Reducing the Number of UVB Lamps for Low-cost Gel Imaging System Implementation

Abstract

This paper presents a low-cost method of constructing a UV illuminator, which is considered an important component of a gel documentation system. The procedure involves using a smallest-possible UV lamp in the UV illuminator instead of conventional 4 UV lamps.A comparative analysis of images produced by using the commercial gel documentation system and our prototype was performed using MATLAB. Despite the use of only 1 UV lamp, the proposed system demonstrated a similar imaging performance compared to the image quality of a conventional gel documentation system equipped with 4 UV lamps.

. Serotype detection, molecular characterization and genetic relationship study on Pasteurella multocide local isolate

“…PCR condition was programed to pre-denaturation (at 95°C for 15 minutes); denaturation (at 94°C for 1 minute); annealing (at 53°C for 45 seconds); elongation (at 72°C for 1 minute); and postelongation (at 72°C for 10 minutes). The reaction was conducted as 32 cycles and the PCR product was then electrophoresed in the agarose gel 2% at 100 volt, 200 mA for 50 minutes and the desired result was 642 bp under UV exposure or gelDoc camera (Lee at al. 2014).…”