Reducing Cellular Autolysis of Bacillus subtilis to Improve Keratinase Production
Xiwen Chen,
Zheng Peng,
Xiaomei Ji
et al.
Abstract:Bacillus subtilis has
been shown
to be an excellent expression host for keratinases due to its powerful
secretion system. However, cellular autolysis limits its production
capacity. Here, we selected seven genes with significantly upregulated
transcript levels from 15 genes associated with cellular autolysis
as knockout targets by qRT-PCR and constructed a total of 127 strains
to reduce cellular autolysis. Among them, the biomass of B. subtilis BSΔXLPC-ker deficient
in xpf, lytC, pcfA, and cwlC increased by 57… Show more
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