Reduction in the Number of Infective Trichinella spiralis Larvae in Mice by Use of Homeopathic Drugs

Abstract

Background: Trichinellosis caused by the gastrointestinal nematode Trichinella spiralis occurs in humans, domestic animals and wild animals. It is difficult to control the muscle phase of the parasite. Homeopathic drugs such as Cina and Santoninum have anthelmintic properties. We have observed that in material doses, the homeopathic drug Podophyllum also has nematotoxic properties. We have also observed that homeopathic potency can influence the water permeability of cells. Objective: The purpose of this study was to investigate whether potentized homeopathic drugs such as Cina 30, Santoninum 30 and Podophyllum mother tincture can affect the muscle phase of the parasite T. spiralis in mice. Another objective was to see whether trichinellosis and its treatment with the 3 named homeopathic drugs could alter the water content in the muscle tissue of mice. Materials and Methods: Cina 30 and Santoninum 30 were prepared from the mother tincture of the flowering tops of Artemisia nilagirica and its active principle santonin, in each case by successive dilution (1:100) with 90% ethanol and sonication in 30 steps following the single glass method (K30). Ethanol 30 was prepared by successive dilution of 90% ethanol with 90% ethanol (1:100) followed by sonication in 30 steps. In each step, the dilution was sonicated at 20 KHz for 30 s. We have observed before that sonication is a more uniform, measurable and effective process of mechanical agitation of a liquid than manual succussion. Experimentally infected mice were orally treated with an aqueous Podophyllum suspension at 60 mg/kg/day. Each potentized drug was diluted 1:20 with distilled water and administered orally at 0.05 ml/mouse/day. Each mouse was inoculated with T. spiralis larvae at a dose of 200 larvae/mouse by esophageal intubation. Treatment was started on day 7 post-infection and continued for 120 days. After completion of treatment, the mice were sacrificed and the larvae were extracted from muscles by HCl-pepsin digestion. The water content of the muscles was measured by determining the difference between fresh weight and dry weight of the tissue. Results: Podophyllum ?, Cina 30 and Santoninum 30 reduced the larval population in the studied mice by 68.14%, 84.10% and 81.20%, respectively, as compared to the untreated control group. Ethanol 30 achieved no significant reduction in the larval population compared to the untreated control group. The water content of the muscle tissue in the untreated control group and the Podophyllum-treated groups was significantly higher than in the Ethanol 30-, Cina 30- and Santoninum 30-treated groups. Conclusions: (1) Podophyllum ?, Cina 30 and Santoninum 30 were effective in the muscle phase of T. spiralis infection and significantly reduced the larval population in the treated mice. The potencies were more effective than the mother tincture, an effect which was not due to the medium ethanol. (2) The potencies significantly reduced the water content of the muscle tissue which might have affected the larvae. The effect of Po...

Efficacy of Lasia spinosa leaf extract in treating mice infected with Trichinella spiralis

“…However, the detailed mechanisms of these influences remain unclear. In another related study, Sukul et al (2005) studied the effects of potentized homeopathic drugs, Cina 30 (obtained from Artemisia nilagirica), Santoninum 30 and Podophyllum mother tincture (prepared from Podophyllum hexandrum) on muscle phase of the parasite T. spiralis in mice and observed that potentized drugs such as Cina 30 and Santoninum 30 were more effective (84.10% and 81.20%, respectively) in reducing the larval population in the muscles of mice than the crude extract of Podophyllum (68.14%). It thus appears from these studies that different plant extracts and/or their products vary in their effectiveness to different stages of T. spiralis but by and large, their efficacy appears to be comparatively low against the encysted larvae of parasite.…”

Animal models for studying homeopathy and high dilutions: Conceptual critical review

“…Cina 30c and Santoninum 30c were effective in reduction of Trichinella spiralis larvae infection in muscle tissue. 60 Lira-Salazar and co-workers, demonstrated the effect of Eupatorium perfoliatum 30cH and Arsenicum album 30cH on Plasmodium berghei infected mice. 13 In both cases parasite multiplication was inhibited, but the most interesting observation was the increase in the number of schizonts (the Plasmodium parasite form found inside the red blood cells), suggesting an interruption of Plasmodium life cycle in this intracellular phase.…”

Viremedy Effects in Severe Contaminations with some Infectious Organisms in Laboratory Mice

“…[ 17 , 18 , 19 , 20 ]] Due to the limitations in the present study and the fact that the number of tachyzoites should have been the same in all intraperitioeal injections to the laboratory mice, the number of tachyzoites injected to each mouse was 2×10 3 , which has been greatly higher than the "Lowest" Lethal Dose; LD100. ]] Due to the limitations in the present study, and considering the importance of the expedient equality of the number of the injected bacteria to the mice, the amount of the intraperitioeally-injected bacteria was 2×10 5 , which was much less than the "Lowest" Lethal Dose; LD 100 .…”