Reduction of actin-related protein complex 2/3 in fetal Down syndrome brain

Weitzdoerfer, Rachel; Fountoulakis, Michael; Lubec, Gert

doi:10.1016/s0006-291x(02)00291-7

Cited by 91 publications

(50 citation statements)

References 34 publications

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“…A similar approach was also used to evaluate expression levels of actin-binding proteins in fetal Down's syndrome brain and control cortex (53). The authors (53) noted a significant reduction of the actin-related protein complex 2/3 (Arp2/3) 20-kDa subunit and the coronin-like protein p57, which are involved in actin filament cross-linking and nucleation and capping of actin filaments.…”

Section: Proteomics Analysis Of Nervous Systemmentioning

confidence: 99%

Proteomic Analysis in the Neurosciences

Morrison

Kinoshita

Johnson

et al. 2002

Molecular & Cellular Proteomics

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Section: Proteomics Analysis Of Nervous Systemmentioning

confidence: 99%

Proteomic Analysis in the Neurosciences

Morrison

Kinoshita

Johnson

et al. 2002

Molecular & Cellular Proteomics

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“…Expression of both of these transcripts has been examined in conjunction with other diseases. ARPC2, also called Arp2/3 plays a critical role in actin polymerization and has been implicated in Down's syndrome (Weitzdoerfer et al, 2002) and may be involved in dendritic spine abnormalities associated with schizophrenia (Irie and Yamaguchi, 2004). Less is known about the function and regional localization of MOBP.…”

Section: Discussionmentioning

confidence: 99%

Molecular mapping of striatal subdivisions in juvenile Macaca Mulata

O’Connor

Muly

Hemby

2006

Experimental Neurology

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The striatum of the primate brain can be subdivided into three distinct anatomical subregions: caudate (CAU), putamen (PUT), and ventral striatum (VS). Although these subregions share several anatomical connections, cell morphological, and histochemical features, they differ considerably in their vulnerability to different neurological and psychiatric diseases, and these brain regions have significantly different functions in health and disease. In order to better understand the molecular underpinnings of the different disease and functional vulnerabilities, transcriptional profiles were generated from the CAU, PUT, and VS of five juvenile rhesus macaques (Macaca mulatta) using human cDNA neuromicroarrays containing triplicate spots of 1227 cDNAs. Differences in microarray gene expression were assessed using z score analysis and 1.5-fold change between paired subregions. Clustering of genes based on dissimilarity of expression patterns between regions revealed subregion specific expression profiles encoding Gprotein-coupled receptor signaling transcripts, transcription factors, kinases and phosphatases, and cell signaling and signal transduction transcripts. Twelve transcripts were examined using quantitative real-time PCR (qPCR), and 81% demonstrated alterations similar to those seen with microarray analysis, some of which were statistically significant. Subregion specific transcription profiles support the anatomical differentiation and potential disease vulnerabilities of the respective subregions.

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“…Reduced expression was also observed for the structural constituent of the cytoskeleton neurofilament L [158] and for stathmin [144]. In the fetal DS brain, reduced levels were found for actinrelated protein complex 2/3 20 kDa and coronin-like protein p57 [159]. These actin-binding proteins are involved in actin filament cross-linking and nucleation and capping of actin filaments and their deficient levels may, at least partially, be related with the brain dysgenesis in DS.…”

Section: Structural Proteinsmentioning

confidence: 97%

Proteomics‐driven progress in neurodegeneration research

Fountoulakis

Κοσσίδα

2006

Electrophoresis

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Proteomics-driven progress in neurodegeneration researchProteomics technologies have been widely used in the investigation of neurodegenerative and psychiatric disorders, and in particular in the detection of differences between healthy individuals and patients suffering from such diseases. Thus, brain and cerebrospinal fluid (CSF) samples from patients with Alzheimer's disease, Down syndrome, Pick's disease, Parkinson's disease, schizophrenia, and other disorders as well as brain and CSF from animals serving as models of neurological disorders have been analyzed by proteomics. 2-DE followed by MALDI-TOF-MS has been mainly applied as this proteomics approach provides the possibility of convenient quantification of protein levels and detection of post-translational modifications. About 330 unique proteins with deranged levels and modifications have been detected by proteomics approaches to be related to neurodegeneration and psychiatric disorders. They are mainly involved in metabolism pathways, cytoskeleton formation, signal transduction, guidance, detoxification, transport, and conformational changes. In this article, we provide a summary of the major contributions of proteomics technologies in the study of neurodegenerative and psychiatric diseases, in particular, in the detection of changes in protein levels and modifications related to these disorders. IntroductionNeurodegeneration is a major hallmark of a series of genetic and acquired disorders of the central nervous system (CNS), like Alzheimer's disease (AD), Down syndrome (DS), Pick's disease, Creutzfeldt-Jakob disease (CJD), and Parkinson's disease, which result in severe cognitive and motor deficits. A common characteristic of these diseases is that they are multifactorial.Their molecular basis is unknown and individual biomarkers are not reliable. Genomics and proteomics approaches have been applied to obtain a better understanding of neurodegeneration and psychiatric disorders. Genomics has been used to map and identify genes that are mutated in a wide variety of such disorders, like presenilins in AD. Next to genomics, which only provides a partial view of the biological problem, proteomics is widely used in clinical diagnosis as well and many changes in protein levels associated with specific diseases have been identified [1,2].Proteomics is a technology-driven science that studies the proteins of the various biological systems, their structures, interactions, post-translational modifications, and in particular the changes in their levels and modifications, which are the result of specific diseases or are induced by external factors.

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Reduction of actin-related protein complex 2/3 in fetal Down syndrome brain

Cited by 91 publications

References 34 publications

Proteomic Analysis in the Neurosciences

Proteomic Analysis in the Neurosciences

Molecular mapping of striatal subdivisions in juvenile Macaca Mulata

Proteomics‐driven progress in neurodegeneration research

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