Reduced RCE1 expression predicts poor prognosis of colorectal carcinoma

Abstract

BackgroundAs an end-proteolytic enzyme that cleaves the last three residues of proteins with a terminal CAAX, Ras-converting enzyme 1 (RCE1) has an essential role in multiple signaling pathways and take part in the process of differentiation, proliferation and carcinogenesis. The aim of the study is to investigate expression pattern of RCE1 and its prognosis in colorectal carcinoma (CRC).MethodsThe expression of RCE1 and phospho-MAPK family members was confirmed by immunohistochemical staining of CRC tissues. miR-RCE1 lentiviral vectors were transduced into HCT116 and SW489 cells. Reverse transcription PCR (RT-PCR) and western blot were conducted to measure the transfection efficiency. Transwell assays were used to detect the invasiveness of CRC cells.ResultsIn the present study, we assessed RCE1 expression in 244 CRC specimens and matching adjacent, non-tumorous tissues by immunohistochemistry (IHC). Compared with the matched adjacent non-tumor tissue samples, the RCE1 reduced in the tumor tissue samples (p < 0.001). RCE1 expression was significantly decreased in 106 of 244 (43.4%) CRC cases. In univariate and multivariate analyses, Decreasing expression of RCE1 independently predicts poor prognosis for patients in both overall survival and disease-free survival. Further study indicated that RCE1 influenced tumor invasion through the p38 pathway. Knockdown of RCE1 reduced phosphorylation and significantly increased the invasive capacity of CRC cells.ConclusionTaken together, the outcomes of this study indicate that RCE1 acts as a tumor suppressor in CRC, as its reduced expression may increase CRC cell invasion and independently predict an unsatisfactory prognosis in CRC patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-017-3393-3) contains supplementary material, which is available to authorized users.

Rce1 suppresses invasion and metastasis of hepatocellular carcinoma via epithelial‐mesenchymal transition induced by the TGF‐β1/H‐Ras signaling pathway

“…Research works have shown that Rce1 plays a crucial role in the cancerogenesis and development of various malignant tumors, such as renal cell carcinoma (J. Li et al, 2017), prostate cancer (Huang et al, 2016), and colorectal cancer (Shi et al, 2017). At present, Rce1 has not been studied in HCC and the specific molecular mechanism of Rce1 in the early invasion and metastasis remains unclear.…”

“…Rce1 participates in posttranslational modification of Ras in the endoplasmic reticulum and undergoes subsequent methylation and additional transcription processing (Jaworski et al, ; Stout & Campbell, ). Research works have shown that Rce1 plays a crucial role in the cancerogenesis and development of various malignant tumors, such as renal cell carcinoma (J. Li et al, ), prostate cancer (Huang et al, ), and colorectal cancer (Shi et al, ). At present, Rce1 has not been studied in HCC and the specific molecular mechanism of Rce1 in the early invasion and metastasis remains unclear.…”

Rce1: mechanism and inhibition

“…The development and testing of RPIs and MTIs substantially lag that of prenyl transferase inhibitors. Rce1 is also being investigated as a potential biomarker in various types of cancer, but its utility as a disease predictor remains far from being understood ( Huang et al 2016 ; Li et al 2017 ; Shi et al 2017 ).…”

LNP-miR-155 cy5 Inhibitor Regulates the Copper Transporter via the β-Catenin/TCF4/SLC31A1 Signal for Colorectal Cancer Therapy

“…Colorectal carcinoma is one of the most common types of cancer that causes over 600,000 cancer-associated deaths each year worldwide . Despite numerous advances in modern diagnostic and therapeutic techniques, the clinical prognosis of colorectal carcinoma patients remains poor . Therefore, it is of great significance to identify targeted therapeutic drugs for early diagnosis and clinical treatment of colorectal carcinoma.…”