogenetic ages, for instance, for Eucalyptus benthamii Maiden & Cambage 13-years-old (Baccarin (Oliveira et al 2015).…”

Section: Peroxidase Activitymentioning

confidence: 99%

“…Among tree species, Eucalyptus microcorys F. Muell. has characteristics that are favourable to silvicultural applications, such as moderate resistance to frost, drought-resistant species, tolerance to fire, and good regeneration capacity by stump coppicing (Atala et al 2022). Despite its potential commercial applications, E. microcorys has not been established commercially nor it has been studied as often as other Eucalyptus species.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

et al. 2023

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Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro- and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the mini cuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.

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Section: Peroxidase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%