Reducing ZnO nanoparticle cytotoxicity by surface modification

Luo, Mingdeng; Shen, Cenchao; Feltis, Bryce; Martin, Lisandra L.; Hughés, A.E.; Wright, Paul; Turney, Terence W.

doi:10.1039/c4nr00458b

Cited by 101 publications

(76 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the presence of LNA, ZnO NPs and BSA-induced Zn ion accumulation was not significantly affected in HepG2 cells. Previous work showed that the presence of vitamin C enhanced the toxicity of ZnO NPs due to the enhanced accumulation of intracellular Zn ions [6], whereas surface modifications of ZnO NPs could reduce the cytotoxicity of NPs by the reduction of NP uptake and, subsequently, the accumulation of intracellular Zn ions [37]. Thus, the unaltered cytotoxicity of ZnO NPs and BSA to HepG2 cells with the presence of LNA could be explained in that LNA did not significantly promote the accumulation of Zn ions into the cells.…”

Section: Discussionmentioning

confidence: 99%

The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

et al. 2017

View full text Add to dashboard Cite

Background: Nanoparticles (NPs) entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA) and NPs. Methods: This study used α-linolenic acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results: The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis, fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells, associated with an increase of intracellular Zn ions, but not intracellular superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-6) was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA was present. The presence of LNA did not significantly affect all of these endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from the present study indicated that BSA-complexed LNA might modestly interact with ZnO NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in HepG2 cells.

show abstract

Section: Discussionmentioning

confidence: 99%

The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…TiO 2 shell coating of ZnO NPs was reported to decrease their toxicity by controlling the rate of Zn 2+ ion release when interacting with cells [34]. Alternatively, alteration of surface properties of ZnO NPs, which may in turn affect their uptake rather than changing dissolution characteristics of the material, was recommended by Luo et al [35]. Novel strategies specifically targeting the dissolution tendency of ZnO NPs may help to enhance safe use of these materials in the biomedical area.…”

Section: Zno Nps Cell Interaction and Cytotoxicitymentioning

confidence: 97%

Biological Reactivity of Zinc Oxide Nanoparticles With Mammalian Test Systems: an Overview

Saptarshi

Duschl

Lopata

2015

Nanomedicine (Lond.)

102

View full text Add to dashboard Cite

Zinc oxide nanoparticles (ZnO NPs) have useful physicochemical advantages, and are used extensively. This has raised concerns regarding their potential toxicity. ZnO NP attributes that contribute to cytotoxicity and immune reactivity, however, seem to vary across literature considerably. Largely, dissolution and generation of reactive oxygen species appear to be the most commonly reported paradigms. Moreover, ZnO NP size and shape may also contribute toward their overall nano-bio interactions. Analysis is further complicated by factors such as adsorption of proteins on the NP surface, which may influence their bioreactivity. The main aim of this review is to give a systematic overview of the postulates explaining cytotoxic, inflammatory and genotoxic effects of ZnO NPs when exposed to different types of cells in vitro and in vivo.

show abstract

“…There is a degree of commonality across studies, in which smaller NPs tend to exhibit greater cytotoxicity (on a per mass basis) and that the required concentrations for cytotoxicity tend to be very high, while ZnO NPs are as well tolerated as zinc ions (Xia et al 2008;Feltis et al 2012;Shen et al 2013Shen et al , 2014. The mechanism for cytotoxicity relates to the elevated production of intracellular ROS via zinc displacement of other transition metal ions that are redox active, and is a function of particle surface area and coating, cellular uptake and lysosomal dissolution (Fukui et al 2012;Sharma et al 2012;James et al 2013;Shen et al 2013;Luo et al 2014).…”

Section: Introductionmentioning

confidence: 97%

ZnO nanoparticles and organic chemical UV-filters are equally well tolerated by human immune cells

et al. 2016

Self Cite

View full text Add to dashboard Cite

An important part of assessing the toxic potential of nanoparticles for specific applications should be the direct comparison of biological activities with those of alternative materials for the same application. Nanoparticulate inorganic ultraviolet (UV) filters, such as zinc oxide (ZnO), are commonly incorporated into transparent sunscreen and cosmetic formulations. However, concerns have been raised about potential unwanted effects, despite their negligible skin penetration and inherent advantages over organic chemical UV-filters. To provide useful application-relevant assessments of their potential hazard with/without UVA co-exposure, we directly compared cytotoxic and immune response profiles of human THP-1 monocytic cells to ZnO nanoparticles (30 nm) with bulk ZnO particulates (200 nm) and five conventional organic chemical UV-filters - butylmethoxydibenzoylmethane (avobenzone), octylmethoxycinnamate, octylsalicylate, homosalate and 4-methylbenzylidene camphor. High exposure concentrations of both organic and particulate UV-filters were required to cause cytotoxicity in monocyte and macrophage cultures after 24 h. Co-exposure with UVA (6.7 J/cm(2)) did not alter cytotoxicity profiles. Particle surface area-based dose responses showed that ZnO NPs were better tolerated than bulk ZnO. Organic and particulate UV-filters increased apoptosis at similar doses. Only particulates increased the generation of reactive oxygen species. Interleukin-8 (IL-8) release was increased by all particulates, avobenzone, homosalate and octylsalicylate. IL-1β release was only increased in macrophages by exposure to avobenzone and homosalate. In conclusion, direct effects were caused in monocytes and macrophages at similar concentrations of both organic UV-filters and ZnO nanoparticulates - indicating that their intrinsic cytotoxicity is similar. With their lower skin penetration, ZnO nanoparticles are expected to have lower bioactivity when used in sunscreens.

show abstract

Reducing ZnO nanoparticle cytotoxicity by surface modification

Cited by 101 publications

References 49 publications

The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

Biological Reactivity of Zinc Oxide Nanoparticles With Mammalian Test Systems: an Overview

ZnO nanoparticles and organic chemical UV-filters are equally well tolerated by human immune cells

Contact Info

Product

Resources

About