Reduced secretion of IgA to skin surface of patients with atopic dermatitis

Imayama,; Shimozono,; Hoashi,; Yasumoto,; Ohta,; Yoneyama,; Hori,

doi:10.1053/ai.1994.v94.a55222

Cited by 61 publications

(12 citation statements)

References 28 publications

(16 reference statements)

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“…Accordingly, AD patients show significantly lower sweat rates upon moderate thermal stress (29). Differences in the composition of sweat, with reduced secretion of IgA (30) and altered sweat electrolyte concentrations (31) in children with AD, have been described. Therefore, our findings underline specific differences in sweating that may be related to the high incidence of skin infections.…”

Section: Discussionmentioning

confidence: 99%

Deficiency of Dermcidin-Derived Antimicrobial Peptides in Sweat of Patients with Atopic Dermatitis Correlates with an Impaired Innate Defense of Human Skin In Vivo

Rieg

Steffen²,

Seeber³

et al. 2005

The Journal of Immunology

249

185

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Antimicrobial peptides are an integral part of the epithelial innate defense system. Dermcidin (DCD) is a recently discovered antimicrobial peptide with a broad spectrum of activity. It is constitutively expressed in human eccrine sweat glands and secreted into sweat. Patients with atopic dermatitis (AD) have recurrent bacterial or viral skin infections and pronounced colonization with Staphylococcus aureus. We hypothesized that patients with AD have a reduced amount of DCD peptides in sweat contributing to the compromised constitutive innate skin defense. Therefore, we performed semiquantitative and quantitative analyses of DCD peptides in sweat of AD patients and healthy subjects using surface-enhanced laser desorption ionization time-of-flight mass spectrometry and ELISA. The data indicate that the amount of several DCD-derived peptides in sweat of patients with AD is significantly reduced. Furthermore, compared with atopic patients without previous infectious complications, AD patients with a history of bacterial and viral skin infections were found to have significantly less DCD-1 and DCD-1L in their sweat. To analyze whether the reduced amount of DCD in sweat of AD patients correlates with a decreased innate defense, we determined the antimicrobial activity of sweat in vivo. We showed that in healthy subjects, sweating leads to a reduction of viable bacteria on the skin surface, but this does not occur in patients with AD. These data indicate that reduced expression of DCD in sweat of patients with AD may contribute to the high susceptibility of these patients to skin infections and altered skin colonization.

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Section: Discussionmentioning

confidence: 99%

Deficiency of Dermcidin-Derived Antimicrobial Peptides in Sweat of Patients with Atopic Dermatitis Correlates with an Impaired Innate Defense of Human Skin In Vivo

Rieg

Steffen²,

Seeber³

et al. 2005

The Journal of Immunology

249

185

View full text Add to dashboard Cite

show abstract

“…The present study confirms that the skin surface of patients with AD is widely colonized by a higher number of microbial populations, particularly of S. aureus , than that of control patients (Table 2). 12 Existence of higher population levels of bacteria on atopic skin may be caused by decreasing sphingomyelinase and ceramide, 13 reduced secretion of IgA, high pH and salt‐rich sweat 14 . Williams et al .…”

Section: Discussionmentioning

confidence: 99%

Influence of narrow‐band UVB phototherapy on cutaneous microbiota of children with atopic dermatitis

Silva¹,

Guedes

Gontijo

et al. 2006

Acad Dermatol Venereol

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“…The patient was diagnosed according the clinical criteria (18,19), past history of other atopic disease, high total IgE level (22,100 units/ml), and typical eczematous skin lesions on the face, neck, and extremities.…”

Section: Materials-mentioning

confidence: 99%

Purification and Characterization of a Novel Ceramidase fromPseudomonas aeruginosa

Okino

Tani

Imayama

et al. 1998

Journal of Biological Chemistry

103

117

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We report here a novel type of ceramidase of Pseudomonas aeruginosa AN17 isolated from the skin of a patient with atopic dermatitis. The enzyme was purified 83,400-fold with an overall yield of 21.1% from a culture supernatant of strain AN17. After being stained with a silver staining solution, the purified enzyme showed a single protein band, and its molecular mass was estimated to be 70 kDa on SDS-polyacrylamide gel electrophoresis. The enzyme showed quite wide specificity for various ceramides, i.e. it hydrolyzed ceramides containing C12:0 -C18:0 fatty acids and 7-nitrobenz-2-oxa-1,3-diazole-labeled dodecanoic acid, and not only ceramide containing sphingosine (d18:1) or sphinganine (d18:0) but also phytosphingosine (t18:0) as the long-chain base. However, the enzyme did not hydrolyze galactosylceramide, sulfatide, GM1, or sphingomyelin, and thus was clearly distinguished from a Pseudomonas sphingolipid ceramide N-deacylase (Ito, M., Kurita, T., and Kita, K.

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Reduced secretion of IgA to skin surface of patients with atopic dermatitis

Cited by 61 publications

References 28 publications

Deficiency of Dermcidin-Derived Antimicrobial Peptides in Sweat of Patients with Atopic Dermatitis Correlates with an Impaired Innate Defense of Human Skin In Vivo

Deficiency of Dermcidin-Derived Antimicrobial Peptides in Sweat of Patients with Atopic Dermatitis Correlates with an Impaired Innate Defense of Human Skin In Vivo

Influence of narrow‐band UVB phototherapy on cutaneous microbiota of children with atopic dermatitis

Purification and Characterization of a Novel Ceramidase fromPseudomonas aeruginosa

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