Reduction of adhesion formation with cross-linked hyaluronic acid after peritoneal surgery in rats

Koçak, İ̇dris; Ünlü, Cihat; Akçan, Yusuf; Yakın, Kayhan

doi:10.1016/s0015-0282(99)00368-4

Cited by 40 publications

(21 citation statements)

References 17 publications

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“…Rodent models closely approximate the clinical presentation of this Th1-mediated inflammatory tissue response (10,(12)(13)(14), and adhesion formation involves the cecum, large bowel, and abdominal wall (Fig. 1A).…”

Section: Prevention Of Postsurgical Fibrosis By Zps Treatmentmentioning

confidence: 90%

Modulation of surgical fibrosis by microbial zwitterionic polysaccharides

Ruiz-Perez

Chung

Sharpe

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Section: Prevention Of Postsurgical Fibrosis By Zps Treatmentmentioning

confidence: 90%

Modulation of surgical fibrosis by microbial zwitterionic polysaccharides

Ruiz-Perez

Chung

Sharpe

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…It was demonstrated in CAPD patients that a single dialysate exchange with fluid supplemented with hyaluronan in concentrations of 0.1 or 0.5 g/l does not cause any adverse effects [8]. Presence of exogenous high-molecular-weight hyaluronan in the peritoneal cavity may not only reduce intraperitoneal inflammation but also reduce formation of peritoneal adhesions [9, 10]and this effect may be due to an increase in the fibrinolytic activity of mesothelial cells exposed to inflammatory cytokines [11]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Haluronan-Supplemented Dialysate on in vitro Function of Human Peritoneal Mesothelial Cells

et al. 2004

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Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p < 0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

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“…Vascular proliferation score is graded on a 0-3 scale: 0: no vascular proliferation, 1: mild vascular proliferation, 2: moderate vascular proliferation, and 3: dense vascular proliferation. 13,14 …”

Section: Macroscopic and Microscopic Measurementsmentioning

confidence: 99%

A Novel Method of Comparing Anti-Adhesive Agents Used to Prevent Postoperative Peritoneal Adhesions

Hatipoğlu¹,

Demiryas²,

Arıkan³

et al. 2018

TJCD

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Amaç: Bu çalışmada amacımız yeni bir objektif adezyon değerlendirme yönteminden yararlanmak ve sodyum hiyalüronat ve karboksimetilselüloz membran (HA-CMC), polietilen glikol lisin (PEG-L) ve sodyum hiyalüronik asit (HA) gibi sıklıkla kullanılan anti-adeziv ajanların etkinliğini karşılaştırmaktır. Yöntem: Çekum abrazyonu, bir kısmında post-op 5. günde (grup 1) ve diğerlerinde post-op 14. günde (grup 2) oluşturulmak üzere iki eşdeğer gruba randomize edilen 64 dişi Wistar albino sıçanında (250-300 g) uygulandı. Her bir grup, uygulanan anti-adeziv ajana göre; PEG-L, HA-CMC, HA ve kontrol grubu olmak üzere dört alt gruba ayrıldı. Adezyonlar, geleneksel yöntemlerle ve yeni bir yöntem olan "Bölge Hesaplama" yöntemi ile değerlendirildi. Bulgular: Birinci grupta herhangi bir değerlendirme aracı ile anlamlı fark bulunamadı. Bununla birlikte, ikinci grubun PEG-L alt grubunda, bölge hesaplama yöntemi ile yapışma bölgesi skoru daha yüksekti. Aynı alt grubun histopatolojik değerlendirmesinde daha yüksek enflamasyon skoru bulundu; ancak, diğer sonuçlar benzerdi. Sonuç: Histopatolojik sonuçlar ve makroskopik değerlendirmeler tüm gruplarda benzer olmakla birlikte, bölge hesaplama yöntemi ile PEG-L alt grubunda adezyon artışı gösterildi. Yapışma konvansiyonel değerlendirme yöntemleri yarı kantitatif olarak kabul edilirken, bölge hesaplama yöntemi subjektif değerlendirmelere izin vermemektedir. Bu yöntemin geleneksel yöntemlerin yerini alabileceğine ve anti-adeziv ajanları/materyalleri değerlendiren çalışmalarda kullanılabileceğine inanıyoruz. Anahtar Kelimeler: Sepracoat, seprafilm, SprayShield, yapışıklık önlenmesi, hayvan deneyi Aim: In this study, our aim was to utilize a novel and objective method of adhesion evaluation and compare the effectiveness of anti-adhesive properties of commonly used agents, such as sodium hyaluronate and carboxymethylcellulose membrane (HA-CMC), polyethylene glycol lysine (PEG-L), and sodium hyaluronic acid (HA). Method: Cecal abrasion was performed on 64 female Wistar albino rats (250-300 g) which were randomized into two equivalent groups, one group was sacrificed on postoperative day 5 (group 1), and the other on postoperative day 14 (group 2). Each group was subcategorized to four groups according to applied anti-adhesive material: PEG-L, sodium HA-CMC, HA, and control group. Adhesions were evaluated with conventional methods and the novel "Zone Calculation" method. Results: In group 1, no significant differences were found with any of the evaluation tools. However, in the PEG-L subgroup of the group 2, adhesion zone score was higher with the zone calculation method. The same subgroup was found to have a higher inflammation score on histopathological evaluation; however, other results were similar. Conclusion: Although histopathological results and macroscopic evaluations were similar in all groups, the zone calculation method indicated greater adhesion in the PEG-L subgroup. Conventional evaluation methods of adhesion are considered to be semi-quantitative, whereas the zone calculation...

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Reduction of adhesion formation with cross-linked hyaluronic acid after peritoneal surgery in rats

Cited by 40 publications

References 17 publications

Modulation of surgical fibrosis by microbial zwitterionic polysaccharides

Modulation of surgical fibrosis by microbial zwitterionic polysaccharides

Effect of Haluronan-Supplemented Dialysate on in vitro Function of Human Peritoneal Mesothelial Cells

A Novel Method of Comparing Anti-Adhesive Agents Used to Prevent Postoperative Peritoneal Adhesions

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