Reduced nucleic acid methylation impairs meiotic maturation and developmental potency of pig oocytes

Wang, Yankui; Yu, Xiaoxia; Liu, Yunhua; Li, Xuan; Liu, Xiaoman; Wang, Peichao; Liu, Shuai; Miao, Jia-Kun; Du, Zhiqiang; Yang, Caixia

doi:10.1016/j.theriogenology.2018.08.009

Cited by 32 publications

(21 citation statements)

References 39 publications

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“…In the present study, lower oxygen concentration increased the cell number per blastocyst and decreased blastocyst cell apoptosis, suggesting that low oxygen concentration was beneficial for yak embryo development. However, the blastocyst formation rate did not show statistical difference among the groups, which may be due to the complex nature of IVC, which is an orchestration of embryo developmental potential, in vitro culture medium, in vitro culture methods and embryo-embryo interactions (Sunde et al, 2016;Reader et al, 2017;Wang et al, 2018b;Lee et al, 2018).…”

Section: Discussionmentioning

confidence: 82%

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Luo

et al. 2020

Zygote

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Summary Oxygen concentration influences oocyte quality and subsequent embryo development, but it remains unclear whether oxygen concentrations affect the developmental competence and transcriptomic profile of yak oocytes. In this study, we investigated the effects of different oxygen concentrations (5% versus 20%) on the developmental competence, reactive oxygen species (ROS) levels, glutathione (GSH) content, and transcriptomic profile of yak oocytes. The results showed that a low oxygen concentration significantly increased the maturation rate of yak oocytes (81.2 ± 2.2% vs 75.9 ± 1.3%) and the blastocyst quality of yak in vitro fertilized embryos. Analysis of ROS and GSH showed that a low oxygen concentration reduced ROS levels and increased the content of GSH (75.05 ± 7.1 ng/oocyte vs 50.63 ± 5.6 ng/oocyte). Furthermore, transcriptomic analysis identified 120 differentially expressed genes (DEGs) between the two groups of oocytes. Gene enrichment analysis of the DEGs indicated multiple cellular processes, including oxidative phosphorylation, transcription regulation, mitochondrial regulation, oestrogen signalling pathway, HIF-1 signalling pathway, TNF signalling pathway, were involved in the response to oxygen concentration alterations. Taken together, these results indicated that a low oxygen concentration improved the developmental competence of yak oocytes.

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Section: Discussionmentioning

confidence: 82%

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Luo

et al. 2020

Zygote

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“…The differentially expressed m 6 A methylated genes were signi cantly enriched in several signaling pathways related with steroidogenesis, granulosa cell proliferation and follicular development [36]. Wang et al, reported that CL treatment impaired the maturation of porcine oocytes and reducing the RNA m 6 A level [37]. However, little is known about the effect of RNA m 6 A on the CCs expansion and ROS level of porcine oocytes.…”

Section: Discussionmentioning

confidence: 99%

m6a Methylation Inhibition by Cycloleucine Impaired Porcine Oocyte Meiosis and Early Embryonic Development via Remodeling Histone Modifications and Altering Metabolism Related Gene Expression in Blastocysts

Zhang¹,

Zhang²,

Zhai³

et al. 2020

Preprint

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BackgroundOocytes maturation and early embryo development were regulated precisely by a series of factors at transcriptional and posttranslational levels. N6-methyladenosine (m6A) is the most prevalent modification in mRNA as a crucial regulator in RNA metabolism and gene regulation. However, the role of m6A on porcine oocyte maturation and early embryogenesis is largely unknown. ResultsHere, we found that oocytes treated with cycloleucine (CL), an inhibitor of m6A, could impair cumulus expansion, elevate mitochondrial reactive oxygen species (ROS) concentration and decreased oocytes maturation which partially caused by disturbed spindle organization and chromosomes alignment. Moreover, our results indicated that the CL treated parthenogenetic embryos arrested at 4-cell stage and showed worse blastocyst quality. CL treatment not only decreased the methylation levels of nucleic acid, H3K4me3 and H3K9me3, while increased the acetylation level of H4K16 during parthenogenetic embryos development in pigs. Furthermore, single cell RNA-seq (scRNA-seq) analysis indicated that CL treatment dramatically elevated the expression of metabolism-related (SLC16A1 and MAIG3 etc.) and maternal related (BTG4, WEE2 and BMP15 etc.) genes at blastocyst stage. ConclusionsTaken together, we found that m6A methylation inhibition by CL impaired porcine oocyte meiosis and early embryonic development via remodeling histone modifications and altering metabolism related gene expression in blastocysts.

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“…M 6 A methyltransferases participate in germ cell maturation and preimplantation embryonic development in animals or plants and even in the reproduction of microorganisms [16]. Although mutations in METTL3 are lethal to embryos, limited research has been conducted on METTL3 in germ cells and more is needed [17,18], with scientists developing special methods to perform experiments.…”

Section: Growth and Developmentmentioning

confidence: 99%

Biological functions of m6A methyltransferases

Zhan

Zhuo

et al. 2021

Cell Biosci

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M6A methyltransferases, acting as a writer in N6-methyladenosine, have attracted wide attention due to their dynamic regulation of life processes. In this review, we first briefly introduce the individual components of m6A methyltransferases and explain their close connections to each other. Then, we concentrate on the extensive biological functions of m6A methyltransferases, which include cell growth, nerve development, osteogenic differentiation, metabolism, cardiovascular system homeostasis, infection and immunity, and tumour progression. We summarize the currently unresolved problems in this research field and propose expectations for m6A methyltransferases as novel targets for preventive and curative strategies for disease treatment in the future.

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Reduced nucleic acid methylation impairs meiotic maturation and developmental potency of pig oocytes

Cited by 32 publications

References 39 publications

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

m6a Methylation Inhibition by Cycloleucine Impaired Porcine Oocyte Meiosis and Early Embryonic Development via Remodeling Histone Modifications and Altering Metabolism Related Gene Expression in Blastocysts

Biological functions of m6A methyltransferases

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