Reducible poly(amido ethylenimine) directed to enhance RNA interference

Jeong, Ji Hoon; Christensen, Lane V.; Yockman, James W.; Zhong, Zhiyuan; Engbersen, Johan F.J.; Kim, Won Jong; Feijén, Jan; Kim, Sung Wan

doi:10.1016/j.biomaterials.2006.12.019

Cited by 163 publications

(47 citation statements)

References 24 publications

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“…A reducible poly(amido ethylenimine) (SS-PAEI) synthesized by addition copolymerization of triethylenetetramine and cystamine bis-acrylamide (poly(TETA/CBA)) was used as a carrier for siRNA (55) (Fig. 2).…”

Section: Cationic Polymeric Delivery Systemsmentioning

confidence: 99%

Efficient siRNA Delivery with Non-viral Polymeric Vehicles

2008

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Sequence-specific gene silencing using small interfering RNA (siRNA) provides a potent and specific method for gene expression, thus is now being evaluated in clinical trials as a novel therapeutic strategy. As a results, there has been a significant surge of interest in the application of siRNA in therapeutics as a means of silencing the specific gene function. However, for siRNA technology to be valuable and effective, the development of efficient siRNA delivery strategy is essential for improving biological activities such as stability, cellular uptake, sequence-specificity, devoid of nonspecific knockdown and toxic side effects. Accordingly, a number of delivery systems, both viral and nonviral, have been reported and some of them successfully used for the introduction of siRNA into cells both in vitro and in vivo. Here, we discuss the current understanding of synthetic siRNA delivery mechanism and strategies of siRNA delivery by non-viral polymeric vehicles which are currently used in vitro and in vivo.

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Section: Cationic Polymeric Delivery Systemsmentioning

confidence: 99%

Efficient siRNA Delivery with Non-viral Polymeric Vehicles

2008

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“…However, excess positive charge is associated with tissue and cellular toxicity (Morgan et al, 1989;Hoon Jeong et al, 2007). To develop a new gene carrier with high uptake efficacy and low-cytotoxicity, we synthesized a bio-reducible carrier, STR-CH 2 R 4 H 2 C, that can form a stable complex with nucleic acids in the non-reducing extracellular envi-0378-5173/$ -see front matter © 2010 Elsevier B.V. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

Disulfide crosslinked stearoyl carrier peptides containing arginine and histidine enhance siRNA uptake and gene silencing

Tanaka

Kanazawa

Ogawa

et al. 2010

International Journal of Pharmaceutics

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“…Especially the class of biodegradable and biocompatible polymers (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) has gained more and more interest during the last years. PEGylation of polycations is a common strategy to further improve biocompatibility of polyplexes (19,20) but has the drawback of reducing transfection efficiency at the same time.…”

Section: Discussionmentioning

confidence: 99%

“…Recent biodegradable polymer systems (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) are trend-setting, as they show much better biocompatibility than non-degradable analogs. Most recently we reported the generation of novel acetal linked oligoethylenimines that may undergo acid-catalyzed hydrolysis in the endosome (16).…”

Section: Introductionmentioning

confidence: 99%