Reducing the nicotine content of tobacco by grafting with eggplant

Ren, Mengjuan; Zhang, Mengyue; Yang, Huijuan; Shi, Hongzhi

doi:10.1186/s12870-020-02459-4

Cited by 9 publications

(7 citation statements)

References 32 publications

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“…The pathways related to the biosynthesis of amino acids and the metabolism of various amino acids have been indicated to be responsible for the degradation of TSNAs [4,7]. In addition, the metabolic 10/18 process of amino acids could reportedly reduce the production of TSNAs by reducing the levels of nitrite [4].…”

Section: Discussionmentioning

confidence: 99%

Combined Transcriptomics and Proteomics Analysis to Reveal the Mechanism underlying the Degradation of TSNAs by Bacillus pumilus 05–5402

Zou,

Lu,

Lei

et al. 2023

Preprint

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Tobacco-specific N-nitrosamines (TSNAs) present in tobacco and tobacco products cause serious diseases and, therefore, effective measures to reduce the levels of TSNAs in tobacco are necessary for tobacco harm reduction. Various bacteria have demonstrated the ability to degrade TSNAs, although their degradation efficiency is often affected by fermentation conditions. Therefore, optimizing the fermentation process is essential to improve the efficiency of the microbial degradation of TSNAs. However, the mechanisms involved in the microbial degradation of TSNAs remain unknown to date. In this context, the present study involved utilizing a combined transcriptomics and proteomics analysis to reveal the mechanism underlying the degradation of TSNAs by Bacillus pumilus strain 05–5402. The TSNAs in flue-cured tobacco were degraded, and the fermentation process was optimized to determine the best conditions for the functioning of strain 05–5402. A TSNA degradation rate of 31.99% was reached at 28°C and a water content of 100%. The combined transcriptomics and proteomics analysis revealed the involvement of diverse mechanisms in the degradation of TSNAs by strain 05–5402, among which amino acid biosynthesis, carbon metabolism, glycolysis/gluconeogenesis, starch and sucrose metabolism, and metabolism of multiple amino acids were the most prominent ones. These findings would serve as a reference for the degradation of TSNAs using Bacillus fermentation at the industrial scale.

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Section: Discussionmentioning

confidence: 99%

Combined Transcriptomics and Proteomics Analysis to Reveal the Mechanism underlying the Degradation of TSNAs by Bacillus pumilus 05–5402

Zou,

Lu,

Lei

et al. 2023

Preprint

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“…The metabolite data highlighted a negative correlation of nicotine to precursors aspartic acid, cadaverine and glutamic acid as well as amino acids glutamine, GABA, threonine, serine, alanine and glycine and polyamine spermidine. Reduction of nicotine content has been previously associated with higher content of precursors (Ren et al, 2020). This implies that more assimilated nitrogen is available for amino acids and subsequently protein biosynthesis in Nicotiana plants with reduced pyridine alkaloid biosynthesis.…”

Section: Metabolites Of Interestmentioning

confidence: 91%

The chemotype core collection of genus Nicotiana

2022

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SUMMARY Sustainable production of chemicals and improving these biosources by engineering metabolic pathways to create efficient plant‐based biofactories relies on the knowledge of available chemical/biosynthetic diversity present in the plant. Nicotiana species are well known for their amenability towards transformation and other new plant breeding techniques. The genus Nicotiana is primarily known through Nicotiana tabacum L., the source of tobacco leaves and all respective tobacco products. Due to the prevalence of the latter, N. tabacum and related Nicotiana species are one of the most extensively studied plants. The majority of studies focused solely on N. tabacum or other individual species for chemotyping. The present study analysed a diversity panel including 17 Nicotiana species and six accessions of Nicotiana benthamiana and created a data set that effectively represents the chemotype core collection of the genus Nicotiana. The utilisation of several analytical platforms and previously published libraries/databases enabled the identification and measurement of over 360 metabolites of a wide range of chemical classes as well as thousands of unknowns with dedicated spectral and chromatographic properties.

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“…Grafting is a widely used agronomic practice that can be applied to improve tobacco yield (Ren et al, 2020 ). Nicotine is synthesized in tobacco roots and transported to leaves, and it has previously been found that the leaves of tobacco grafted onto a tomato rootstock were characterized by no significant accumulation of nicotine (Dawson, 1942 ).…”

Section: Introductionmentioning

confidence: 99%

“…Nicotine is synthesized in tobacco roots and transported to leaves, and it has previously been found that the leaves of tobacco grafted onto a tomato rootstock were characterized by no significant accumulation of nicotine (Dawson, 1942 ). Similarly, the leaves of tobacco that had been grafted onto an eggplant rootstock were found to contain very low levels of nicotine (Ren et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacum L.)

2023

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In this study, we aimed to determine molecular mechanisms underlying the effects of topping and grafting in tobacco ( Nicotiana tabacum L.) by comparing the alkaloid contents and enrichment pathways of differentially expressed genes (DEGs) among plants subjected to different combinations of topping and grafting treatments. Plants of the tobacco variety “Zhongyan 100” and eggplant ( Solanum melongena L.) were grafted in four combinations as scions and rootstocks, respectively. The four treatment groups were tobacco with topping, tobacco without topping, topped tobacco grafted onto an eggplant rootstock, and non‐topped tobacco grafted onto an eggplant rootstock. Tobacco leaves were collected on the day of topping, at 7 days after topping, and after flue curing, the alkaloid contents of the collected leaves were determined. Leaves of plants subjected to the different treatments were collected for RNA sequencing and screened for DEGs, which were subsequently subjected to functional enrichment analyses. Analyses revealed reductions in the leaf alkaloid contents of tobacco subjected to combined topping and eggplant grafting. Gene annotation indicated that topping influences biological processes such as starch metabolism and stress response, whereas grafting affected the biosynthesis and metabolic pathways of secondary metabolites. Downregulated DEGs between non‐topped tobacco and eggplant‐grafted topped tobacco and between topped and non‐topped tobacco are mainly involved in inositol phosphate metabolic and biosynthetic processes. Downregulated DEGs between different grafting methods (eggplant‐grafted non‐topped tobacco vs. non‐topped tobacco and eggplant‐grafted topped tobacco vs. topped tobacco) are mainly involved in sesquiterpene synthase activity and photosynthesis. The findings of this study provide important insights into the molecular mechanisms underlying the effects of topping and grafting on tobacco plants.

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Reducing the nicotine content of tobacco by grafting with eggplant

Cited by 9 publications

References 32 publications

Combined Transcriptomics and Proteomics Analysis to Reveal the Mechanism underlying the Degradation of TSNAs by Bacillus pumilus 05–5402

Combined Transcriptomics and Proteomics Analysis to Reveal the Mechanism underlying the Degradation of TSNAs by Bacillus pumilus 05–5402

The chemotype core collection of genus Nicotiana

Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacum L.)

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