Thanks to God for all the blessings he has given me in my life. I would like to thank my adviser Dr. Greg Clement for his unconditional support, guidance, encouragement, and patience throughout my PhD program. Without his support this project would not have happened. Upon his departure from the lab, Dr. Aaron Fleischman took over in an advisory role and I must thank him for bringing added detail and closure to my project. Dr. Margot Damaser took a chance on me by hiring me to work in her lab while I was still finishing this project, and I could not be more grateful that she did. She was very supportive and understanding about my obligations and gave me a great opportunity to succeed in the next chapter of my life. I would also like to thank my academic adviser, Dr. Dan Simon and his wife, Annette for their support and encouragement. While I was trying hard to succeed in my program, I faced many challenges. Dr. Simon was always there for me as an instructor, mentor, and confidant. I could not overcome my challenges without his support. I would like to thank all of my thesis committee members. During my program, I had an opportunity to work at Cleveland Clinic, Dr. Clement ultrasound lab. The lab manager, Mark Howell, had pivotal role in my success. I would like to thank him for all he has done for me. He is a great friend and a lab partner. Last but not least, I would like to thank my family for their unconditional support throughout my time at CSU. In particular, my mum, Tooran, my dad, Ali and my dear brother, Dr. Abbas Smiley.