Reduction in hyperhydricity and improvement in in vitro propagation of commercial hard fibre and medicinal glycoside yielding Agave sisalana Perr. ex Engelm by NaCl and polyethylene glycol

Nikam, Tukaram D.; Mulye, Ketki V.; Chambhare, Mahadev R.; Nikule, Harichandra A.; Ahire, Mahendra L.

doi:10.1007/s11240-019-01603-9

Cited by 12 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In another study, PEG (0.1%) in combination with NaCl (0.2%) was used as osmotic stress-inducing agents to solve the problem of hyperhydricity during in vitro propagation of Agave sisalana. Plants subjected to the treatment of these additives have demonstrated the minimum hyperhydricity symptoms [ 17 ]. The use of silica gel to absorb excess water in a cultural medium was ineffective and promoted the development of abnormal shoots of Agastache foeniculum [ 16 ].…”

Section: Influence Of Medium Composition and Culture Conditions On Hy...mentioning

confidence: 99%

Hyperhydricity in Plant Tissue Culture

Polivanova

Bedarev

2022

Plants

View full text Add to dashboard Cite

Hyperhydricity is the most common physiological disorder in in vitro plant cultivation. It is characterized by certain anatomical, morphological, physiological, and metabolic disturbances. Hyperhydricity significantly complicates the use of cell and tissue culture in research, reduces the efficiency of clonal micropropagation and the quality of seedlings, prevents the adaptation of plants in vivo, and can lead to significant losses of plant material. This review considers the main symptoms and causes of hyperhydricity, such as oxidative stress, impaired nitrogen metabolism, and the imbalance of endogenous hormones. The main factors influencing the level of hyperhydricity of plants in vitro are the mineral and hormonal composition of a medium and cultivation conditions, in particular the aeration of cultivation vessels. Based on these factors, various approaches are proposed to eliminate hyperhydricity, such as varying the mineral and hormonal composition of the medium, the use of exogenous additives, aeration systems, and specific lighting. However, not all methods used are universal in eliminating the symptoms of hyperhydricity. Therefore, the study of hyperhydricity requires a comprehensive approach, and measures aimed at its elimination should be complex and species-specific.

show abstract

Section: Influence Of Medium Composition and Culture Conditions On Hy...mentioning

confidence: 99%

Hyperhydricity in Plant Tissue Culture

Polivanova

Bedarev

2022

Plants

View full text Add to dashboard Cite

show abstract

“…For eld emission scanning electron microscopy analysis, leaf samples of colchicine induced tetraploid and diploid plants were cut into 1.0 cm 2 pieces and xed in 1.25% glutaraldehyde (Sigma Aldrich, USA) in phosphate buffer saline (PBS) at room temperature for 12 h. The xed samples were washed with PBS and followed by dehydration in increasing graded series (10,25,50,75, and 100%) of ethyl alcohol 30 min into each grade (Nikam et al 2019). The leaf sample pieces were further dried and mounted on aluminum stub with auto-stick carbon tape and coated with gold in an auto ne Sputter Coater machine (Quorum Q150T ES).…”

Section: Field Emission-scanning Electron Microscopy (Fe-sem) Analysi...mentioning

confidence: 99%

In vitro induction and assessment of tetraploid plants from shoot cultures of diploid Niger (Guizotia abyssinica (L.f.) Cass.): a multipurpose oilseed crop

