Reducing Severity of Late Blight (Phytophthora infestans) and Improving Potato (Solanum tuberosum L.) Tuber Yield with Pre-Harvest Application of Calcium Nutrients

Seifu, Yewubnesh Wendimu

doi:10.3390/agronomy7040069

Cited by 6 publications

(6 citation statements)

References 25 publications

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“…However, the latter treatment (T 3 ) reduced the overall AUDPC by 81% and increased tuber yield by 264% and 135% in 2017 and 2019, respectively, com- reported that these fungicides can reduce disease severity and increase the potato yield in subtropical plains of India. Additionally, the negative correlation between yield and AUDPC was evident in the present study, and our results are in agreement with earlier studied where different disease management options such as application of fungicides and calcium nutrients, that resulted in reduced late blight disease severity with significant improvement in the yield of crop (Dowley et al, 2008;Seifu, 2017;.…”

Section: Ta B L Esupporting

confidence: 93%

Management of the late blight (Phytophthora infestans) disease of potato in the southern hills of India

Mhatre

Lekshmanan

Palanisamy

et al. 2020

Journal of Phytopathology

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Potato (Solanum tuberosum L.) is the third important food crop in the world (Raymundo et al., 2017) and has the potential to mitigate the challenge of nutritional security. It is a rich source of carbohydrates, dietary fibres, minerals, phytonutrients, proteins, vitamins, etc. and therefore called as 'nutritional depository' (Mishra et al., 2020). In India, potato is being grown in 2.15 million ha with a production of 48.53 million tonnes that accounts for approximately 12% of total world production (FAOSTAT, 2018).

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Section: Ta B L Esupporting

confidence: 93%

Management of the late blight (Phytophthora infestans) disease of potato in the southern hills of India

Mhatre

Lekshmanan

Palanisamy

et al. 2020

Journal of Phytopathology

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“…Disease symptoms started appearing on wild-type plants after ten days postinoculation (10dpi). We analyzed the WT plants for disease rating at 12dpi and observed 5% disease severity according to the scale described by Seifu [29]. Disease on stERF3-loss-of-function mutant plants has appeared on 23dpi, and at 28dpi, disease severity was 2%.…”

Section: Resultsmentioning

confidence: 99%

“…In planta disease assay and quanti cation of fungal biomass Seven-week-old full allelic edited plants and wild-type plants were inoculated with 2×10 4 sporangia ml -1. Disease severity was assessed according to Seifu [29]. For fungal biomass quanti cation, genomic DNA of infected leaves of knockouts and wild type plants was isolated using GeneJET genomic DNA Puri cation Kit (Thermo Scienti c, USA), and real-time qPCR analysis was performed using the primer pair PiO8-3-3Fwd/ PiO8-3-3Rev used by Llorente et al [30] to quantify the Phytophthora infestans biomass in potato.…”

Section: Detached Leaf Assaymentioning

confidence: 99%

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

et al. 2022

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BackgroundThe use of disease-resistant cultivars is the best solution to get their maximum yield potential and avoid fungicide application. There is no doubt about the contribution, and use of R genes (resistance genes) in resistance development in plants, while S genes (susceptibility genes) also hold a strong position in pathogenesis by resistance repression, and their loss of function contributes to enhanced resistance.Hence, we attempted to knock out the function of StERF3 in potatoes through CRISPR/Cas9 based genome editing and investigated the CRISPR/Cas9 approach as strategic control against late blight disease in potato plants. Methods and resultsThe StERF3 gene was edited in late blight susceptible cv. Lady Rosetta. Full allelic edited plants were identi ed through DnpI and N1aIV mediated restriction digestion and then further analyzed through Indel Detection by Amplicon Analysis. Sequence analysis of targeted plants for indel identi cation showed full allelic editing. The detached leaf assay of full allelic edited plants demonstrated the role of the StERF3 gene in susceptibility to late blight in potatoes. In planta disease assay also showed reduced, slowed, and delayed disease progression in StERF3-loss-of-function mutants in comparison to wild-type (control) plants. Less fungal biomass was quanti ed in knockouts through Real-time qPCR that supported less susceptibility of edited plants to late blight. Besides, relatively high expression of pathogens-related genes, StPR1, and StNPR1 were also observed in StERF3-loss-of-function mutants compared to the corresponding control. ConclusionThe results showed the functional inhibition of StERF3 genes using the CRISPR/Cas9 approach. The functional knockouts of StERF3 generated potato plants revealed enhanced resistance against Phytophthora infestans and thereby demonstrated as best strategic control for late blight disease in potato plants.

