Reduced oxygen concentration during in vitro oocyte maturation alters global DNA methylation in the maternal pronucleus of subsequent zygotes in cattle

Bennemann, Johanna; Grothmann, Hanna; Wrenzycki, C.

doi:10.1002/mrd.23073

Cited by 12 publications

(11 citation statements)

References 71 publications

(81 reference statements)

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“…These results indicated that 5% CO 2 + 5% O 2 support the development of in vitro produced buffalo embryos to the blastocyst stage better than 20% O 2 conditions. These results are concomitant with those previously reported in cattle [9,23] and pigs [24]. Embryos cultured under OS showed higher ROS accumulation and higher abundance of transcripts related to OS response, which, in turn, decreases cell proliferation and number.…”

Section: Discussionsupporting

confidence: 90%

Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro

et al. 2021

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Aim: Oxidative stress (OS) is one of the major disruptors of oocyte developmental competence, which appears due to the imbalance between the production and neutralization of reactive oxygen species (ROS). Materials and Methods: In Experiment 1, buffalo oocytes were in vitro matured, fertilized, and cultured at 38.5°C under 5% CO2 + 20% O2 in standard CO2 incubator (OS) or under 5% O2 + 5% CO2 + 90% N2 (Multi-gas incubator, low O2). In Experiment 2, buffalo cumulus oocytes complexes (COCs) were matured in Basic maturation medium (BMM) composed of TCM199+ 10% FCS+ 10 μg/ml FSH+ 50 μg/ml gentamicin (control group) or in BMM supplemented with 50 μM ascorbic acid (ascorbic acid group) or 3.0 mM glutathione (glutathione group) or 10-5 M melatonin (melatonin group) and cultured at 38.5°C under 20% O2 for 24 h. Matured buffalo oocytes in control, ascorbic acid, or melatonin groups were fertilized and zygotes were cultured for 8 days under the same conditions. Results: In both experiments, maturation, cleavage, and blastocyst rates were recorded. Results showed that culture of buffalo oocytes under low O2 (5% O2) significantly increased maturation, cleavage, and blastocyst rates (p<0.05). Meanwhile, under 20% O2, addition of 10-5 M melatonin or 50 μM ascorbic acid to in vitro maturation (IVM) medium significantly improved cumulus cell expansion, nuclear maturation rates of buffalo oocytes (p<0.05), and increased cleavage and blastocyst rates (p<0.05). Conclusion: About 5% O2 is the optimum condition for in vitro production of buffalo embryos, and addition of 10-5 M melatonin to IVM medium for oocytes cultured under 20% O2 could alleviate the adverse effect of high oxygen tension and increased embryo yield.

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Section: Discussionsupporting

confidence: 90%

Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro

et al. 2021

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“…In addition, oxygen concentrations might have an effect on the epigenetic reprogramming of oocytes and preimplantation embryos. For example, a recent study reported a significant difference of 5-methylcytosine between maternal pronuclei of bovine oocytes matured at 5% and 20% oxygen (Bennemann et al, 2018). However, literature on the relationship between oxygen tension and epigenetic status are scarce and their long-term sequences still needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

“…However, literature on the relationship between oxygen tension and epigenetic status are scarce and their long-term sequences still needs to be further investigated. What is more, it must be mentioned that the actual dissolved oxygen concentration during IVM and IVC is generally lower than that in the incubator as the solubility of oxygen is poor in the culture medium (Bennemann et al, 2018;Talevi et al, 2018). The dissolved oxygen concentration is also affected by multiple factors such as the volume of the drop of culture medium, the amount of mineral oil applied, and the culture procedures (Sciorio and Smith, 2019).…”

Section: Discussionmentioning

confidence: 99%

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Luo

et al. 2020

Zygote

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Summary Oxygen concentration influences oocyte quality and subsequent embryo development, but it remains unclear whether oxygen concentrations affect the developmental competence and transcriptomic profile of yak oocytes. In this study, we investigated the effects of different oxygen concentrations (5% versus 20%) on the developmental competence, reactive oxygen species (ROS) levels, glutathione (GSH) content, and transcriptomic profile of yak oocytes. The results showed that a low oxygen concentration significantly increased the maturation rate of yak oocytes (81.2 ± 2.2% vs 75.9 ± 1.3%) and the blastocyst quality of yak in vitro fertilized embryos. Analysis of ROS and GSH showed that a low oxygen concentration reduced ROS levels and increased the content of GSH (75.05 ± 7.1 ng/oocyte vs 50.63 ± 5.6 ng/oocyte). Furthermore, transcriptomic analysis identified 120 differentially expressed genes (DEGs) between the two groups of oocytes. Gene enrichment analysis of the DEGs indicated multiple cellular processes, including oxidative phosphorylation, transcription regulation, mitochondrial regulation, oestrogen signalling pathway, HIF-1 signalling pathway, TNF signalling pathway, were involved in the response to oxygen concentration alterations. Taken together, these results indicated that a low oxygen concentration improved the developmental competence of yak oocytes.

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“…In vivo maturation. COCs were washed in 3 subsequent 100 μl drops of holding medium (0.453 g TCM199 supplemented with 1.5 mg gentamicin sulphate; 0.66 mg sodium pyruvate; 0.03 g NaHCO 3 ; 0,03 g BSA fatty acid free; dissolved in 30 ml sterile water) 75 . The transfer of COCs was conducted in a standardized volume using a Stripper micropipettor (The Stripper, MXL3-135, Origio a/s, Måløv, Denmark) adjusted to 2.5 μl.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro maturation. Collected immature COCs were washed individually in four consecutive 100 μl drops of maturation wash medium (0.453 g TCM199 supplemented with 1.5 mg gentamicin sulphate; 0.66 mg sodium pyruvate, 0.066 g NaHCO 3 ; 0.03 g BSA fatty acid free; dissolved in 30 ml sterile water) and transferred to 30 μl drops maturation medium (supplemented with 10 IU/ml equine chorionic gonadotrophin and 5 IU/ml human chorionic gonadotrophin) 75 . In vitro maturation was performed for 21 h in individual culture at 38.5 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte

Walter

Monthoux

Fortes

et al. 2020

Sci Rep

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In vitro maturation (IVM) of oocytes has still a negative impact on the developmental competence of oocytes. Therefore, this study analysed the cumulus proteome of individual cumulus-oocyte complexes (COCs) with and without maturational competence, matured under in vivo or in vitro conditions (n = 5 per group). A novel, ultrasensitive mass spectrometry (MS) based protein profiling approach, using label-free quantification, was applied. The detected cumulus proteome included 2226 quantifiable proteins and was highly influenced by the maturation condition (479 differentially expressed proteins) as well as maturational competence of the corresponding oocyte (424 differentially expressed proteins). Enrichment analysis showed an overrepresentation of the complement and coagulation cascades (CCC), ECM-receptor interaction and steroid biosynthesis in cumulus of COCs that matured successfully under in vivo conditions. Verification of the origin of CCC proteins was achieved through detection of C3 secretion into the maturation medium, with significantly increasing concentrations from 12 (48.4 ng/ ml) to 24 hours (68 ng/ml: p < 0.001). In relation, concentrations in follicular fluid, reflecting the in vivo situation, were >100x higher. In summary, this study identified important pathways that are impaired in IVM cumulus, as well as potential markers of the maturational competence of oocytes.

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Reduced oxygen concentration during in vitro oocyte maturation alters global DNA methylation in the maternal pronucleus of subsequent zygotes in cattle

Cited by 12 publications

References 71 publications

Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro

Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte

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