Reducing aflatoxin toxicity of contaminted diet fed to guinea pigs by addition of Egyptian Tafla

Abstract

Effect of Egyptian Tafla on reducing the toxicity of aflatoxin was examined by adding different levels of Tafla to an aflatoxincontaminated diet fed to 30 growing male guinea pigs (average 133 gm). The animals divided into 5 groups equal in number and average body weight. Five diets were prepared uncontaminated diet (control), contaminated diet with aflatoxins Bt, B2, GI and G2 mixture (equals to 420 ppb aflatoxin B I), aflatoxins contaminated diet supplied with 1/2, 1 or 2% Tafla. Each of the 5 animal groups was fed with one diet only. The following measurement were recorded, daily feed intake, daily body weight gain, feed efficiency, aflatoxin intake (ppb). On the 17 th day of feeding period, three animals from each group were slaughtered. Blood samples were taken individually for biochemical study. Internal organs of each animal (liver, lung, kidney, heart and spleen) were clinically examined, weighed and samples for histopthological studies were taken. The obtained results revealed that comparing to the uncontaminated (control) diet, feeding aflatoxins B1, B2, GI and G2 mixture (equal to 420 ppb as BI) contaminated diet caused. Reduce in each of live body weight, body gain, feed intake and feed efficiency. Decrease in absolute weights of internal organs (significant only with liver and spleen) and significant increase in its relative weights. Decrease in serum total protein, ablumin and globulin, meanwhile an increase in serum total lipids, total cholesterol, creatinine, urea-N, uric acid and GOT, GPT and alkaline phosphatase activities. The differences were significant (P<0.05) except in serum albumin, and urea-N. Addition of 1 /2, I and 2% Tafla to the aflatoxins diet caused, minimize the losses in body weight and increased daily feed intake and prolonged the survival period of tested animals. Minimize the changes in blood serum components. Where the significant (P<0.05) decrease in serum globulin, cholesterol, and GOT, GPT and alkaline phosphatase activities become insignificant. However the hazard effect of aflatoxins ingestion was minimized by addition of Tafla (especially the 1 /2% level). The differences in serum creatinine and uric acid still significant comparing to the control diet. Minimized the hazard effect on liver and kidney functions. The 1 /2% level of Tafla was the highest efficient in detoxification of aflatoxins and reducing its toxicity.