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“…Optimization of the nutritional status of potato with calcium nutrients can improve tuber yield quality and reduce different diseases [21,23,25,26,30]. Chitosan has a positive effect on the growth and potato tuber yield.…”

Section: Table 2 Influence Of Pre-planting and Foliar Treatment On The Growth And Development Of Potato Plants Infected Withmentioning

confidence: 99%

“…Calcium chloride was reported as a plant resistance inducer and an essential nutritional element in many species, such as tomato against powdery mildew [34], potato against late and early blight [21,23,35]. Arfaoui et al (2016) [36] indicated that pretreatment with calcium enhanced defense responses with higher levels of isoflavone phytoalexins in soybeans, thus reducing infection with Sclerotinia sclerotiorum, and suggesting an indirect impact on the pathogen.…”

Section: Dry Rot Disease Incidencementioning

confidence: 99%

Combined Influence of Chitosan and Calcium Chloride on Fusarium Dry Rot Disease Under Field Conditions

Mohammed¹,

Eskov²,

Zeitar³

2020

TOASJ

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Background: Fusarium dry rot disease caused by Fusarium sambucinum Fuckel (F. sambucinum) can infect the potato tubers in the field and during storage. Yield losses by F. sambucinum reach 60%. Traditional methods to control Fusarium dry rot are fungicides application, which led to developing many isolates resistant to these fungicides. Objective: The aim of this study is to evaluate the effect of calcium chloride (CaCl2) and chitosan, alone or in combination, on plant development, tuber yield, and Fusarium dry rot disease incidence under field conditions. Methods: Soil inoculated with F. sambucinum before planting. We treated the seed tubers with CaCl2 (0.5 or 1%), chitosan 0.5%, or both. The foliage was sprayed twice with CaCl2 (0.5 or 1%), 0.1% chitosan, or both. During the vegetation period, growth parameters, such as germination (%), plant height (cm), and branches number per plant, were measured. At harvest, we calculated the total and the marketable number of tubers and tuber yield. In addition, during storage, we assessed the incidence of Fusarium dry rot disease on tubers. Results: Results revealed that combined pre-planting application with 1% CaCl2 and 0.5% chitosan with 2 hours intervals, then spraying foliar with 1% CaCl2 and 0.1% chitosan twice with ten days intervals starting at 40 days after planting resulted in: a) increasing the germination, enhancing the growth parameters such as plant height and branches number per plant; b) enhancing the marketable tuber yield by 75.2 and 97.6% in Sante and Kolobok varieties, respectively; c) reducing Fusarium dry rot disease incidence by 61.9-72.7%. Conclusion: The work highlighted that the combined pre-planting and foliar application of CaCl2 and chitosan might be recommended for potato producers to reduce the incidence of Fusarium dry rot disease and augment yields.

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Reducing Severity of Late Blight (Phytophthora infestans) and Improving Potato (Solanum tuberosum L.) Tuber Yield with Pre-Harvest Application of Calcium Nutrients

Cited by 6 publications

References 25 publications

Management of the late blight (Phytophthora infestans) disease of potato in the southern hills of India

Management of the late blight (Phytophthora infestans) disease of potato in the southern hills of India

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

Combined Influence of Chitosan and Calcium Chloride on Fusarium Dry Rot Disease Under Field Conditions

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