Chambhare

Nikam

2021

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

Agronomic traits improvement in crop plant can be accomplished by induction of polyploidy. Niger (Guizotia abyssinica (L.f.) Cass.) is one of the important edible oil yielding diploid crop (2n = 30). In the present study, the tetraploidization in Niger plants was achieved by treating apical portion of in vitro raised shoots with colchicine and their con rmation by chromosome counting and ow cytometry. The in vitro shoots were raised from leaf explants on MS medium supplemented with 1.0 mg/l BAP (6benzylaminopurine). The survival and nature of growth of treated shoots was variable with colchicine concentration (0.0, 0.005, 0.01, 0.02, 0.03, and 0.04%) and exposure time (4, 8, 12, and 16 h). The maximum tetraploid induction ratio was recorded with 0.02% colchicine treatment for 8 h which yielded 38.4% tetraploids. The chromosome number in root cells of tetraploid plantlets was 2n=4x=60 and the DNA content in leaf cells was 10.34 pg which was double to the diploid plant (4.70 pg) cells. In addition, there was signi cant difference exist in leaf characteristics of diploid and tetraploid plantlets. Compared to diploid plantlets, the tetraploid plantlets showed larger leaves, larger stomatal size, low stomatal index, larger capitula, larger seeds, and a greater number of seeds per capitula. The oil content in seeds was higher and associated with altered fatty acid pro le. The results demonstrated that the tetraploid plantlets obtained in this study exhibited some superior agronomical traits (as mentioned above) compared to diploid. The developed protocol and produced tetraploid plantlets will open the new door in improvement of edible oil yielding crop Guizotia abyssinica (L.f.) Cass.

show abstract

“…The majority of the previous literature focused on the prevention the occurrence of hyperhydricity in advance, while a few studies reported the reversion of hyperhydric shoots to their normal growth in order to improve the performance of in vitro propagation of some species, including the banana plant [ 19 ]; Agave sisalana Perr. [ 20 ]; and Dianthus chinensis L. [ 17 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Medium Composition and Environmental Culture Condition Enhances Antioxidant Enzymes, Recovers Gypsophila paniculata L. Hyperhydric Shoots and Improves Rooting In Vitro

Mohamed

El-Mahrouk

El-Banna

et al. 2023

Plants

View full text Add to dashboard Cite

Gypsophila paniculata L. is one of the most important commercial cut flowers worldwide. The plant is sterile and propagated mainly by in vitro culture techniques. However, hyperhydricity hinders its micropropagation and increases mortality during ex vitro acclimatization. Hyperhydric shoots of G. paniculata were proliferated from nodal explants on MS medium without growth regulators that contained 30 g L−1 sucrose, and gelled with 6.0 g L−1 agar. Medium components and environmental culture conditions were optimized to revert hyperhydricity in G. paniculata microshoots and develop an efficient micropropagation protocol for commercial production. Multiple shoots with high quality were successfully regenerated on MS medium fortified with potassium and ammonium nitrate at full concentration, 2.0 mg L−1 paclobutrazol, solidified with 9.0 g L−1agar in Magenta boxes of 62.87 gas exchange/day and incubated under light density of 60 µmol m−2s−1. We recorded 4.33 shoots, 40.00 leaves, 6.33 cm, 2.50 g and 95.00% for number of shoots/explant, number of leaves/shoot, shoot length, shoot fresh weight and normal shoots percentage, respectively. Well-rooted plantlets of G. paniculata were developed from the reverted microshoots, with the rooting percentage (95.00%) on MS medium augmented with 1.0 mg L−1 IBA in Magenta boxes of 62.87 gas exchange/day and 60 µmol m−2s−1 light density. In vitro-rooted plantlets exhibited reduced electrolyte leakage, and enhanced antioxidant enzymes activity of peroxidase, catalase, and polyphenol oxidase due to good ventilation at the highest gas exchange rate of the culture vessels.

show abstract

Reduction in hyperhydricity and improvement in in vitro propagation of commercial hard fibre and medicinal glycoside yielding Agave sisalana Perr. ex Engelm by NaCl and polyethylene glycol

Cited by 12 publications

References 34 publications

Hyperhydricity in Plant Tissue Culture

Hyperhydricity in Plant Tissue Culture

In vitro induction and assessment of tetraploid plants from shoot cultures of diploid Niger (Guizotia abyssinica (L.f.) Cass.): a multipurpose oilseed crop

Optimizing Medium Composition and Environmental Culture Condition Enhances Antioxidant Enzymes, Recovers Gypsophila paniculata L. Hyperhydric Shoots and Improves Rooting In Vitro

Contact Info

Product

Resources

About