Reducing electrospun nanofiber diameter and variability using cationic amphiphiles

Lin, Kenneth; Chua, Koon Jiew; Christopherson, Gregory T.; Lim, Shawn; Mao, Hai‐Quan

doi:10.1016/j.polymer.2007.08.056

Cited by 72 publications

(53 citation statements)

References 28 publications

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“…This phenomenon may be due to the comprehensive effects of the surface tension, solution conductivity and viscosity. [31] The results from Figure 3 indicate that the surface tension is likely to be the primary factor that contributes to regulating the morphology of the fibers and nano-nets.…”

Section: Resultsmentioning

confidence: 96%

One‐step Electro‐spinning/netting Technique for Controllably Preparing Polyurethane Nano‐fiber/net

Wang

Ding

et al. 2011

Macromol. Rapid Commun.

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Electro-spinning/netting (ESN) as a cutting-edge technique evokes much interest because of its ability in the one-step preparation of versatile nano-fiber/net (NFN) membranes. Here, a controllable fabrication of polyurethane (PU) NFN membranes with attractive structures, consisting of common electrospun nanofibers and two-dimensional (2D) soap bubble-like structured nano-nets via an ESN process is reported. The unique nanoscaled NFN architecture can be finely controlled by regulating the solution properties and several ESN process parameters. The versatile PU nano-nets comprising interlinked nanowires with ultrathin diameters (5-40 nm) mean that the NFN structured membranes possess several excellent characteristics, such as an extremely large specific surface area, high porosity and large stacking density, which would be particularly useful for applications in ultrafiltration, special protective clothing, ultrasensitive sensors, catalyst support and so on.

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Section: Resultsmentioning

confidence: 96%

One‐step Electro‐spinning/netting Technique for Controllably Preparing Polyurethane Nano‐fiber/net

Wang

Ding

et al. 2011

Macromol. Rapid Commun.

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“…Bead formation occurred as a result of the different evaporation time and surface tension of the mixed polymer solution. [38][39][40] Therefore, our nonhomogeneity of fiber diameters may be due to our use of both low polymer concentrations and co-solvents. However, our γ-ray surface modification process used for RGD peptide immobilization did not affect the structure of fibrous meshes, indicating that it is an appropriate method to modify the surface of electrospun fibrous meshes.…”

Section: Resultsmentioning

confidence: 99%

Surface modification of electrospun poly(L-lactide-co-ɛ-caprolactone) fibrous meshes with a RGD peptide for the control of adhesion, proliferation and differentiation of the preosteoblastic cells

Shin

Lim

2010

Macromol. Res.

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Regulation of cell-material interactions is an important factor for modulating the cell function in many tissue engineering applications. A more attractive strategy for enhancing the cell-material interactions is to mimic the physical and chemical features of the native extracellular matrix (ECM). The main goal of this study was to develop ECM-like substrates that can control the cell-material interactions including adhesion, spreading, proliferation and differentiation. Poly(L-lactide-co-ε-caprolactone) (PLCL) fibrous meshes were fabricated using electrospinning. The meshes were functionalized with acrylic acid (AAc) using γ-ray irradiation, and Arg-Gly-Asp (RGD)-containing peptide was immobilized on the resulting mesh as a cell adhesive ligand. The adhesion and proliferation of the MC3T3-E1 pre-osteoblastic cells grown on the RGD-AAc-PLCL fibrous meshes were greater than those of the cells grown on the other fibrous meshes for up to 7 days. In addition, mature formation of F-actin stress fibers and focal adhesion (co-localized with vinculin) was only observed on the RGD-AAc-PLCL meshes. Moreover, the ALP activity and calcium content on the RGD-AAc-PLCL meshes were approximately 7.5 and 6.7 times higher than those on the other meshes, respectively. In addition, the expression of selected osteogenic genes, Cbfa1, ALP, and OCN, was significantly up-regulated (at least 5 to 9.7 times greater) on the RGD-AAc-PLCL meshes. This suggests that peptide-modified fibrous meshes eliciting desirable cellular responses may provide a useful tool for many tissue engineering applications.

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“…Natural, synthetic, and composite polymeric materials are electrospun to obtain fibers, and the diameter of the fibers can be adjusted by varying the polymer concentrations, solvents used, or by modulating the spinning conditions [15,16]. Electrospun nanofibers provide a high surface area for cell attachment, and functionalization of fibers is possible by chemical conjugation of ECM molecules or by protein coatings (composite scaffolds).…”

Section: Nanostructure Fabrication Methodsmentioning

confidence: 99%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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Stem cells are a promising alternative for cell therapy applications because of their self-renewing capability and potential to differentiate into a wide range of specialized cell lineages, including osteoblasts, chondrocytes, cardiomyocytes, and neuronal cells. Different kinds of stem cells are considered for cell-based tissue engineering approaches, of which bone-marrow-derived mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs) and embryonic stem cells (ESCs) are frequently utilized for advancement of new tissue engineering strategies. Nanostructures created by natural, synthetic, or composite polymeric biomaterials provide artificial templates of the extracellular matrix (ECM). They possess a high surface area to volume ratio, are porous with good mechanical properties, are sufficient to serve as a biomimetic platform to attract stem cells, cause differentiation, and provide functioning of the tissues. Nanoscale features such as fibers, pits, and grooves modulate cell behavior and might even stimulate the differentiation of stem cells to specific lineages; for example, the nanotopographical cues combined with chemical cues influence the neuronal induction of MSCs, resulting in high microtubule-associated protein expressions. Scaffolds can also be impregnated with several ligands for adhesion of receptors to the cells, or with other regulatory molecules and growth M.P. Prabhakaran (*) and J. Venugopal E3-05-12, Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore e-mail: nnimpp@nus.edu.sg L. Ghasemi-Mobarakeh Islamic Azad University, Najafabad Branch, Isfahan, Iran D. Kai NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore G. Jin and S. Ramakrishna Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore factors for designed cell behavior or controlled cell differentiations. Different polymeric blends or fibers incorporated with nanoparticles (such as hydroxyapatite) in aligned or custom-made patterns can induce specific differentiation of stem cells for bone, cartilage, cardiac, nerve, and skin tissue regeneration. In this review, we will discuss the current state of the art and future perspectives on stem cells and their differentiation on nanoengineered substrates for advanced tissue regeneration.

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Reducing electrospun nanofiber diameter and variability using cationic amphiphiles

Cited by 72 publications

References 28 publications

One‐step Electro‐spinning/netting Technique for Controllably Preparing Polyurethane Nano‐fiber/net

One‐step Electro‐spinning/netting Technique for Controllably Preparing Polyurethane Nano‐fiber/net

Surface modification of electrospun poly(L-lactide-co-ɛ-caprolactone) fibrous meshes with a RGD peptide for the control of adhesion, proliferation and differentiation of the preosteoblastic cells

Stem Cells and Nanostructures for Advanced Tissue Regeneration

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