Reduced muscle fiber force production and disrupted myofibril architecture in patients with chronic rotator cuff tears

Mendias, Christopher L.; Roche, Stuart M.; Harning, Julie A.; Davis, M. Edward; Lynch, Evan B.; Enselman, Elizabeth R. Sibilsky; Jacobson, Jon A.; Claflin, Dennis R.; Calve, Sarah; Bedi, Asheesh

doi:10.1016/j.jse.2014.06.037

Cited by 62 publications

(78 citation statements)

References 32 publications

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“…Steinbacher et al reported a similar increase in overall fat and perifascicular ECM, with increased lipid content and decreased myofibrillar organization in affected muscle fibers 17 . Mendias et al corroborated these findings, and further showed diminished force production in muscle fibers isolated from chronic, full-thickness tears 16 . Animal models of rotator cuff disease, particularly large animal models, are relatively effective in recapitulating this atrophic, fat-accumulating phenotype of early and midstage disease [29][30][31][32][33][34][35] .…”

mentioning

confidence: 80%

“…Regarding the terms fatty atrophy versus fatty degeneration, previous studies of the relationship between muscle loss and fat accumulation are limited and have focused primarily on early to mid-stage rotator cuff disease. Those studies demonstrated decreased muscle fiber diameter 15 and specific force production 16 along with disrupted subcellular architecture, including small (;1-mm) lipid droplet accumulation in individual muscle fibers following a tendon tear 16,17 . No degenerative features such as myophagocytosis, disrupted muscle fiber membranes, split fibers, centralized nuclei, or other classic features of muscle degeneration-regeneration 6 have been reported [15][16][17] .…”

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confidence: 99%

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Histological Evidence of Muscle Degeneration in Advanced Human Rotator Cuff Disease

Gibbons

Singh

Anakwenze

et al. 2017

The Journal of Bone and Joint Surgery

113

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Background: Cellular remodeling in rotator cuff muscles following a massive rotator cuff tear is poorly understood. The aim of the current study was to provide histological evidence to elucidate the mode of muscle loss in advanced human rotator cuff disease and to assess tissue-level changes in relation to findings on noninvasive imaging. Methods: Rotator cuff muscle biopsy samples were taken from the scapular fossae from 23 consecutive patients undergoing reverse total shoulder arthroplasty in order to evaluate muscle composition in severe rotator cuff disease. Markers of vascularity; inflammation; fat distribution; and muscle atrophy, degeneration, and regeneration were quantified. Results: The samples primarily consisted of dense, organized connective tissue (48.2% ± 19.1%) and disorganized, loose connective tissue (36.9% ± 15.9%), with substantially smaller fractions of muscle (10.4% ± 22.0%) and fat (6.5% ± 11.6%). Only 25.8% of the biopsy pool contained any muscle fibers at all. Increased inflammatory cell counts (111.3 ± 81.5 macrophages/mm2) and increased vascularization (66.6 ± 38.0 vessels/mm2) were observed across biopsies. Muscle fiber degeneration was observed in 90.0% ± 15.6% of observable muscle fascicles, and the percentage of centrally nucleated muscle fibers was pathologically elevated (11.3% ± 6.3%). Fat accumulation was noted in both perifascicular (60.7% ± 41.4%) and intrafascicular (42.2% ± 33.6%) spaces, with evidence that lipid may replace contractile elements without altering muscle organization. Conclusions: Dramatic degeneration and inflammation of the rotator cuff muscles are characteristics of the most chronic and severe rotator cuff disease states, suggesting that muscle loss is more complicated than, and distinct from, the simple atrophy found in less severe cases. Clinical Relevance: In order to address degenerative muscle loss, alternative therapeutic approaches directed at muscle regeneration must be considered if muscle function is to be restored in late-stage rotator cuff disease.

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confidence: 80%

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confidence: 99%

Histological Evidence of Muscle Degeneration in Advanced Human Rotator Cuff Disease

Gibbons

Singh

Anakwenze

et al. 2017

The Journal of Bone and Joint Surgery

113

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“…Examples include studies that have evaluated the effects of aging 12 , exercise 10,13,14 , spaceflight 15 , injury 2,3,16 , drug treatments 17,18 , disease 19 and genetic manipulation 20,21 on fiber structure and function. Due to the ability to directly assess the contractile performance of myofibrils in their native configuration, this technique provides an attractive platform from which to form an understanding of myofibrillar function absent of potentially confounding effects that are present when neuromuscular signal transmission and excitation-induced calcium release are included in the system under study.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, alternative means for exposing the fiber to the various experimental solutions could be employed, including devising a manually-operated system of chambers or a single chamber that allows for rapid filling and emptying of solutions. Finally, while sub-physiological experimental temperatures such as 15 °C are commonly used to improve the reproducibility of mechanical measurements 1,2,3,5,8,12,17,27 . Other approaches to solution composition have been employed with notable success by other research groups and typically make use of published binding constants and computational tools 27,30,31 .…”

Section: Discussionmentioning

confidence: 99%

“…Measurements of force using permeabilized skeletal muscle fibers are thus valuable when assessing alterations in muscle function observed in vivo. For example, we have used this technique to characterize the force generating capacity of fibers from myostatin deficient mice 1 and to assess the cause of persistent muscle weakness exhibited following chronic rotator cuff tears 2,3 .…”

Section: Introductionmentioning

confidence: 99%

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Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

Roche

Gumucio

Brooks

et al. 2015

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Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. Single muscle fiber studies are useful in both basic science and clinical studies. For basic studies, single muscle fiber contractility measurements allow investigation of fundamental mechanisms of force production, and analysis of muscle function in the context of genetic manipulations. Clinically, single muscle fiber studies provide useful insight into the impact of injury and disease on muscle function, and may be used to guide the understanding of muscular pathologies. In this video article we outline the steps required to prepare and isolate an individual skeletal muscle fiber segment, attach it to force-measuring apparatus, activate it to produce maximum isometric force, and estimate its cross-sectional area for the purpose of normalizing the force produced.

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Effects of shoulder abduction on the stiffness of supraspinatus muscle regions in rotator cuff tear

et al. 2021

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This study aimed to compare the effect of the load of the upper limb on the stiffness of supraspinatus muscle regions during isometric shoulder abduction in the scapular plane in healthy individuals and patients with a rotator cuff tear. Thirteen male patients were scheduled for arthroscopic rotator cuff repair, and 13 healthy male individuals were recruited. The movement task involved 30 isometric shoulder abduction in the scapular plane. The tasks included passive abduction, abduction with half-weight of the upper limb (1/2-weight), and full weight of the upper limb (fullweight). The stiffness of the supraspinatus muscle (anterior superficial, anterior deep, posterior superficial, and posterior deep regions) was recorded using ultrasound shear-wave elastography. The stiffness of the anterior superficial region on the affected side was significantly lower than that on the control side for the 1/2-weight and full-weight tasks. The stiffness of the anterior deep, posterior superficial, and

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Reduced muscle fiber force production and disrupted myofibril architecture in patients with chronic rotator cuff tears

Cited by 62 publications

References 32 publications

Histological Evidence of Muscle Degeneration in Advanced Human Rotator Cuff Disease

Histological Evidence of Muscle Degeneration in Advanced Human Rotator Cuff Disease

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

Effects of shoulder abduction on the stiffness of supraspinatus muscle regions in rotator cuff tear

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