Reduced reperfusion injury by glycine in a porcine liver transplantation model with non–heart-beating donors

Barros-Schelotto, P.; Net, M.; Valero, Ricard; Ruíz, Ángel; Almenara, R; Capdevila, Lluís; Sugrañes, Gemma; Suarez-Crivaro, F; López-Boado, M.Á.; Pellegrino, Arine; Deulofeu, R.; Miquel, Rosa; Taurà, Pilar; Manyalich, M.; García‐Valdecasas, Juan Carlos

doi:10.1016/s0041-1345(02)02636-2

Cited by 10 publications

(6 citation statements)

References 11 publications

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“…In particular, studies have focused on the mechanism of ischaemia‐reperfusion injury, and several studies have suggested that glycine protects kidney tubules from ischaemic injury both by in vivo and in vitro experiments 21‐23 . Furthermore, glycine showed a protective effect in transplantation organ storage and perfusion 24,25 . Even some clinical trials showed that glycine administration was associated with improved liver function parameters after liver transplantation, and a trial showed that glycine administration accelerated urinary uric acid excretion 26,27 .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive metabolomic profiling in early IgA nephropathy patients reveals urine glycine as a prognostic biomarker

Park

Lee

Yang

et al. 2021

J Cellular Molecular Medi

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Section: Discussionmentioning

confidence: 99%

Comprehensive metabolomic profiling in early IgA nephropathy patients reveals urine glycine as a prognostic biomarker

Park

Lee

Yang

et al. 2021

J Cellular Molecular Medi

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“…They administered glycine as part of in-situ NR, for 15 minutes, after 40 minutes warm ischemia. They found significantly better hepatic arterial and portal flow during NR, and increased hyaluronic acid uptake (a marker of endothelial cell function), reduced ischemic necrosis and improved survival in the group administered glycine [88]. Rentsch et al, have shown more perfused sinusoids and less white blood cell infiltration in livers obtained from donor rats administered glycine.…”

Section: Experience With Animal Liver Trans-plantationmentioning

confidence: 96%

The Role of Glycine in Hepatic Ischemia-Reperfusion Injury

Habib¹,

Hodgson²,

Davidson

2006

CPD

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Glycine is a non-essential amino acid which is cheap, easily available and relatively non-toxic. It is composed of a single carbon attached to an amino and a carboxyl group, with a molecular weight of 75. It is involved in the production of bile, nucleic acids, porphyrins and creatine phosphate. It is part of the normal human diet and is used clinically, as an irrigant solution in urological and gynaecological procedures. Glycine has broad spectrum anti-inflammatory, cytoprotective and immunomodulatory properties whose therapeutic role has largely been un-investigated. Since the demonstration of its cytoprotective effect on hypoxic cultured renal tubule cells, further research has established its mechanism of anti-inflammatory action, which depends on stimulation of glycine sensitive chloride channel receptors on the cell membrane. The mechanism of non-specific cytoprotective effect which is present even in chloride and calcium free media is not clear. However glycine is currently being used experimentally, in human liver transplant recipients and has been shown to be beneficial in animal models of ischemia-reperfusion injury (IRI) in liver and several other organs. This review addresses the properties of glycine, its mechanism of action and its role in modulating IRI with special reference to the liver, with the aim of stimulating translational research into the potential role of glycine as a pharmaceutical agent.

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“…Again in the rat, rinsing of the transplants prior to implantation with Carolina rinse solution additionally containing 5 mM glycine largely prevented liver injury following transplantation (Bachmann et al ., 1995). In a pig model of a non‐heart‐beating donor, inclusion of 25 mg glycine per kilogram during normothermic reperfusion following warm ischaemia (still within the donor using a heart lung machine) decreased liver injury and improved outcome following transplantation (Barros‐Schelotto et al ., 2002). In dog liver transplantation experiments, glycine was not protective when included in the UW solution during cold preservation (see above).…”

Section: Protection By Glycine In Vivomentioning

confidence: 99%

Glycine, a simple physiological compound protecting by yet puzzling mechanism(s) against ischaemia–reperfusion injury: current knowledge

Petrat

Boengler

Schulz

et al. 2012

British J Pharmacology

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Ischaemia is amongst the leading causes of death. Despite this importance, there are only a few therapeutic approaches to protect from ischaemia-reperfusion injury (IRI). In experimental studies, the amino acid glycine effectively protected from IRI. In the prevention of IRI by glycine in cells and isolated perfused or cold-stored organs (tissues), direct cytoprotection plays a crucial role, most likely by prevention of the formation of pathological plasma membrane pores. Under in vivo conditions, the mechanism of protection by glycine is less clear, partly due to the physiological presence of the amino acid. Here, inhibition of the inflammatory response in the injured tissue is considered to contribute decisively to the glycine-induced reduction of IRI. However, attenuation of IRI recently achieved in experimental animals by low-dose glycine treatment regimens suggests additional/other (unknown) protective mechanisms. Despite the convincing experimental evidence and the large therapeutic width of glycine, there are only a few clinical trials on the protection from IRI by glycine with ambivalent results. Thus, both the mechanism(s) behind the protection of glycine against IRI in vivo and its true clinical potential remain to be addressed in future experimental studies/clinical trials.

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Reduced reperfusion injury by glycine in a porcine liver transplantation model with non–heart-beating donors

Cited by 10 publications

References 11 publications

Comprehensive metabolomic profiling in early IgA nephropathy patients reveals urine glycine as a prognostic biomarker

Comprehensive metabolomic profiling in early IgA nephropathy patients reveals urine glycine as a prognostic biomarker

The Role of Glycine in Hepatic Ischemia-Reperfusion Injury

Glycine, a simple physiological compound protecting by yet puzzling mechanism(s) against ischaemia–reperfusion injury: current knowledge

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