Reduction of aflatoxin B1 in chicken feed by using Saccharomyces cerevisiae, Rhizopus oligosporus and their combination

Abstract

Aflatoxin B1 is a toxigenic and carcinogenic compound produced by Aspergillus flavus and Aspergillus parasiticus. An approach to prevent aflatoxin contamination in feed was carried out by using Saccharomyces cerevisiae (Sc) and Rhizopus oligosporus (Ro). Aspergillus flavus was cultured together with Sc, Ro and their combination (ScRo) in chicken feed. The aflatoxin B1 content was observed at day 0, 5, 10 and 15. The result showed that aflatoxin B1 contaminations in feed were reduced by Sc, Ro and ScRo addition. The highest reduction of aflatoxin B1 content was shown at day 5 for all treatments with Sc, Ro and ScRo. The best activity of reducing aflatoxin B1 was shown by Ro. Although the ability of reducing aflatoxin B1 of Sc, Ro or ScRo was not significantly different, Sc or Ro gave the better result than ScRo and they are better used individually.

In vivo antifungal activity of neem oil and aqueous extracts against leaf spot disease caused by <i>Cercospora abelmoschii</i> on okra

“…All these genera have been reported for other microorganisms controlling on biocontrol tests (Bettiol et al, 2012;kusumaningtyas et al, 2006;Ziedan et al, 2013). Moreover, there are many products already market based on fungi for the biological control of pathogens in plants, whose effectiveness is proven (Bettiol et al, 2012).…”

Several Functions of Rhizopus sp on Increasing Nutritional Value of Feed Ingredient

“…Sebagai pengendali A. flavus dan aflatoksin, Rhizopus sp bekerja dengan cara menghambat pertumbuhan kapang A. flavus dan/atau menurunkan konsentrasi aflatoksin yang sudah terbentuk. Rhizopus sp juga dapat menghambat pertumbuhan kapang A. flavus melalui mekanisme kompetisi dan kemampuannya untuk menghasilkan metabolit yang menghambat pertumbuhan kompetitor (Kusumaningtyas et al 2006). Kapang R. arrhizus memproduksi metabolit seperti etanol, isobutilalkohol dan 3-metil butanol yang dapat menghambat pertumbuhan A. flavus (Lanciotti & Guerzoni 1993).…”

“…Aplikasi Rhizopus sebanyak 1,5 g/kg pakan mampu menurunkan residu aflatoksin B1 pada hati bebek pada tingkat yang tidak terdeteksi dibandingkan dengan itik tanpa perlakuan dengan residu aflatoksin B1 0,0184 ppb. Residu aflatoksin M1 pada hati bebek juga lebih rendah pada bebek yang diberi imbuhan pakan Rhizopus yaitu 0,0021 ppb dari 0,0035 ppb pada bebek dengan pakan tanpa imbuhan inokulum Rhizopus (Kusumaningtyas et al 2006). Selain mendegradasi racun aflatoksin, Rhizopus sp dapat memecah alkaloid atau racun yang terkandung dalam biji jarak pagar, yaitu lectin/curcin, phorbolester/diterpene esters, tanin, fitat, saponin dan anti-trypsin (Wina et al 2012).…”

Detoxification of Groundnut Cake Naturally Contaminated with Aflatoxin B1 Using Rhodococcus erythropolis in Shake Flask Bioreactors

“…Other reported bacterial strains are Rhodococcus erythropolis [15], Pseudomonas aeruginosa [16], P. putida [17] and Myxococcus fulvus [18]. Some microorganisms act as toxin adsorbents, which include members of lactic acid bacteria such as Streptococcus lactis, Lactobacillus rhamnosus [19,20] and the yeast Saccharomyces cerevisiae [21].…”