Reduction of aflatoxins by Rhizopus oryzae and Trichoderma reesei

Hackbart, Helen Cristina dos Santos; Machado, Alexandre Reis; Christ-Ribeiro, Anelise; Prietto, Luciana; Badiale‐Furlong, Eliana

doi:10.1007/s12550-014-0202-6

Cited by 44 publications

(26 citation statements)

References 20 publications

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“…The chromatographic parameters were a flow rate for the mobile phase 0.4 mL/min, and a column temperature of 45 C, resulting in a retention time of 12.5; 15.0; 18.5; and 22.4 min for AFLAG 2 , AFLAG 1 , AFLAB 2 and AFLAB 1 , respectively, at 25 min as a total running time. For the quantitation of the aflatoxins, the dry residue resulting from the extraction was dissolved with 0.2 mL of the mobile phase and manually injected on the HPLC-FL (Hackbart et al, 2014).…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Post-harvest operations and aflatoxin levels in rice (Oryza sativa)

et al. 2015

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Section: Chromatographic Conditionsmentioning

confidence: 99%

Post-harvest operations and aflatoxin levels in rice (Oryza sativa)

et al. 2015

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“…Although this parameter was not able to describe the differentiation between active and inactive fungal biomass; it tended to increase during fermentation periods and correlated with the total amount of fungal biomass increasing (Hackbart et al, 2014). According to Figure 2, the result of the glucosamine content was depended on the fermentation days.…”

Section: Resultsmentioning

confidence: 97%

“…The fungal biomass in the fermented corn was determined by estimating the presence of glucosamine in the cell wall of the culture, modified from Hackbart et al (2014). Before entering the main glucosamine determining procedures, samples were treated as the modifying of Smits et al (1996a).…”

Section: Glucosaminementioning

confidence: 99%

“…Sardjono et al (2004a) also supported that there were 5 protein fractions of extracellular enzyme produced by A. oryzae KKB4 in liquid synthetic medium. Moreover, the references have explained varied production and activity of AFB1 degrading enzymes, depending on type of substrate, moisture content, fermentation system, and environmental controlling (Guan et al, 2008;Hackbart et al, 2014). The AFB1 reduction is rarely correlated with the fungal biomass.…”

Section: Reduction Of Afb1 By Aspergillus Oryzae Kkb4mentioning

confidence: 99%

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The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Carolina¹,

Setyabudi²,

Sardjono³

2017

MJM

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Aims:The growth and metabolic activity of Aspergillus oryzae KKB4 in AFB1-contaminated corn and later coincided with AFB1 reduction and detoxification were investigated. Methodology and results:The decreasing of AFB1 amount by A. oryzae KKB4 could be clearly observed if the initial AFB1 concentration in corn was increased. Thus, moist-heated corn was artificially inoculated with Aspergillus flavus FNCC 62C7 to increase AFB1 content. AFB1-contaminated corn was applied as solid substrate and then inoculated with A. oryzae KKB4. During fermentation periods, the growth, metabolic activity, and AFB1 decline were investigated by glucosamine content, water content loss, and AFB1 concentration, respectively. The maximum growth was occurred in 4 th day at 1.499 ± 0.028 g glucosamine/ 100 g dry matter. The metabolic activity was going on up to the end of fermentation days, as shown as water content loss at 0.175 ± 0.007 g/g dry matter. In accordance with the growth and metabolic activity, the amount of AFB1 reduction was 37.04 ng AFB1/g dry matter during 5 days fermentation. According to toxicity analysis, it was found that the residues of AFB1 were not toxic to Bacillus megaterium cells. Conclusion, significance and impact of study: A. oryzae KKB4 is able to be applied in solid substrates as AFB1 reduction and detoxification agent. These lucrative effects are also important in relation with food and feed safety.

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“…Non-toxigenic fungus competes with close related strain to meet nitrogen, carbon, water and mineral (Ehrlich 2014). Rhizopus oligosporus (BCC F0216), Saccharomyces cerevisiae (BCC F0206) (Kusumaningtyas et al 2006), Rhizopus oryzae (CCT 7560) and Trichoderma reesei (QM9414) (Hackbart et al 2014) were also reported as biological control for Aspergillus flavus or aflatoxin.…”

Section: Introductionmentioning

confidence: 99%

Rhizopus oligosporus Activity in Crude Extract and Powder Form to Reduce Aspergillus flavus and Aflatoxin Contamination in Corn

Kusumaningtyas¹,

Masrianti

Fitrya

2019

JITV

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Rhizopus oligosporus (RO) in isolate culture was known to reduce contamination toxigenic mold Aspergillus flavus (AF) and aflatoxin B1 in chicken feed. Application in culture form was not effective. The aim of this research was to evaluate RO activities in extract and inoculum form to reduce contamination of AF and aflatoxin B1 in corn. RO was harvested from agar plate, blended, added with water (ratio 1:1 (w/v)) and centrifuged. Supernatant was filtered using Whatman 41. Inoculum was made by inoculation RO in soy powder and incubated at 28oC for 5 days. Inoculum was dried at 40oC for 24-28 hours. Assay was conducted by addition extract or inoculum to corn. Extract and corn ratio were 1:1 (v/w), while inoculum doses were 5, 25, 50, 1000 dan 200 g/kg corn. Assay for aflatoxin B1 was done using kit ELISA aflatoxin. The result of this research showed that extract was able to reduce AF contamination up to 1 log 10, while the less concentration of inoculum which able to inhibit AF up to 6 log 10 was 100 g/kg corn. Extract RO 125 and 250 mL/kg corn was able to reduce aflatoxin contamination by 93.69 % and 85.84 %. Inoculum at dose 5 and 100 g/kg corn was able to reduce aflatoxin 57.58% and 85%. Based on the result, it could be concluded that RO in extract or inoculum form was able to reduce contamination of AF and aflatoxin B1 in corn. Rhizopus as inoculum was easier to be applied than in extract form.

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Reduction of aflatoxins by Rhizopus oryzae and Trichoderma reesei

Cited by 44 publications

References 20 publications

Post-harvest operations and aflatoxin levels in rice (Oryza sativa)

Post-harvest operations and aflatoxin levels in rice (Oryza sativa)

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Rhizopus oligosporus Activity in Crude Extract and Powder Form to Reduce Aspergillus flavus and Aflatoxin Contamination in Corn

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