Reduced prevalence of genotype 3 HEV in Shanghai pig farms and hypothetical homeostasis of porcine HEV reservoir

Li, Zhen; Yu, Shuisheng; Dong, Shijuan; Zhu, Yumin; Si, Fusheng; Shi-yuan, Shen; Zhong-qi, Jiang; Yu, Ran; Zou, Shanmei

doi:10.1016/j.vetmic.2008.12.013

Cited by 12 publications

(11 citation statements)

References 22 publications

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“…Nonetheless, both scenarios are consistent with HEV infection through consumption of figatellu, recently reported to be a potential source of HEV if eaten uncooked [10]. Indeed, different HEV genotypes and subtypes have previously been recovered from pigs in the same farm, and co-infection of a pig with two different genotypes was also observed [13]. In addition, both present HEV 3c and 3e sequences were nearly identical to the sequences of two viruses recovered from figatelli bought at a local supermarket [10].…”

Section: Case Presentationsupporting

confidence: 77%

First Human Case of Co-Infection with Two Different Subtypes of Hepatitis E Virus

2012

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Hepatitis E virus (HEV), the main etiologic agent of enterically transmitted acute hepatitis in developing countries, is now recognized as an emerging agent of autochthonous disease and chronic hepatitis E in immunocompromised patients in Europe where HEV infection is probably zoonotically acquired. We describe the first human case of acute HEV infection with two genotype 3 viruses in a French kidney transplant recipient, probably acquired through consumption of uncooked pig liver sausage (figatellu). The patient presented two viral sequences nearly identical to sequences recovered from figatelli. Autochthonous co-infections with different genotype 3 HEV strains can occur in our geographical area.

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Section: Case Presentationsupporting

confidence: 77%

First Human Case of Co-Infection with Two Different Subtypes of Hepatitis E Virus

2012

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“…Anti-HEV IgM antibody levels appear to be age-dependent, with the highest proportion found in young pigs (up to three months old) in response to their first contact with the virus, which usually occurs soon after weaning [20,[35][36][37][38]. The samples analyzed in the present study were obtained from pigs at slaughter age (approximately six months), what may have led to the failure in detecting IgM antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…In this pig, anti-HEV IgG was detected at low levels (25.6 U/mL) and HEV RNA was detected in its serum, feces and liver samples. Considering the possibility that the virus can be maintained among infected populations, this animal may represents a case of re-infection [37]. Twelve (7.9%; 12/151) seropositive animals in which HEV RNA was not detected appear to represent cases of previous infection.…”

Section: Discussionmentioning

confidence: 99%

HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes

Souza¹,

Gomes‐Gouvêa²,

Soares³

et al. 2012

Comparative Immunology, Microbiology and Infectious Diseases

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“…Fecal and sera samples were tested for genotype 3 HEV RNA and HEV antibodies using RT-nPCR and ELISA (Wantai Biopharmaceutical, Inc., Beijing, China), respectively [47]. All assay procedures were carried out according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Construction of an Infectious cDNA Clone of a Swine Genotype 3 HEV Strain Isolated in Shanghai, China

Shi²,

Wang³

et al. 2014

Intervirology

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Objectives: Infectious cDNA clones are important tools for studying molecular mechanisms in RNA viruses. The aim of this study was to construct an infectious cDNA clone for SAAS-JDY5, which is a genotype 3 HEV strain of swine origin. Methods: Construction employed overlapping PCR and restriction analysis to ligate nine cDNA fragments into a full-length cDNA clone containing 14 mutations compared to the consensus HEV genome sequence. Megaprimer PCR-directed mutagenesis restored nine non-silent mutations back to the consensus sequence while the other five silent mutations were maintained as genetic markers. Results: HEV proteins were identified by an immunofluorescence assay in Huh7 cells infected with capped RNA transcripts of the full-length cDNA clone, while HEV viremia, fecal HEV RNA and seroconversion were recorded in inoculated Sprague-Dawley rats. Conclusions: Our data confirmed the successful construction of an infectious cDNA clone of swine HEV strain pGEM4z-SAAS-JDY5, and support the use of rats as an HEV infectious model.

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Reduced prevalence of genotype 3 HEV in Shanghai pig farms and hypothetical homeostasis of porcine HEV reservoir

Cited by 12 publications

References 22 publications

First Human Case of Co-Infection with Two Different Subtypes of Hepatitis E Virus

First Human Case of Co-Infection with Two Different Subtypes of Hepatitis E Virus

HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes

Construction of an Infectious cDNA Clone of a Swine Genotype 3 HEV Strain Isolated in Shanghai, China

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