Reduced postoperative intra-abdominal adhesions using Carbylan-SX, a semisynthetic glycosaminoglycan hydrogel

Liang, Yanchun; Shu, Xiao Zheng; Prestwich, Glenn D.

doi:10.1016/j.fertnstert.2006.07.1532

Cited by 67 publications

(48 citation statements)

References 47 publications

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“…Its preparation protocol is very "user-friendly" and suitable for large-scale experimental protocols, its gelation time can be adjusted by varying pH or temperature, and its compliance can be altered by adjusting the degree of cross-linking [44]. In addition, its nature overcomes the issue of immunogenicity in in vivo applications [27][28][29][30][31][32][33]. In this study, the cellular morphology in 3-D encapsulated Extracel ™ was mostly rounded rather than the stretched, spindle-shape appearance seen in other scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…Another ECM equivalent, Extracel ™ , consists of a chemically modified hyaluronan (CMHA-S, also known as Carbylan ™ -S) and gelatin (Gtn-DTPH), which are co-cross-linked with polyethylene glycol diacrylate (PEGDA) [24,25]. This synthetic ECM (sECM) has been used successfully in numerous tissue engineering applications, has broad applicability in drug discovery [26], and is suitable for both 3-D and pseudo-3-D plating [27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Effects of extracellular matrix analogues on primary human fibroblast behavior

2008

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In vitro cell culture is a vital research tool for cell biology, pharmacology, toxicology, protein production, systems biology and drug discovery. Traditional culturing methods on plastic surfaces do not accurately represent the in vivo environment, and a paradigm shift from two-dimensional to three-dimensional (3-D) experimental techniques is underway. To enable this change, a variety of natural, synthetic and semi-synthetic extracellular matrix (ECM) equivalents have been developed to provide an appropriate cellular microenvironment. We describe herein an investigation of the properties of four commercially available ECM equivalents on the growth and proliferation of primary human tracheal scar fibroblast behavior, both in 3-D and pseudo-3-D conditions. We also compare subcutaneous tissue growth of 3-D encapsulated fibroblasts in vivo in two of these materials, Matrigel ™ and Extracel ™ . The latter shows increased cell proliferation and remodeling of the ECM equivalent. The results provide researchers with a rational basis for selection of a given ECM equivalent based on its biological performance in vitro and in vivo, as well as the practicality of the experimental protocols. Biomaterials that use a customizable glycosaminoglycan-based hydrogel appear to offer the most convenient and flexible system for conducting in vitro research that accurately translates to in vivo physiology needed for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of extracellular matrix analogues on primary human fibroblast behavior

2008

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“…A 4-cm long midline incision was made on the abdominal wall. The serosal surface of the anterior wall of the cecum was abraded 15 mm in diameter with a sanding tip operated by a fl exible shaft rotary tool 10 (Fig. 1c,d).…”

Section: Surgical Techniquementioning

confidence: 99%

Development of a novel antiadhesive material, alginate flakes, ex vivo and in vivo

et al. 2011

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“…41 This one-component gel has proven utility in scar-free wound healing, post-surgical adhesion prevention, and stem cell expansion. Specifically, the one-component gels were used in vivo for vocal fold repair, 42 to reduce post-operative intra-abdominal and pericardial adhesions, 43,44 to eliminate postsurgical tendon adhesions, 45 to reduce airway stenosis, 46 to improve healing following endoscopic sinus surgery 47 and to promote healing following middle ear injury. 48 An example of the use of the PEGDAcrosslinked CMHA-S is illustrated in Figure 5.…”

Section: Deconstructing the Ecm: A Chemist's Solution To A Bioengineementioning

confidence: 99%

Engineering a clinically-useful matrix for cell therapy

Prestwich

2008

Organogenesis

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, biological, engineering, marketing, regulatory, and financial constraints. What is required is a biocompatible material for culture of cells in three-dimensions (3-D) that offers ease of use, experimental flexibility to alter composition and compliance, and a composition that would permit a seamless transition from in vitro to in vivo use. The challenge is to replicate the complexity of the native extracellular matrix (ECM) environment with the minimum number of components necessary to allow cells to rebuild a given tissue. Our approach is to deconstruct the ECM to a few modular components that can be reassembled into biomimetic materials that meet these criteria. These semi-synthetic ECMs (sECMs) employ thiol-modified derivatives of hyaluronic acid (HA) that can form covalently crosslinked, biodegradable hydrogels. These sECMs are "living" biopolymers, meaning that they can be crosslinked in the presence of cells or tissues to enable cell therapy and tissue engineering. Moreover, the sECMs allow inclusion of the appropriate biological cues needed to simulate the complexity of the ECM of a given tissue. Taken together, the sECM technology offers a manufacturable, highly reproducible, flexible, FDA-approvable, and affordable vehicle for cell expansion and differentiation in 3-D.

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Reduced postoperative intra-abdominal adhesions using Carbylan-SX, a semisynthetic glycosaminoglycan hydrogel

Cited by 67 publications

References 47 publications

Effects of extracellular matrix analogues on primary human fibroblast behavior

Effects of extracellular matrix analogues on primary human fibroblast behavior

Development of a novel antiadhesive material, alginate flakes, ex vivo and in vivo

Engineering a clinically-useful matrix for cell therapy

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